Uv 5300

Ultraviolet (UV) spectrum: BAI, DG M, and BAI-DG M solutions were placed into a quartz cuvette for a UV–Vis spectrophotometer (UV-5300, Hitachi, Tokyo, Japan) to record the spectra between 200 and 500 nm.
Fourier transform infrared (FT-IR) spectra: BAI, DG M, BAI-DG M, and the PM were individually mixed with potassium bromide powder to compress into uniform tablets. An FT-IR spectrometer (Nicolet iS5, Thermo Fisher Scientific, Waltham, MA, USA) was used to record the spectrum of each sample in the region of 400~4000 cm⁻¹.

Ultraviolet-visible (UV/Vis) spectra were acquired using a UV-Vis spectrophotometer, the UV-5300 (Hitachi, Tokyo, Japan). Infrared (IR) spectra were measured on a Bruke Vertex 80 (KBr disks) infrared spectrometer. Optical rotations were recorded in methanol (MeOH) solution on an AUTOPOL1 polarimeter at 28 °C (Rudolph, Wilmington, MA, USA). CD spectra were obtained on a J-810 circular dichroism spectropolarimeter (JASCO Corp., J-810, Tokyo, Japan). Electro-spray ionization mass spectrometry (ESIMS) and high-resolution electrospray ionization mass spectrometry (HRESIMS) analyses were measured on a Waters Xevo TQS and a Waters Xevo G2S Q-TOF LC/MS, respectively. NMR spectra were recorded on a Bruker 600 MHz instrument, and tetramethylsilane (TMS) was used as an internal standard. Column chromatography was performed on silica gel (200−300 mesh, Qingdao Marine Chemical Co., Ltd., Qingdao, China) and Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden), respectively. HPLC analysis was performed on an Agilent 1260 with a C18 column (Agilent Extend-C18, 4.6 × 250 mm, 5 μm, 1 mL/min). HPLC separation was performed on Shimadzu Soviet production LC-16P with an ODS column (Silgreen C18AB, 10 × 250 mm, 5 μm, 180 Å, 3 mL/min). Cell apoptosis and cycle kits were purchased from BD Biosciences (San Jose, CA, USA).