The mice were transfected with the adenovirus vector through specific siJPH2 tail vein injection (Ad-siJPH2). Infected adult cardiomyocytes were incubated with 2 μmol/l fura-2 for 30 min at 37°C, and then washed twice with Tyrode's solution containing 1.8 mmol/l CaCl 2 . We stimulated cells at 1.0 Hz using field stimulation to evoke Ca 2+ transients. Intracellular Ca 2+ levels were determined by calculating the 340/380 nm ratio of fura-2 fluorescence excited at 510 nm using an IonOptix photometry system (PMT-300, USA). The SR-based calcium content in the cells was determined using 10 mM caffeine. Data were collected and analyzed using SignalAverager Software IonWizard 6.6 (IonOptix), with all parameters set to manufacture defaults.
Pmt 300
Manufactured by IonOptix
Sourced in United States
The PMT-300 is a photomultiplier tube (PMT) designed for high-sensitivity light detection in various scientific and research applications. It is capable of converting low-level light signals into electrical signals for analysis and measurement.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pmt 300
1
Adenovirus-Mediated Cardiomyocyte Ca2+ Imaging
2
Measuring Cardiomyocyte Calcium Dynamics
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Infected adult cardiomyocytes were incubated with 2 µmol/L fura-2 for 30 min at 37 ℃, and then were washed twice with Tyrodes solution containing 1.8 mmol/L CaCl 2 . We stimulated cells at 1.0 Hz using eld stimulation to evoke Ca 2+ transient. Levels of intracellular Ca 2+ were determined by calculation of the 340 to 380 nm (340/380) ratio of Fura-2 uorescence excited at 510 nm using an IonOptix photometry system (PMT-300, USA). The SR based calcium content in cells was determined by using 10 mM caffeine. Data were collected and analyzed by using SignalAverager Software IonWizard 6.6 (IonOptix) and with all parameters set to manufacture defaults.
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