The following chemicals, unless specifically indicated, were from Sigma-Aldrich (St. Louis, MO, United States): forskolin (F6886), epinephrine (E4642), heparin (H3149), 4', 6-diamidino-2 phenylindole dihydrochloride (DAPI, D8417), mitomycin C (M5353), and dimethylsulfoxide (D2650). Anti ERK2 (rabbit, 1:1000, sc-292838, RRID: AB_2650548; Santa Cruz Biotechnology, Dallas, TX, United States) and anti-zyxin (mouse, 1:500, H00007791-M01, RRID: AB_2221180; Abnova, Taipei, Taiwan) were used for western blot. Anti Pecam1 (rat, 1:500, 553370, RRID: AB_394816; BD Bioscience, San Jose, CA, United States) and anti Ki67 (rabbit, 1:500, ab15580, RRID: AB_443209; Abcam, Cambridge, MA, United States) were used for whole-mount staining. anti-zyxin (mouse, 1:500, H00007791-M01, RRID: AB_2221180; Abnova, Taipei, Taiwan) and anti Ki67 (rabbit, 1:500, ab15580, RRID: AB_443209; Abcam, Cambridge, United Kingdom) were used for immunohistochemistry and immunofluorescence staining.
Kang X., Deng Y., Cao Y., Huo Y, & Luo J. (2021). Zyxin Mediates Vascular Repair via Endothelial Migration Promoted by Forskolin in Mice. Frontiers in Physiology, 12, 741699.
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