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Clone ep459y

Manufactured by Thermo Fisher Scientific
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The Clone EP459Y is an antibody produced for research use. It is developed by Thermo Fisher Scientific and can be used for the identification of the target protein in various applications.

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Lab products found in correlation

Clone 4b11, by Leica (2 mentions) Dmi8 inverted microscope, by Leica (2 mentions) Anti rabbit and anti mouse hrp secondary antibodies, by Abcam (2 mentions)

2 protocols using clone ep459y

1

Quantifying GBM B Cell Infiltration

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A total of 60 GBM patients were analyzed for the presence of CD20+ B cells by IHC. Formalin-fixed paraffin-embedded tumor blocks of GBM patients (see Table 1 for patients’ characteristics) were double-labeled with rabbit anti-human CD20 (1:2 dilution, clone EP459Y, Thermo Fischer) and mouse anti-human CD8 (1:100 dilution, clone 4B11, Leica). Anti-rabbit and anti-mouse HRP secondary antibodies (both from Abcam) were used at 1:500 dilution. Twenty consecutive high-power fields were evaluated on each tumor by a board-certified neuropathologist from the Neurosurgical Department at Northwestern University. Images were captured on a Leica DMi8 inverted microscope.
Lee-Chang C., Rashidi A., Miska J., Zhang P., Pituch K.C., Hou D., Xiao T., Fischietti M., Kang S.J., Appin C.L., Horbinski C., Platanias L.C., Lopez-Rosas A., Han Y., Balyasnikova I.V, & Lesniak M.S. (2019). Myeloid-derived suppressive cells promote B cell–mediated immunosuppression via transfer of PD-L1 in glioblastoma. Cancer immunology research, 7(12), 1928-1943.
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2

Quantifying Tumor-Infiltrating Lymphocytes in GBM

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Formalin-fixed paraffin-embedded tumor blocks of 60 GBM patients were double-labeled with rabbit anti-human CD20 (1:2 dilution, clone EP459Y, Thermo Fischer) and mouse anti-human CD8 (1:100 dilution, clone 4B11, Leica). Anti-rabbit and anti-mouse HRP secondary antibodies (both from Abcam) were used at 1:500 dilution. Twenty consecutive high-power fields were evaluated on each tumor by a board-certified neuropathologist from the Neurosurgical Department at Northwestern University. Images were captured on a Leica DMi8 inverted microscope.
Hou D., Wan H., Katz J.L., Wang S., Castro B.A., Vazquez-Cervantes G.I., Arrieta V.A., Dhiantravan S., Najem H., Rashidi A., Chia T.Y., Arjmandi T., Collado J., Billingham L., Lopez-Rosas A., Han Y., Sonabend A.M., Heimberger A.B., Zhang P., Miska J, & Lee-Chang C. (2024). Antigen-presenting B cells promote TCF-1+ PD1- stem-like CD8+ T-cell proliferation in glioblastoma. Frontiers in Immunology, 14, 1295218.
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