Lc 1 sq

Cells were incubated at 6% CO 2 and at 37 °C in monolayer culture under humidified conditions. Human lung lines NCI-H520 and NCI-H596, pancreatic line BxPC3, breast line BT-20, colon line HCT116, and liver line HEPG2 were sourced from the American Type Culture Collection (ATCC; Manassas, VA). Lung line LUDLU-1 was purchased from the European Collection of Cell Cultures (Salisbury, UK). SMMC-7721, a liver cell line, was sourced from Shanghai Medicilon, Inc. (Shanghai, China). Human lung lines LC-1/ sq and LK-2 were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). NCI-H520, NCI-H596, LUDLU-1, SMMC-7721, and LK-2 were cultured in RPMI supplemented with 10% fetal bovine serum (FBS; ATCC). BxPC3 was cultured with RPMI with 10% FBS (ATCC), 10 mM HEPES, and 10 mM sodium pyruvate. HEPG2 and BT-20 were maintained with minimum essential media, 10% FBS (ATCC), nonessential amino acids, and 10 mM sodium pyruvate. Lung line LC-1/ sq was grown in a 1:1 RPMI/Ham's F12 medium supplemented with 10% FBS (ATCC). The HCT116 colon line was cultured with McCoy's 5A media with 10% FBS (ATCC). All cell lines tested mycoplasma negative prior to their experimental use. All cell culture media and supplements, except as noted above, were from Life Technologies (Carlsbad, CA).

We purchased the human lung SCC cell lines LK-2, EBC-1, and RERF-LC-AI from Riken Bioresource Center (Tsukuba, Japan) and LC-1sq from the JCRB Cell Bank (Tokyo). The EBC-1 and RERF-LC-AI cells were cultured in MEM (Invitrogen-Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 U/ml streptomycin. The LK-2 cells were cultured in RPMI 1640 (Invitrogen-Gibco) supplemented with 10% FBS, 100 U/ml penicillin and 100 U/ml streptomycin. The LC-1sq cells were cultured with a 1:1 mixture of RPMI 1640 and Ham's F12 (Invitrogen-Gibco) supplemented with 10% FBS, 100 U/ml penicillin and 100 U/ml streptomycin.