Donkey anti goat igg h l alexa fluor 488 conjugate

Manufactured by Thermo Fisher Scientific

Donkey anti-goat IgG (H+L) Alexa Fluor 488 conjugate is a secondary antibody that binds to goat immunoglobulin G (IgG) and is conjugated with Alexa Fluor 488 fluorescent dye. It can be used for detection and visualization of goat primary antibodies in various immunoassays and microscopy applications.

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Lab products found in correlation

P75ntr, by Merck Group (2 mentions) Cy3 donkey anti rabbit igg h l, by Jackson ImmunoResearch (2 mentions) Gapdh, by Merck Group (2 mentions) Serine 63 phosphorylated c jun, by Cell Signaling Technology (2 mentions) Calnexin, by Enzo Life Sciences (2 mentions) Sox10, by R&D Systems (2 mentions) C jun, by Cell Signaling Technology (2 mentions) Deae dextran hydrochloride, by Merck Group (1 mentions) Imaging chamber, by Ibidi (1 mentions) Anti gfp, by Abcam (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions) Anti gamma h2a x phospho s139, by Abcam (1 mentions)

Close spelling variants of this product

Alexa Fluor 488 (6806 citations) Alexa 488 (1863 citations) Alexa Fluor 488 conjugate (271 citations) Alexa Fluor 488 donkey anti-goat IgG (117 citations) Alexa Fluor 488 donkey anti-goat (98 citations) Alexa Fluor conjugates (56 citations) Donkey anti-goat IgG (46 citations) Alexa 488 donkey-anti-goat (34 citations) Alexa Fluor 488 anti-goat IgG (28 citations) Donkey anti-goat 488 (23 citations)

4 protocols using donkey anti goat igg h l alexa fluor 488 conjugate

Immunofluorescence and Western Blotting Protocols

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Immunofluorescence antibodies: c-Jun (Cell Signaling Technology, rabbit 1:800), Sox10 (R and D Systems, goat 1:100), CGRP (Peninsula, rabbit 1:1000), neurofilament (Abcam, rabbit 1:1000), donkey anti-goat IgG (H+L) Alexa Fluor 488 conjugate (Invitrogen, 1:1000), and Cy3 donkey anti-rabbit IgG (H+L) (Jackson Immunoresearch, 1:500).
Antibodies used for western blotting: c-Jun (Cell Signaling Technology, rabbit 1:1000), p75 NTR (Millipore, rabbit 1:1000), serine 63 phosphorylated c-Jun (Cell Signaling Technology, rabbit 1:1000), GAPDH (Sigma-Aldrich, rabbit 1:5000), calnexin (Enzo Life Sciences, rabbit 1:1000), and anti-rabbit IgG, HRP-linked (Cell Signaling Technology, 1:2000).

Wagstaff L.J., Gomez-Sanchez J.A., Fazal S.V., Otto G.W., Kilpatrick A.M., Michael K., Wong L.Y., Ma K.H., Turmaine M., Svaren J., Gordon T., Arthur-Farraj P., Velasco-Aviles S., Cabedo H., Benito C., Mirsky R, & Jessen K.R. (2021). Failures of nerve regeneration caused by aging or chronic denervation are rescued by restoring Schwann cell c-Jun. eLife, 10, e62232.

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Immunolabeling and Western Blotting Protocols

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Immunofluorescence antibodies: c-Jun (Cell Signaling Technology, rabbit 1:800), Sox10 (R&D Systems, goat 1:100), CGRP (Peninsula, rabbit 1:1000), Neurofilament (Abcam, rabbit 1:1000) donkey anti-goat IgG (H+L) Alexa Fluor 488 conjugate (Invitrogen, 1:1000), Cy3 donkey anti-rabbit IgG (H+L) (Jackson Immunoresearch, 1:500).
Antibodies used for Western blotting: c-Jun (Cell Signaling Technology, rabbit 1:1000), p75 NTR (Millipore, rabbit 1:1000), serine 63 phosphorylated c-Jun (Cell Signaling Technology, rabbit 1:1000), GAPDH (Sigma-Aldrich, rabbit 1:5000), calnexin (Enzo Life Sciences, rabbit 1:1000), anti-rabbit IgG, HRP-linked (Cell Signaling Technology, 1:2000) .

Wagstaff L., Gomez‐Sanchez J.A., Fazal S.V., Otto G., Kilpatrick A.M., Michael K., Wong L.Y., H. K., Turmaine M., Svaren J., Gordon T., Arthur‐Farraj P., Velasco-Avilés S., Cabedo H., Benito C., Mirsky R., & Jessen K.R. (2020). Failures of nerve regeneration caused by aging or chronic denervation are rescued by restoring Schwann cell c-Jun.

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Cornea Infection via HSV-1 Virus

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Corneas cultured for 2 weeks were transferred in culture medium containing SC16 or rHSV-1 (five corneas for each condition). Excess virus particles were discarded 3 h later and corneas were either incubated in DMEM supplemented with FCS and DEAE-dextran hydrochloride (5 μg/mL, Sigma #D9885) for infection in liquid medium, or cultured in a 3:1 mix of DMEM DEAE-dextran (5 μg/mL), supplemented with an aqueous solution of Lonza SeaKem ME agar (final concentration: 0.6%) for infection under agar. Corneas were fixed in 0.5% PFA and processed 40 h later for CMRA fluorescence, X-Gal-chromophore release or immunolabeled with antibodies targeting the HSV-1 envelope glycoprotein D (gD) and counterstained with DAPI. The anti-HSV-1 labeling included permeabilization with 0.05% Triton X-100, incubation with the primary antibody (goat antibody HSV-1 gD [vN-20], Santa Cruz Biotechnology #SC-17540; diluted 1:100 in D-PBS containing 0.01% Triton X-100 and 1% BSA) and then with the secondary antibody (donkey anti-goat IgG [H + L] Alexa Fluor 488 conjugate, ThermoFisher Scientific #A-11055; diluted 1:1000). Endothelial surfaces were examined with a macroscope.

Chapellier B., Guindolet D., Pereira D., Galetto R., Sahel J.A., Labetoulle M, & Gabison E.E. (2022). Meganuclease targeting HSV-1 protects against herpetic keratitis: Application to corneal transplants. Molecular Therapy. Nucleic Acids, 30, 511-521.

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DNA Damage Quantification in Transfected Cells

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293T cells were transfected with pLVX-overexpression or vector control and seeded onto imaging chambers (Ibidi Cat #: 81156) pre-coated with poly-D-lysine. At about 70 h post-transfection, cells were fixed with 3.7% formaldehyde in DPBS for 15 min at room temperature, washed twice with DPBS, permeabilized and blocked in DPBS supplemented with 0.5% Triton X and 1% BSA for 15 min at room temperature, and incubated overnight at 4 degC with primary antibodies anti-gamma H2A.X (phospho S139) (Abcam, Cat #: ab2893, 1:1000), and anti-GFP (Abcam, Cat #: 6673, 1:500). On the following day, the imaging chambers are washed thrice with DPBS, and incubated with secondary antibodies donkey anti-Goat IgG (H + L) Alexa Fluor® 488 conjugate (Thermofisher, Cat #: A11055, 1:500) and donkey anti-Rabbit IgG (H + L) Alexa Fluor® 594 conjugate (Thermofisher, Cat #: A21207, 1:250), and DAPI (1 μg/mL) for 1.5 h at room temperature. The chambers were washed thrice with DPBS before imaging using Zeiss LSM700 confocal microscope.

Lau M.S., Hu Z., Zhao X., Tan Y.S., Liu J., Huang H., Yeo C.J., Leong H.F., Grinchuk O.V., Chan J.K., Yan J, & Tee W.W. (2023). Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription. Nature Communications, 14, 6464.

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