Infinite 200 pro nanoquant reader

Manufactured by Tecan

Sourced in Switzerland

The Infinite 200 PRO NanoQuant reader is a lab equipment product designed for the measurement of low-volume samples. It provides precise and accurate quantification of nucleic acids and proteins.

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Lab products found in correlation

Nanodrop one, by Thermo Fisher Scientific (2 mentions) 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions) 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Tecan (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Tecan (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions) Ncounter flex instrument, by NanoString (1 mentions) Qubit system, by Thermo Fisher Scientific (1 mentions) Nsolver 3, by NanoString (1 mentions) Tapestation 4200, by Agilent Technologies (1 mentions) Ncounter digital analyzer, by NanoString (1 mentions)

Spelling variants of this product

Infinite 200 PRO (2479 citations) Infinite 200 (774 citations) Infinite 200 PRO NanoQuant (157 citations) Infinite 200 PRO reader (89 citations) NanoQuant (32 citations) Infinite 200 reader (29 citations) Infinite 200 NanoQuant (22 citations) Infinite® 200 PRO NanoQuant plate reader (10 citations) Infinite PRO NanoQuant (6 citations) TECAN 200 Pro (2 citations)

4 protocols using infinite 200 pro nanoquant reader

ABTS Radical Scavenging Assay Protocol

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The measurement of 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) (Sigma-Aldrich, Germany) radical scavenging was carried out according to the protocol of Rufino et al. (2010)^{47 (link)} with some modifications. The ABTS radical solution was prepared by mixing 7.0 mmol/L ABTS and 2.5 mmol/L of potassium persulfate. Extracts of 10 μL were subsequently mixed with 290 μL of ABTS radical solution, and the absorbance of the resulting mixtures was measured after 30 min at 735 nm using Infinite 200 PRO NanoQuant reader (Tecan, Männedorf, Switzerland). The free radical-scavenging capacity was calculated by the following Equation (5):

Radical Scavenging [%] = (\frac{{Abs}_{B} - {Abs}_{S}}{{Abs}_{B}}) ⁎ 100 %

where, Abs_B is the absorbance of the ABTS mixed with deionized water; Abs_S is the absorbance of the ABTS mixed with extracts. All measurements were performed in triplicate and reported as the average value.

Junka A., Żywicka A., Chodaczek G., Dziadas M., Czajkowska J., Duda-Madej A., Bartoszewicz M., Mikołajewicz K., Krasowski G., Szymczyk P, & Fijałkowski K. (2019). Potential of Biocellulose Carrier Impregnated with Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite. Scientific Reports, 9, 1256.

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MTT Assay for Cell Viability

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Before each assay, fresh MTT solutions were prepared by dissolving 3 mg MTT (Sigma-Aldrich, Germany) in 10 mL pre-warmed (37 °C) PBS. One hundred μl PBS and 100 μL MTT solution were added to all wells. Plates were incubated in the dark for 1 h at 37 °C. In the next step, 100 µL of isopropanol was added to each well, and the plates vigorously shaken. The amount of MTT formazan formed during the incubation was measured with the Infinite 200 PRO NanoQuant reader (Tecan, Switzerland) at a wavelength of 570 nm and reference wavelength of 690 nm.

Junka A.F., Rakoczy R., Szymczyk P., Bartoszewicz M., Sedghizadeh P.P, & Fijałkowski K. (2018). Application of Rotating Magnetic Fields Increase the Activity of Antimicrobials Against Wound Biofilm Pathogens. Scientific Reports, 8, 167.

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DPPH Radical Scavenging Assay Protocol

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The measurement of 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Sigma-Aldrich, Germany) radical scavenging capacity was carried out according to Karamać et al.⁴⁸ 290 μl of 0.5 mmol/L DPPH in methanol (Meyer, México) was mixed with 10 μL of the extracts. The mixture was incubated for 30 min at room temperature followed by absorbance reading at 417 nm using Infinite 200 PRO NanoQuant reader (Tecan, Männedorf, Switzerland). The percentage of free radical-scavenging capacity was calculated by the following Equation (6):

Radical Scavenging Capacity [%] = (\frac{{Abs}_{B} - {Abs}_{S}}{{Abs}_{B}}) ⁎ 100 %

where, Abs_B is the absorbance of DPPH mixed with methanol instead of with the extracts; Abs_S is the absorbance of DPPH mixed with the extract. All measurements were performed in triplicate and reported as an average value.

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RNA Extraction and Expression Profiling

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Total RNA was extracted and purified using the High Pure formalin-fixed paraffin-embedded tissue RNA Isolation Kit (Roche, Indianapolis, IN) according to the manufacturer protocol. RNA was quantified and its quality was assessed using the Infinite 200 PRO NanoQuant reader (Tecan, Mannedorf, Switzerland) according to the manufacturer protocol.
Expression profiling of RNA samples (Table S2) was performed on the nCounter FLEX Instrument using the NanoPCIP panel of 770 genes (NanoString Technologies, Seattle, WA). Briefly, purified RNA was quantitated using the Qubit System (Life Technologies, Carlsbad, CA) and quality-checked using the NanoDrop One (Thermo Scientific, Waltham, MA) and TapeStation 4200 (Agilent, Santa Clara, CA). Fifty nanograms of RNA was hybridized to gene-specific, fluorescent-labeled probes that were then purified on the nCounter Prep Station. The fluorescent-labeled products were then scanned on the nCounter Digital Analyzer.
Gene expression data were analyzed by nSolver 3.0 software (NanoString Technologies), providing differential expression of all 770 genes in the NanoPCIP panel and immune cell profiling. Using expression of genes previously shown to be characteristic of various immune cell subsets, we estimated the abundance of 17 different immune cell subsets.

Hailemichael Y., Johnson D.H., Abdel-Wahab N., Foo W.C., Bentebibel S.E., Daher M., Haymaker C., Wani K., Saberian C., Ogata D., Kim S.T., Nurieva R., Lazar A.J., Abu-Sbeih H., Fa-ak F., Matthew A., Wang Y., Falohun A., Trinh V., Zobniw C., Spillson C., Burks J.K., Awiwi M., Elsayes K., Soto L.S., Melendez B.D., Davies M., Wargo J., Curry J., Yee C., Lizee G.A., Singh S., Sharma P., Allison J.P., Hwu P., Ekmekcioglu S, & Diab A. (2022). Interleukin-6 blockade abrogates immunotherapy toxicity and promotes tumor immunity. Cancer cell, 40(5), 509-523.e6.

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