Fatty acid methyl esters (FAMEs) were prepared from the extracted lipids by esterification in a methanolic KOH solution (0.500 M, VWR) with the addition of a 20 % BF 3 -MeOH solution (Sigma-Aldrich) according to Joseph and Ackman [33] . The fatty acid composition was determined with a Chrompack CP 9002 equipped with flame ionisation detector, a split/splitless injector, a CTC analytics Liquid Sampler type CTC-A200S (CP9010) and an SLB-IL111 (60 m × 0.25 mm i.d., Sigma-Aldrich) capillary column. The carrier gas was helium at a flow rate of 21 cm/s. The oven temperature was programmed, starting at 140 °C (2 min), increasing to 180 °C (8 °C/min), and subsequently to 260 °C (4°C/min). The fatty acids were identified by comparing their retention times with those of the FAME standards under the same conditions. Methyl pentadecanoate was used as an internal standard. All standards were supplied by Sigma-Aldrich.
Slb il111
Manufactured by Merck Group
Sourced in United States
The SLB-IL111 is a laboratory equipment product manufactured by Merck Group. It is a specialized device designed for use in research and scientific applications. The core function of the SLB-IL111 is to perform precise measurements and analyses, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using slb il111
1
Fatty Acid Methyl Esters Preparation and Analysis
2
Identification and Quantification of c-20:1 Fatty Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each FAME sample was screened for the availability of c-20:1 by analyzing total FA composition using GC-2014 (Shimadzu Corporation) fitted with a flame ionization detector (FID) . FAME separation was carried out on the Omegawax 320 capillary GC column (30 m×0.32 mm×0.25 μm, Sigma-Aldrich Japan K.K.) and peaks were identified using the Supelco 37 component FAME mix standard (data for total FA composition are not shown) . The c-20:1 FAME fractions obtained from HPLC were subjected to GC-FID analysis using GC-2014 (Shimadzu Corporation) fitted with a highly polar IL capillary column (SLB-IL111, 100 m×0.25 mm×0.2 μm, Sigma-Aldrich Japan K.K.) . Injector and detector temperatures were maintained at 250℃. Separation was carried out isothermally at 160℃ using helium (1.2 mL/min) as the carrier gas. The split ratio was 100:1. Three replicate analyses (from step 2.3 to step 2.5) were performed per sample.
3
Gas Chromatographic Analysis of Fatty Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Extraction of FA was performed according to the boron trifluoride method described by Yurchenko et al. (2016) . Fatty acid methyl esters were analyzed on a Varian 3900 gas chromatograph equipped with a flame ionization detector and autosampler CP-8400. Chromatographic separation of FAME was performed using a Supelco ionic liquid column SLB-IL111 (100m × 0.25 mm i.d., 0.20 µm film thickness). Tridecanoic acid methyl ester (C13:0) as internal standard and CRM47885 standard mixture as external standard were used. A more detailed method description has been described by Yurchenko et al. (2018) (link).
The following sums and indices were calculated: SFA, MUFA, PUFA, atherogenic index, desaturase index (DI), and ratio of n-6 and n-3 FA (n-6/n-3). The atherogenic index was calculated as has been proposed by Ulbricht and Southgate (1991) (link) as follows: atherogenic index = (C12:0 + 4 × C14:0 + C16:0)/(MUFA + n-6 + n-3), and n-6/n-3 as n-6/n-3 = (C18: 2n -6 + C20: 2n -6 + C18: 3n -6)/C18: 3n -3. The DI was defined as follows: product of desaturase/substrate of desaturase and calculated for 3 pairs of FA (DI14 = C14:1 cis-5/C14:0; DI16 = C16:1 cis-7/C16:0; DI18 = C18:1 cis-9/C18:0).
The following sums and indices were calculated: SFA, MUFA, PUFA, atherogenic index, desaturase index (DI), and ratio of n-6 and n-3 FA (n-6/n-3). The atherogenic index was calculated as has been proposed by Ulbricht and Southgate (1991) (link) as follows: atherogenic index = (C12:0 + 4 × C14:0 + C16:0)/(MUFA + n-6 + n-3), and n-6/n-3 as n-6/n-3 = (C18: 2n -6 + C20: 2n -6 + C18: 3n -6)/C18: 3n -3. The DI was defined as follows: product of desaturase/substrate of desaturase and calculated for 3 pairs of FA (DI14 = C14:1 cis-5/C14:0; DI16 = C16:1 cis-7/C16:0; DI18 = C18:1 cis-9/C18:0).
4
Milk Fatty Acid Profiling by GC-FID
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FP and PP were measured in milk samples with MilkoScan FT 6000 Series mid-range infrared Fourier transform infrared-based spectrometers (Foss, Hillerod, Denmark) by Valacta, the Dairy Production Centre of Expertise for Quebec and the Atlantic provinces (Valacta Laboratories Inc., Ste-Anne-de-Bellevue, QC, Canada, www.valacta.com ). Individual FA profiles were determined by gas chromatography according to O’Fallon et al. [41 (link)]. The Hewlett Packard 6890 N gas chromatographic system (Agilent Technology, Wilmington, DE, USA) equipped with a flame ionization detector and an auto sampler (Hewlett Packard, Avondale, PA, USA) and the gas chromatographic capillary column SLB-IL111 (100 m × 0.25 mm, 0.2 μm in thickness, Supelco, Bellefonte, PA, USA) were used. Hydrogen was the gas carrier at 1 mL/min constant flow with linear velocity of 26 cm/s. Oven temperature was set at 40 °C for 1 min, followed by 80 °C to 170 °C for 1 min, 40 °C to 195 °C for 2 min and 20 °C to 210 °C for 15 min. Injection port and detector temperatures were set at 250 °C, split ratio was set to 1:100 and injection volume was 1 μl. Quantification of FAs was performed with Agilent Technologies Chemstation vB.04.03 software using the FA methyl ester standard GLC No. 463 (Nu-Chek Prep Inc., Elysian, MN, USA) and C13:0 as the internal standard.
5
Fatty Acid Profiling via GC-FID
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fatty acid methyl ester preparation and quantification of fatty acid profiles have been reported previously [11 (link)]. Briefly, fatty acid methyl esters were prepared and quantified using the Hewlett Packard 6890 N gas chromatographic system (Agilent Technology, Wilmington, DE, USA). The carrier gas was hydrogen and the capillary column used was SLB-IL111 (100 m × 0.25 mm, 0.2 µm in thickness, Supelco, Bellefonte, PA, USA). The oven temperature was set at 40 °C for 1 min followed by 80 °C to 170 °C for 1 min, then 40 °C to 195 °C for 2 min and finally 20 °C to 210 °C for 15 min. Individual fatty acids were determined by comparing their retention time with that of fatty acid methyl esters standards (GLC No. 463 and No. UC-59-M, Nu-Chek Prep Inc., Elysian, MN, USA). The Chemstation B.04.03 software (Agilent technologies) was used for data analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!