Vitek 2 ast gn cards

The susceptibility test of all investigated K. pneumoniae isolates was done by determining minimum inhibitory concentrations (MICs) using Vitek 2 AST-GN cards (BioMerieux, France) [9] . The antimicrobial susceptibility was assayed to Gentamicin (4, 16, 32 µg/ml), Amikacin (8, 16, 64 µg/ml), Ciprofloxacin (0.5, 2, 4 µg/ml), and Levofloxacin (0.25, 0.5, 2, 8 µg/ml). Several pure colonies from an overnight culture of K. pneumoniae were transferred to 3 ml normal saline to form a homogenous bacterial suspension with a turbidity range of 0.50-0.63. Then, 145 µl of the suspension was transferred into 3 ml normal saline in a second tube which was placed in a cassette opposite the AST-GN card [9] . The results were interpreted following CLSI [10] .

The antimicrobial susceptibility was tested by determining the minimum inhibitory concentrations (MICs) using Vitek 2 AST-GN cards (BioMerieux, France) by Vitek 2 Compact System [9] . All K. pneumoniae isolates were tested for susceptibility to Ceftazidime (1, 2, 8, 32 µg/ml), Ceftriaxone (1, 2, 8, 32 µg/ml), Cefepime (2, 8, 16, 32 µg/ml), Ertapenem (0.5, 1, 6 µg/ml), Imipenem (1, 2, 6, 12 µg/ml), Ciprofloxacin (0.5, 2, 4 µg/ml), Levofloxacin (0.25, 0.5, 2, 8 µg/ml), Nitrofurantoin (16, 32, 64 µg/ml), Trimethoprime-Sulfamethoxazole (1/19, 4/76, 16/304 µg/ml), and Tigecycline (0.75, 2, 4 µg/ml). Several pure colonies from overnight K. pneumoniae isolates on MacConkey agar were transferred to 3 ml normal saline to form a homogenous bacterial suspension with a turbidity range of 0.50-0.63. Then, 145 µl of the suspension was transferred for AST-GN cards [9] . The results were interpreted following CLSI [10] , except for tigecycline which was approved according to FDA because no CLSI breakpoints are available for this drug [11] .