We isolated peripheral blood mononuclear cells (PBMCs) from the blood samples using density-gradient centrifugation on Ficoll-Paque. Single-cell suspensions were treated with fixable viability stain 510 (BD Bioscience, 564406) for half an hour, followed by staining with the following antibodies to distinguish the Tfh subsets: FITC- conjugated anti-CD45RA (BioLegend, N418), PE-conjugated anti-CCR6 (BioLegend, G034E3), PerCP/Cy5.5-conjugated anti-CXCR5 (BioLegend, J252D4), PE/Cy7-conjugated anti-CXCR3 (BioLegend, G025H7), APC-conjugated anti-PD-1 (BioLegend, EH12.2H7), and APC/Cy7- conjugated anti-CD4 (BioLegend, RPA-T4). For the detection of circulating plasma cells, PBMCs were stained with FITC-conjugated CD27 (BioLegend, M-T271), PE-conjugated IgD (BioLegend, W18340F), PerCP/Cy5.5-conjugated anti-CD19 (BioLegend, HIB19), and APC-conjugated CD38 (BioLegend, HB-7), followed by the treatment with fixable viability stain 510. Cell acquisition was performed using a CantoII cytometer (BD Bioscience). Data were analyzed with Diva (BD) software.
Fitc conjugated anti cd45ra
Manufactured by BioLegend
FITC-conjugated anti-CD45RA is a monoclonal antibody that binds to the CD45RA antigen on the surface of T cells. CD45RA is a marker expressed on naive T cells, and this antibody can be used to identify and characterize this cell population.
Automatically generated - may contain errors
Lab products found in correlation
Canto 2 cytometer, by BD (1 mentions)
Fixable viability stain 510, by BD (1 mentions)
Apc cy7 conjugated anti cd4, by BioLegend (1 mentions)
Percp cy5.5 conjugated anti cd19, by BioLegend (1 mentions)
Apc conjugated anti pd 1, by BioLegend (1 mentions)
Fitc conjugated cd27, by BioLegend (1 mentions)
Apc conjugated anti cd38, by Thermo Fisher Scientific (1 mentions)
7 aminoactinomycin d, by BioLegend (1 mentions)
2 protocols using fitc conjugated anti cd45ra
1
Flow Cytometric Analysis of Tfh Subsets and Plasma Cells
2
Viral Specificity Characterization via Tetramer Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To further characterize virus specificity, we performed EBV and CMV tetramer staining in patients for whom HLA typing was amenable to available viral-specific tetramer staining. We had samples available for 16 patients in the acute GVHD cohort and 8 patients in the no acute GVHD cohort (Supplementary Table 1). In brief, 2 £ 1 £ 10 6 thawed PBMCs were stained with appropriate PE-conjugated tetramers with peptide sequences and specificities shown in Supplementary Table Biosciences), APC-conjugated anti-CD38 (eBioscience), FITC-conjugated anti-CD45RA, and 7-amino-actinomycin D (BioLegend) to assess for viability.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!