CD80loMHCIIlo immature mTECs were sorted on a FACSAria Fusion (BD Biosciences) into complete RPMI, followed by stimulation with 500 ng/ml recombinant mouse RANKL (BioLegend) for 6 h at 37°C.
Recombinant mouse rankl
Manufactured by BioLegend
Recombinant mouse RANKL is a protein produced in a laboratory. It is a member of the tumor necrosis factor (TNF) ligand family and plays a key role in the regulation of bone metabolism.
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Lab products found in correlation
2 protocols using recombinant mouse rankl
1
RANKL Stimulation of Immature mTECs
2
Osteoclastogenesis Induction Protocol
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To induce osteoclastogenesis, both RAW 264.7 cells and BMDMs were stimulated with 3 and 10 ng/ml, respectively, of recombinant mouse RANKL (Biolegend, Cat# 769406), in the presence or absence of 1 μg/ml and 10 ng/ml, respectively, of PGDHC isolated from Porphyromonas gingivalis (ATTC, strain #33277), which was provided by Dr. Frank Nichols (University of Connecticut, Mansfield, CT). For biological experiments, PGDHC was sonicated (2 s, 3 W) in phosphate‐buffered saline (PBS, Gibco, Cat# 10010049) to achieve a concentration of 1 μg/ml. In addition, specific experimental groups were treated with 1 μM of intracellular CatB inhibitor, CA‐074‐ME (Calbiochem, Cat# 205531).
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