The human breast cancer cell lines MDA-MB-231, BT-549, MDA-MB-157, HCC1937, MDA-MB-468 (American Type Culture Collection), SUM149, and SUM159 (Asterand Bioscience, Detroit, MI) were authenticated using a panel of microsatellite markers. Cell lines were maintained at 37°C in a humidified atmosphere of 5% CO2 in air as follows: MDA-MB-231 and BT-549 in RPMI 1640 (Sigma-Aldrich); MDA-MB-468 in Dulbecco's modified Eagle's medium (DMEM) (Lonza); MDA-MB-157 in Leibovitz (Lonza); SUM149 and SUM159 in DMEM F12 (Lonza) that was supplemented with insulin (5 μg/ml); and HCC1937 in RPMI 1640 medium that was supplemented with 1 mM sodium pyruvate, 1% (v/v) nonessential amino acids, and 10 mM Hepes. Each medium was also supplemented with 10% fetal bovine serum (FBS) and 2 mM glutamine (both from Sigma-Aldrich). For the stimulation with WHF, cells were starved in serum-free medium for 24 h and then treated for 48 h with a pool of 5 WHFs at a final concentration of 5% as described [24 (link)].
Sum149
Manufactured by Lonza
The SUM149 is a laboratory instrument designed for the analysis and processing of biological samples. It provides core functions for sample preparation and measurement, without interpretation or extrapolation of its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Dmem f12, by Lonza (2 mentions)
Mda mb 231, by Merck Group (1 mentions)
Mda mb 157, by American Type Culture Collection (1 mentions)
Hcc1937, by American Type Culture Collection (1 mentions)
Mda mb 468, by Lonza (1 mentions)
Bt549, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
Glutamine, by Merck Group (1 mentions)
Rpmi 1640, by Lonza (1 mentions)
Hcc1937, by Lonza (1 mentions)
Mcf 10a, by Lonza (1 mentions)
2 protocols using sum149
1
Culturing and Stimulating Breast Cancer Cell Lines
2
Culturing Breast Cancer Cell Lines
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The MCF10A human breast cell line was purchased from ATCC (Manassas, VA). SUM149 was purchased from Asterand (Detroit, MI). MCF10A was cultured in DMEM/F12 lonza (Walkersville, MD) supplemented with 5% horse serum, insulin (10 µg/mL), epidermal growth factor (20 ng/mL), cholera toxin (100 ng/ml), and gentamycin (50 ng/mL). SUM149 cells were cultured in DMEM/F12 lonza supplemented with 5% fetal bovine serum, insulin (5 µg/mL), and hydrocortisone (1mg/mL).
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