The following primary antibodies were used: mouse anti-cmyc clone 9E10, mouse anti-acetylated-α-tubulin clone 6-11B-1, mouse anti-HA (Sigma-Aldrich Co. Ltd.), rabbit anti-GFP (‘Living Colors A.v. Peptide Antibody’) and mouse anti-UBE2E1 (BD Biosciences Inc); rabbit-anti-γ-tubulin and mouse anti-β-actin clone AC-15 (Abcam Ltd.); mouse anti-cyclin D1 clone A-12 (Santa Cruz Biotechnology Inc); rabbit anti-phospho-β-catenin and rabbit anti-β-catenin (Cell Signalling Technology Inc); and mouse anti-mono- and polyubiquitinylated conjugates clone FK2 and rabbit anti-20S proteasome α7 subunit (Enzo Life Sciences Inc). Rabbit anti-MKS1 has been described previously (Dawe et al., 2007 (link); Näthke et al., 1996 (link)). Secondary antibodies were AlexaFluor488-, and AlexaFluor568-conjugated goat anti-mouse IgG and goat anti-rabbit IgG (Molecular Probes Inc) and HRP-conjugated goat anti-mouse immunoglobulins and goat anti-rabbit immunoglobulins (Dako Inc).
Mouse anti cyclin d1 clone a 12
Manufactured by Santa Cruz Biotechnology
Mouse anti-cyclin D1 clone A-12 is a primary antibody that recognizes the cyclin D1 protein in mouse samples. Cyclin D1 is a cell cycle regulatory protein involved in the progression of the cell cycle. This antibody can be used to detect and analyze cyclin D1 expression in various mouse-derived biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti ube2e1, by BD (2 mentions)
Rabbit anti phospho β catenin and rabbit anti β catenin, by Cell Signaling Technology (2 mentions)
Mouse anti mono and polyubiquitinylated conjugates clone fk2 and rabbit anti 20s proteasome α7 subunit, by Enzo Life Sciences (2 mentions)
Mouse anti cmyc clone 9e10, by Merck Group (2 mentions)
Mouse anti ha, by Merck Group (2 mentions)
Mouse anti acetylated α tubulin clone 6 11b 1, by Merck Group (1 mentions)
Mouse anti acetylated tubulin clone 6 11b 1, by Merck Group (1 mentions)
2 protocols using mouse anti cyclin d1 clone a 12
1
Immunoblotting and Immunofluorescence Protocols
2
Antibody Optimization for Protein Detection
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The following primary antibodies were used: mouse anti-cmyc clone 9E10, mouse anti-acetylated--tubulin clone 6-11B-1, mouse anti-HA (Sigma-Aldrich Co. Ltd.), rabbit anti-GFP ("Living Colors A.v. Peptide Antibody") and mouse anti-UBE2E1 (BD Biosciences Inc.); rabbit-anti--tubulin and mouse anti-β-actin clone AC-15 (Abcam Ltd.); mouse anti-cyclin D1 clone A-12 (Santa Cruz Biotechnology Inc.); rabbit anti-phosphoβ-catenin and rabbit anti-β-catenin (Cell Signalling Technology Inc.); and mouse anti-mono-and polyubiquitinylated conjugates clone FK2 and rabbit anti-20S proteasome α7 subunit (Enzo Life Sciences Inc.). Rabbit anti-MKS1 has been described previously 51, (link)52 (link) . Secondary antibodies were AlexaFluor488-, and AlexaFluor568-conjugated goat anti-mouse IgG and goat anti-rabbit IgG (Molecular Probes Inc.) and HRPconjugated goat anti-mouse immunoglobulins and goat anti-rabbit immunoglobulins (Dako Inc.).
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