The primary antibodies used in this study were polyclonal antibodies against human β‐ACTIN, RASSF1 (Santa Cruz, CA, USA), ERK, phospho‐ERK (Thr202/Tyr204), caspase‐3 and PARP (Cell Signaling Technology, Beverly, MA, USA). HRP‐labeled secondary antibodies were used for the detection of primary antibodies and the bands were detected by ImageQuant LAS4000 (GE Healthcare UK, Little Chalfont, UK).
Rassf1
Manufactured by Santa Cruz Biotechnology
Sourced in United States, United Kingdom
RASSF1 is a protein that plays a role in cell signaling pathways. It is involved in the regulation of cellular processes such as cell cycle control, apoptosis, and cellular responses to DNA damage. RASSF1 is considered a tumor suppressor gene and has been studied in the context of cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Cyclin d1, by Cell Signaling Technology (2 mentions)
P mob1, by Cell Signaling Technology (2 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Imagequant las 4000, by GE Healthcare (1 mentions)
P gsk3β, by Cell Signaling Technology (1 mentions)
P pten, by Cell Signaling Technology (1 mentions)
3 methyladenine 3 ma, by Merck Group (1 mentions)
Mmp 9, by Cell Signaling Technology (1 mentions)
Dmem f12 media, by Thermo Fisher Scientific (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Ly294002, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
P smad, by Cell Signaling Technology (1 mentions)
P lats1, by Cell Signaling Technology (1 mentions)
Lats1, by Cell Signaling Technology (1 mentions)
3 protocols using rassf1
1
Western Blot Antibody Staining
2
Esophageal Cancer Cell Lines Analyses
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The esophageal cancer cell lines TE-8 and TE-12 were obtained from Dr. Izzo (University of Texas MD Anderson Cancer Center) and were grown in DMEM-F12 media (Gibco) with FBS (Welgene) and penicillin-streptomycin (Sigma). Primary antibodies against YAP, Mst1, Mst2, caspase-9, c-caspase-9, p-YAP, PARP, c-PARP, Akt, p-Akt, Mob-1, p-Mob1, p-GSK-3β, E-cadherin, MMP-9, CDK4, CDK6, cyclin D1, Sav1, p-PTEN, PTEN, and GAPDH were obtained from Cell Signaling Technology, and primary antibodies against MMP-13, Rassf1, and CTGF were purchased from Santa Cruz Biotechnology Inc. UA was obtained from Cayman Chemical Company, and DIM was purchased from LKT Laboratories. 3-Methyladenine (3-MA) and LY294002 were obtained from Sigma Chemicals and Cell Signaling Technology, respectively. Both were dissolved in dimethyl sulfoxide (DMSO) (Sigma Chemical Co.).
3
Esophageal Cancer Cell Signaling Pathway
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Recombinant human BMP-2 was purchased from DaeWoong Pharmaceutical (Seoul, South Korea). Cell cycle related protein antibodies (cyclin D1, CDK4, p21, p53, p-Smad, and CDK6) and Hippo signaling pathway related protein antibodies (Mst1, Mst2, Sav1, LATS1, p-LATS1, Mob1, p-Mob1, YAP, and p-YAP) were obtained from Cell Signaling Technology (Danvers, MA, USA). The BMP-2 antibody was obtained from Abcam (Cambridge, UK) and BMPR II, RASSF1, Akt, p-Akt, TP63, and β-actin antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). RASSF1 small interfering RNA (siRNA), YAP siRNA, or control siRNA were purchased from Santa Cruz biotechnology (Dallas, TX, USA). The human TE-8 and TE-12 esophageal cancer cell lines were obtained from Dr. Izzo (Unversity of Texas M.D. Anderson Cancer Center, Houston, TX, USA). DMEM-F12 medium (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco), 100 mg/ml streptomycin, and 100 IU/ml penicillin (Gibco) was used for cell medium. Cells were maintained under standard conditions at 37 °C in a 5% CO2 humidified atmosphere.
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