Rats learnt to self-administer METH in standard sound-attenuated operant chambers (Med Associates, VT, USA). Each chamber had two retractable levers, a cue light above each lever, a house light and a tone generator. Each lever was programmed as either active or inactive and their position was counterbalanced across chambers. Four infrared photobeam detectors were also positioned on the sidewall of each operant chamber to measure locomotor activity. Active and inactive lever presses, number of infusions and locomotor activity were recorded using MED-PC-V software (Med Associates). Locomotor activity was recorded during all addiction-relevant stages, except for persistence in drug-seeking when the drug was signalled not available.
Sound attenuated operant chambers
Manufactured by Med Associates
Sourced in United States
Sound-attenuated operant chambers are enclosures designed to provide a controlled environment for behavioral research experiments. These chambers are engineered to minimize external noise and disturbances, creating a isolated setting for precise observation and data collection.
Automatically generated - may contain errors
5 protocols using sound attenuated operant chambers
1
Methamphetamine Self-Administration in Rats
2
Operant Conditioning in Sound-Attenuated Chambers
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All behavioral procedures were carried out in sound-attenuated operant chambers (29.53 × 23.5 × 27.31 cm) equipped with a chamber light and speakers for tone and white noise (Med Associates Inc., Fairfax, VT). One wall of the chamber had a port in the center which consisted of a receptacle with a head entry detector. A syringe pump connected with plastic tubing to the receptacle was used to deliver solutions to the receptacle. All behavioral events (lights, cue presentations, and syringe pump) were controlled by the MED-PC software (Med Associates).
3
Operant Conditioning Behavioral Assay
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All behavioral procedures were carried out in sound-attenuated operant chambers (29.53 × 23.5 × 27.31 cm) equipped with a chamber light and speakers for tone and white noise (Med Associates Inc., Fairfax, VT). One wall of the chamber had a port in the center which consisted of a receptacle with a head entry detector. A syringe pump connected with plastic tubing to the receptacle was used to deliver solutions to the receptacle. All behavioral events (lights, cue presentations, and syringe pump) were controlled by the MED-PC software (Med Associates).
4
Operant Self-Administration Behavioral Procedure
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Self-administration training and testing was conducted in sound-attenuated operant chambers (MED Associates Inc., Vermont, USA) equipped with two stimulus lights above two retractable stainless steel levers. A white house light placed on the wall opposite the levers was on during the entire session. A ventilating fan operated throughout the sessions and served as a masking noise. Intravenous solutions were delivered using an infusion pump (PHM-100, 3.33 rpm; Med Associates Inc.), and a 10-mL plastic syringe placed in the pump was connected to the implanted catheter through CoEx tubing (Harvard Apparatus, Kent, UK) and protected by a flexible metal leash (CamCaths, Ely, UK). Experiments were run and data collected by a PC with the MED-PC software (MedPC IV, MED Associates Inc., Vermont USA). The experiments were conducted in the same boxes for both training and test sessions, and ethanol- and water-treated animals were processed simultaneously throughout all phases of the self-administration procedure.
5
Fentanyl Self-Administration in Rats
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Animals were trained to self-administer fentanyl on a 2h fixed ratio 1 (FR1) schedule in soundattenuated operant chambers (Med Associates, St. Albans, VT, USA), as described previously . Drug availability was signaled by illumination of the house light, and an infusion of fentanyl (3.6 s infusion of 0.5 μg of fentanyl, iv) was elicited when the active lever was pressed. Drug infusions were paired with illumination of a white stimulus light above the active lever, and tone (78 dB, 2900 Hz), both of which were presented for the duration of the infusion. Inactive lever responses were recorded but did not result in infusions or cues. Each infusion was followed by a 20 s time out, which was signaled by the house light offset. The operant chambers were controlled by Med-PC IV software. Animals were trained for a minimum of 10 sessions (1 session per day) and to a criterion of >25 infusion for 3 consecutive days.
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