Normal human colon mucosal epithelial (FHC) cells and HT29, HCT116, SW480, and SW620 colon cancer cells were purchased from Nanjing KeyGen Biotech Co., Ltd., China. The FHC cells were cultured in RPMI 1640 medium (Gibco, USA) supplemented with 10% foetal bovine serum (FBS, Biological Industries, Israel) and penicillin/streptomycin solution. The HT29 and HCT116 cells were cultured in McCoy’s 5A medium (Keygen Biotech) supplemented with 10% FBS. The SW480 and SW620 cells were cultured in L15 medium (KeyGen Biotech) containing 10% FBS. All the cells were cultured in a cell culture incubator at 37 °C with 5% CO2. All the cells were identified within the past year based on short tandem repeats (STRs) or single nucleotide polymorphisms (SNPs) and were not contaminated with mycoplasma.
Sw480
Manufactured by Keygen Biotech
Sourced in China
The SW480 is a laboratory equipment used for cell culture and analysis. It is a human colorectal adenocarcinoma cell line derived from a primary tumor. The SW480 cell line is commonly used in cancer research and drug development studies.
Automatically generated - may contain errors
Lab products found in correlation
Hct116, by Keygen Biotech (17 mentions)
Sw620, by Keygen Biotech (17 mentions)
Hct 8, by Keygen Biotech (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Mccoy s 5a medium, by Keygen Biotech (6 mentions)
Ncm460, by Keygen Biotech (5 mentions)
L15 medium, by Keygen Biotech (5 mentions)
Fetal bovine serum (fbs), by Sartorius (5 mentions)
Sw480, by Thermo Fisher Scientific (4 mentions)
Sw1116, by Keygen Biotech (3 mentions)
Sw620, by Thermo Fisher Scientific (3 mentions)
Sw480, by Wuhan Boster Biological Technology (3 mentions)
Hct116, by Wuhan Boster Biological Technology (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Leibovitz s l 15, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Ncm460, by Wuhan Boster Biological Technology (2 mentions)
Sw620, by Wuhan Boster Biological Technology (2 mentions)
Caco 2, by Keygen Biotech (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
24 protocols using sw480
1
Colon Cancer Cell Lines and Culture
2
Macrophage Polarization and CRC Cell Phenotypes
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Human THP-1 macrophages were stationarily cultured with RPMI-1640 medium (brand: Gibco, item No.: C11875500BT) containing 10% FBS (Gibco, 10099-141), 10 U/mL penicillin and 10 μg/mL streptomycin in a 5% CO2 incubator at 37°C. In the culture process, the cell count was kept at 1×107/culture flask. Next, the sub-cultured THP-1 cells were diluted into 1×106/mL, inoculated in a 35 mm culture dish and cultured in low-serum RPMI-1640 medium containing 100 ng/mL phorbol-12-myristate-13-acetate (PMA) (MCE, HY-18739) and 0.3% BSA to induce their differentiation into M0 macrophages. Furthermore, 10 μm of SHP-2 inhibitors PHPS1 (brand: MCE, HY-112368) and/or 15 ng/mL IL-4 (MCE, HY-P70445) and/or 30 μM of PI3K inhibitors LY294002 (MCE, HY-10108) was added into the medium and incubated for 24 hours. According to experimental requirements, the treated cells were divided into control group, PHPS1 group, IL-4 group, PHPS1+IL-4 group, IL-4+ LY294002 group and PHPS1+IL-4+LY294002 group.
CRC cell lines SW480 and HCT116 were purchased from Nanjing KeyGen BioTech Co., Ltd. After being intervened by PHPS1 and/or IL-4 for 24 hours, macrophages were centrifuged and co-cultured with CRC cell lines SW480 and HCT116. Then, the phenotypes of CRC cells were experimentally observed.
CRC cell lines SW480 and HCT116 were purchased from Nanjing KeyGen BioTech Co., Ltd. After being intervened by PHPS1 and/or IL-4 for 24 hours, macrophages were centrifuged and co-cultured with CRC cell lines SW480 and HCT116. Then, the phenotypes of CRC cells were experimentally observed.
3
Colorectal Cancer Cell Line Culturing
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CRC cell lines HT-29, CT-26, SW480, SW620, HT-29, HCT-116, SW1116 and normal colorectal epithelial cell line NCM460 were purchased from Keygen (Nanjing, China); Oxaliplatin resistant HCT-116-OR cells were purchased from Shanghai Meixuan Biological Technology Co., LTD. Cell lines were passaged in our laboratory. Cell line authentication was assessed using short tandem repeat (STR) DNA profiling method every 6 months. All of these cell lines were cultured in RPMI-1640 medium (Keygen) with 10% Fetal bovine serum (FBS, Biological Industries, Kibbutz Beit Haemek, Israel) at 37°C plus 5% CO2. Recombinant human TGF beta Receptor II (rTGFBR2) protein was purchased from Abcam (Cambridge, MA, USA) and 50 nM was used in this study.
4
Cell Culture Conditions for CRC Lines
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Human CRC cells SW620, HCT116, SW480, HT29, and normal colonic epithelia cells (NCM460) were purchased from Boster Company (Boster, Wuhan, People’s Republic of China). SW620 and SW480 were grown in L15 medium (KeyGEN BioTECH, Nanjing, China) supplemented with 10% FBS (Biological Industries, Israel). HCT116, HT29, and NCM460 cells were grown in McCoy′s5A medium (KeyGEN BioTECH, Nanjing, China) supplemented with 10% FBS (Biological Industries, Israel). All cells were incubated at 37 °C with 5% CO2.
5
Characterizing Colorectal Cancer Cell Lines
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The human CRC cell lines SW620, SW480, HCT-8, 5-FU-resistant HCT-8 cells (HCT-8/5-FU) and LoVo were obtained from KeygenBiotech Co. Ltd. (Nanjing, China), which was recently authenticated as truly CRC cell lines. SW620 and SW480 were cultured in Leibovitz’s L-15 (Gibco, Grand Island, NY) medium contained 10% in activated fetal bovine serum (Gibco, Grand Island, NY). 5-FU was added to LoVo cell culture in stepwise increasing concentrations for over 6 months, namely LoVo/5-FU. HCT-8/5-FU and LoVo/5-FU were maintained in RPMI1640 supplementd with 114.7 μM and 107.0 μM 5-FU, respectively. CRC cells were incubated at 37 °C containing 5%CO2. All cells lines were routinely tested for mycoplasma, which were shown to be negative.
6
Cell Culture Conditions for Colorectal Cancer Research
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Human normal colorectal epithelial cell line (FHC) and CRC cell line, including SW480 and SW620, cells were obtained from KeyGEN Company (Nanjing city, Jiangsu Province, China). Human embryonic kidney cell line (HEK293T) cells and umbilical vein endothelial cells (HUVECs) were obtained from the Institute of Biochemistry (Shanghai, China). FHC cells, HEK293T cells and HUVECs were cultured in 90% DMEM (Gibco) supplemented with antibiotics (1 × penicillin/streptomycin100 U/ml, Gibco) and 10% heat-inactivated foetal bovine serum (FBS) (Gibco, Grand Island, NY, USA). SW480 and SW620 cells were cultured in 90% L-15 (Gibco) supplemented with antibiotics and 10% FBS. The cells were incubated at 37 °C in a humidified and 5% CO2 incubator.
7
Cell Line Culturing and Maintenance
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The DLD-1 CRC cell line was purchased from Procell Life Science & Technology Co., Ltd., and the RKO, SW480, NCM460 and HCT116 cell line from Nanjing KeyGen Biotech Co., Ltd. All cell lines were STR-authenticated. These cells were incubated at 37 °C, 5% CO2, McCoy’s 5A or DMEM medium (Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (Gibco, USA) for HCT116 cells and the rest cell lines, respectively. The medium was renewed every 3 days and the cells were passaged when the cell density reached 80–90%.
8
Culturing Human Colon Cell Lines
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The human normal colonic epithelial cell line (FHC) was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). Human CRC cell lines (HT29, RKO, HCT116, SW480, SW620, LoVo, HCT8 and LS123) were obtained from KeyGEN BioTECH (Nanjing, Jiangsu, China). The cell lines were cultured in appropriate medium supplemented with 10% fetal bovine serum (FBS; Biological Industries, Israel) and 1% antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin; Life Technologies, Inc., Grand Island, NY, USA). All cell lines were maintained in an incubator at 37°C in a humidified atmosphere with 5% CO2. All cell experiments were performed using mycoplasma-free cells.
9
Cell Culture Conditions for Human Colorectal Cancer
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Human CRC cell lines (SW480, SW620, HCT116, HT29) and normal colonic epithelial cell lines (NCM460) were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China). SW480 and SW620 cells were cultured in L15 medium (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) containing 10% fetal bovine serum (FBS: Biological Industries Israel Beit-Haemek, Beit-Haemek, Israel). HCT116 and HT29 were cultured with McCoy's 5A medium (Nanjing KeyGen Biotech Co., Ltd.) containing 10% FBS. NCM460 was cultured in Dulbecco's Modified Eagle Medium (DMEM, Thermo Fisher Scientific™, Beijing China) containing 10% FBS. All cells were incubated at 37 °C in a 5% CO2 incubator.
10
Culturing Colorectal Cancer Cell Lines
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Human normal colonic epithelial cell line HCoEpic and four CRC cell lines, including SW620, SW480, HT29, and HCT116, were purchased from Nanjing KeyGen Biotech. All the cell lines were stored in liquid nitrogen and recovered in complete DMEM medium (Thermo, China) before use. Then, these cells were cultured in humidified chamber with 5% carbon dioxide at 37 °C.
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