The entire middle part of the penis was fixed by immersion in 4% paraformaldehyde and dehydrated overnight in PBS containing 30% sucrose. The tissue was evenly sliced into 5-µm sections and placed on slides. The sections were processed with primary antibodies against Gal-3 (1:500; Abcam), TLR4 (1:100; Abcam), MyD88 (1:100; Proteintech), and NF-κB-65 (1:500; Cell Signaling Technology) for 1 h. The sections were then washed and treated with Alexa Fluor 594-labeled secondary antibodies (Invitrogen, Carlsbad, CA, USA). To stain the nuclei, the sections were incubated with DAPI (Thermo Fisher, MA, USA) for 5 min. A microscope was then used to analyze the fluorescence.
Nfκb 65
Manufactured by Cell Signaling Technology
Sourced in Japan
NFκB-65 is a lab equipment product offered by Cell Signaling Technology. It is used to detect and quantify the levels of the p65 subunit of the NF-κB transcription factor. NF-κB plays a crucial role in regulating the immune response, inflammation, and cell survival.
Automatically generated - may contain errors
Lab products found in correlation
Hcc1937, by National Collection of Authenticated Cell Cultures (2 mentions)
Erk1 2, by Cell Signaling Technology (2 mentions)
P erk1 2, by Cell Signaling Technology (2 mentions)
Gapdh antibody, by Santa Cruz Biotechnology (2 mentions)
Mda mb 231, by National Collection of Authenticated Cell Cultures (2 mentions)
β catenin, by Cell Signaling Technology (2 mentions)
P nfκb 65, by Cell Signaling Technology (2 mentions)
P akt1, by Cell Signaling Technology (2 mentions)
P β catenin, by Cell Signaling Technology (2 mentions)
Alexa fluor 594 labeled secondary antibody, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Myd88, by Proteintech (1 mentions)
Gal 3, by Abcam (1 mentions)
Sybr master mixture, by Takara Bio (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hcc1937, by Thermo Fisher Scientific (1 mentions)
3 protocols using nfκb 65
1
Immunohistochemical Analysis of Penile Tissue
2
Epithelial-Mesenchymal Transition Mechanism
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HCC1937 and MDA-MB-231 cell lines were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Genview (Campbellfield, VIC, Australia); SYBR Master Mixture was purchased from Takara (Shimogyo-ku, Kyoto, Japan); antibodies against CCT3, CDH1, Slug, Snail, VIM, mTOR, ERK1/2, p-ERK1/2, p-AKT1, P38, p-P38, NFκB-65, p-NFκB-65, β-catenin and p-β-catenin were purchased from Cell Signaling Technology (Danvers, MA, USA); and antibodies against CDH2, MMP2, FN1, MYC, p-mTOR and AKT1 were purchased from Abcam (Cambridge, MA, USA). GAPDH antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).
3
Breast Cancer Cell Line Characterization
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Cells and materials HCC1937 and MDA-MB-231 cell lines were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Genview (Campbell eld, VIC, Australia); SYBR Master Mixture was purchased from Takara (Shimogyo-ku, Kyoto, Japan); antibodies against CCT3, CDH1, Slug, Snail, VIM, mTOR, ERK1/2, p-ERK1/2, p-AKT1, P38, p-P38, NFκB-65, p-NFκB-65, β-catenin and p-β-catenin were purchased from Cell Signaling Technology (Danvers, MA, USA); and antibodies against CDH2, MMP2, FN1, MYC, p-mTOR and AKT1 were purchased from Abcam (Cambridge, MA, USA). GAPDH antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).
Cell culture HCC1937 and MDA-MB-231 cell lines were cultured in RPMI 1640 medium(Gibco; Gaithersburg, MD, United States) supplemented with 10% foetal bovine serum. The cells were maintained at 37°C in a 5% CO 2 humidi ed incubator.
Cell culture HCC1937 and MDA-MB-231 cell lines were cultured in RPMI 1640 medium(Gibco; Gaithersburg, MD, United States) supplemented with 10% foetal bovine serum. The cells were maintained at 37°C in a 5% CO 2 humidi ed incubator.
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