Rabbit anti βiii tubulin tuj 1

Manufactured by Fortrea

Rabbit anti-βIII-tubulin/Tuj 1 is a laboratory reagent used for the detection and analysis of the βIII-tubulin protein, also known as Tuj 1. βIII-tubulin is a specific marker for neurons and is widely used in the study of neuronal development and function.

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Rabbit anti-βIII-tubulin Anti-βIII-tubulin βIII-Tubulin Anti-Tubulin

2 protocols using rabbit anti βiii tubulin tuj 1

Immunofluorescence Staining of Stem Cells

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Human cells were fixed in 4% paraformaldehyde in PBS at 4 °C for 10 min, permeabilized at room temperature for 15 min in 0.1% Triton in PBS and then blocked in 5% donkey serum with 0.1% Triton at room temperature for 30 min. The following primary antibodies and dilutions were used: goat anti-NANOG (R&D), 1:200; mouse anti-TRA1-60 (Chemicon), 1:100; mouse anti-human NESTIN (Chemicon), goat anti-SOX2 (Santa Cruz), 1:200; rabbit anti-βIII-tubulin/Tuj 1 (Covance), 1:200; mouse anti-MAP2AB (Sigma), 1:200. Secondary antibodies used include Alexa donkey 488 and 568 anti-rabbit (Life Technologies), Alexa donkey 488 and 568 anti-mouse (Life Technologies), and Alexa donkey 488 and 568 anti-goat (Life Technologies); all were used at 1:300. To visualize nuclei, slides were stained with 0.5 μg ml−1 DAPI (4′,6-diamidino-2-phenylindole) and then mounted with Vectashield.

Fulton S.L., Wenderski W., Lepack A.E., Eagle A.L., Fanutza T., Bastle R.M., Ramakrishnan A., Hays E.C., Neal A., Bendl J., Farrelly L.A., Al-Kachak A., Lyu Y., Cetin B., Chan J.C., Tran T.N., Neve R.L., Roper R.J., Brennand K.J., Roussos P., Schimenti J.C., Friedman A.K., Shen L., Blitzer R.D., Robison A.J., Crabtree G.R, & Maze I. (2022). Rescue of deficits by Brwd1 copy number restoration in the Ts65Dn mouse model of Down syndrome. Nature Communications, 13, 6384.

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Neuronal Differentiation and Characterization

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REST siRNA (siREST) (ID #: S11933), non-targeting, negative siRNA (siNEG) (Catalog #: AM4635) and nuclease-free water were purchased from Ambion. The transfection reagent, TransIT-TKO, was obtained from Mirusbio. Diethylpyrocarbonate treated Tris-EDTA buffer (DEPC-treated TE Buffer) was purchased from 1st Base, Singapore. M-MLV Reverse transcriptase (M3681) was purchased from Promega. iQ SYBR Green Supermix was purchased from Bio-Rad. 2, 2, 2-trifluoroethanol (TFE) was purchased from Acros Organics.
Polycaprolactone (PCL, Mw:70000-90000), 3,4-Dihydroxy-L-phenylalanine (DOPA), chloroform, 10% formalin, tris-buffered saline (TBS), Triton X-100, fluoromount, bovine serum, albumin (BSA) were purchased from Sigma-Aldrich. Fetal bovine serum (FBS) was obtained from Hyclone. Goat anti-nestin was purchased from Santa Cruz. Rabbit antimicrotubule associated protein-2 (MAP2), chicken anti-glial fibrillary acidic protein (GFAP), mouse anti-O4, anti-Olig2 and anti-ALDH1L1 were purchased from Millipore. Rabbit anti-βIII tubulin (Tuj1) was purchased from Covance. All other reagents were purchased from Life Technologies.

Low W.C., Rujitanaroj P.O., Lee D.K., Kuang J., Messersmith P.B., Chan J.K, & Chew S.Y. (2015). Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment. Macromolecular bioscience, 15(10).

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