Anti snai1

Manufactured by Abcam

Sourced in United States

Anti-SNAI1 is a primary antibody that specifically recognizes the SNAI1 protein. SNAI1 is a transcription factor involved in the regulation of epithelial-mesenchymal transition (EMT) during embryonic development and cancer metastasis.

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3 protocols using anti snai1

Epithelial-Mesenchymal Transition Markers

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Two capsular bags were homogenized in each group, and then the homogenates were assayed with SDS-polyacrylamide gels and transferred to polyvinyl difluoride membranes (Thermo Fisher Scientific). The membranes were stripped and probed with the following primary antibodies: anti-GAPDH (Kangchen), anti-SNAI1 (Abcam), anti-E-Cadherin (BD Transduction Laboratories), anti-Vimentin (Abcam), and anti-alpha-SMA (Abcam). Quantification of Western blots was processed with ImageJ.

Zhang L., Wang Y., Li W., Tsonis P.A., Li Z., Xie L, & Huang Y. (2017). MicroRNA-30a Regulation of Epithelial-Mesenchymal Transition in Diabetic Cataracts Through Targeting SNAI1. Scientific Reports, 7, 1117.

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Evaluating Epithelial-Mesenchymal Transition

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Where indicated, the following drugs were used: MG132 (10 μM, Sigma, Saint Louis, MO, USA), cycloheximide (20 nM, Sigma, Saint Louis), CsA (2 μg/ml or 20 μg/ml, Sangon, Shanghai, China), and Leptomycin B (5 ng/ml, Beyotime, Shanghai, China). Rabbit anti-Flag, anti-GFP, anti-HA, and mouse anti-Flag antibodies were purchased from Sigma (Sigma, Saint Louis, MO, USA). Mouse anti-E-cadherin, anti-Cytokeratin 18, and rabbit anti-F-actin and anti-Vimentin antibody, rabbit anti-N-cadherin, and anti-SNAI1 antibodies were obtained from Abcam (Abcam, Cambridge, MA, USA). Rabbit anti-PPIL2 antibodies were purchased from Thermo Fisher Scientific (Thermo Fisher, Massachusetts, USA). Anti-fibronectin antibody was purchased from Wanleibio (Wanleibio, Shenyang, China).

Jia Z., Wang M., Li S., Li X., Bai X.Y., Xu Z., Yang Y., Li B., Li Y, & Wu H. (2018). U-box ubiquitin ligase PPIL2 suppresses breast cancer invasion and metastasis by altering cell morphology and promoting SNAI1 ubiquitination and degradation. Cell Death & Disease, 9(2), 63.

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Western Blotting and Cell Migration Assays

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Western blot analysis. Western blot analysis was performed as described before (27) . Briefly, after incubation with primary antibody anti-SMURF2 (1:250; Abcam, Cambridge, MA, USA), anti-E-cadherin (1:500; Abcam), anti-N-cadherin (1:200; Abcam), anti-vimentin (1:500; Abcam), anti-SNAI1 (1:500; Abcam), anti-TGFB1 (1:800; Abcam), anti-TWIST (1:500; Abcam), anti-ZEB1 (1:500; Abcam), anti-TGFB2 (1:500; Abcam), anti-α-SMA (1:500; Abcam) and anti-β-actin (1:500; Abcam) overnight at 4˚C, IRDye™-800 conjugated anti-rabbit secondary antibodies (Li-COR, Biosciences, Lincoln, NE, USA) were used for 30 min at room temperature. The specific proteins were visualized by Odyssey™ Infrared Imaging System (Gene Co., Lincoln, NE, USA).
Migration and invasion assay. For transwell migration assays, 5x10 4 cells were plated in the top chamber with the non-coated membrane (24-well insert; pore size, 8 mm; BD Biosciences, San Jose, CA, USA). For invasion assays, 1.25x10

Zhang W.L., Zhang J.H., Wu X.Z., Yan T, & Lv W. (2015). miR-15b promotes epithelial-mesenchymal transition by inhibiting SMURF2 in pancreatic cancer. International journal of oncology, 47(3).

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