47885 u supelco

The fatty acid composition of lipids was determined according to the method described by Lurueña-Martínez et al. [32 (link)] in the samples taken on the 21st day of the ripening. Intramuscular lipids were extracted by using the chloroform/methanol procedure [33 (link)]. Extracted fatty acids were methylated with 0.2 KOH in anhydrous methanol and subsequently analyzed by gas chromatography (GC 6890 N, Agilent Technologies, Santa Clara, CA, USA) using a 100 m × 0.25 mm × 0.20 µm fused silica capillary column (SP-2560, Supelco, Inc, Bellefonte, PA, USA). In total, 1 µL was injected into the chromatograph, which was equipped with a split/splitless injector and a flame ionization detector (FID). The oven temperature program was started at 150 °C followed by increases of 1.50 °C/min up to 225 °C, at which point it was maintained for 15 min. The temperature of the injector and detector was 250 °C. The carrier gas was helium at 1 mL/min and the split ratio was 20:1. The different fatty acids were identified by their retention times using a mixture of fatty acid standards (47885-U Supelco, Sigma-Aldrich, Darmstadt, Germany). Fatty acid contents were calculated by using chromatogram peak areas and were expressed as g per 100 g of total fatty acid methyl esters. All analyses were performed in duplicate.

Intramuscular lipids were extracted using the chloroform/methanol procedure described by Folch et al. [36 (link)]. The fatty acid composition of lipids was determined according to the method described by Lurueña-Martínez et al. [37 (link)]. Extracted fatty acids were methylated with KOH 0.2 M in anhydrous methanol and then analyzed via gas chromatography (GC 6890 N, Agilent Technologies, Santa Clara, CA, USA) using a 100 m × 0.25 mm × 0.20 µm fused silica capillary column (SP-2560, Supelco, Inc, Bellefonte, PA, USA). One microliter was injected into the chromatograph, which was equipped with a split/splitless injector and a flame ionization detector (FID). The oven temperature program was started at 150 °C, followed by increases of 1.50 °C/min up to 225 °C, at which point it was maintained for 15 min. The temperature of the injector and detector was 250 °C. The carrier gas was helium at 1 mL/min and the split ratio was 20:1. The different fatty acids were identified by the retention time using a mixture of fatty acid standards (47885-U Supelco, Sigma-Aldrich, Darmstadt, Germany). The fatty acid contents were calculated using chromatogram peak areas and were expressed as g per 100 g of total fatty acid methyl esters. All analyses were performed in triplicate.