V plex aβ peptide panel 1 6e10

Quantitative biochemical analyses of human Aβ and inflammatory cytokines in mouse tissue were performed using a commercially available electrochemiluminescence-linked immunoassay from Meso Scale Discovery (MSD, Gaithersburg, MD, USA). The V-PLEX Aβ Peptide Panel 1 (6E10) was used and plates were analyzed on the MS2400 imager (MSD). Assays were performed according to the manufacturer’s instructions, and all standards and samples were measured in duplicate.

A number of biomarkers were measured in CSF and blood plasma. CSF biomarkers were obtained by lumbar puncture performed in the morning after fasting by one of the authors (JCA). Blood draws were performed during the same patient visit. Samples were centrifuged, aliquoted, and stored at −80°C for later analysis.
Levels of CSF Tau protein phosphorylated at threonine position 181 (pTau) were measured using the Innotest Phospho-Tau (181P) ELISA (Fujirebio US; Malvern PA). Prior to analysis, all CSF underwent one freeze-thaw cycle. Assays were performed according to manufacturer protocols and were read with a Bio-Tek multimodal plate reader with absorbance at 450 nm. The output data were used to quantify the concentrations based on the supplied in-assay standard curve. We measured β-amyloid₁₋₄₂ (Aβ₁₋₄₂) and β-amyloid₁₋₄₀ (Aβ₁₋₄₀) to calculate the Aβ₁₋₄₂/Aβ₁₋₄₀ ratio (V-PLEX Aβ Peptide Panel 1–6E10; MesoScale Discovery MSD, Rockville, Maryland). The output data were used to quantify the concentrations based on the 2-fold sample dilation and the supplied in-assay standard curve. All data were expressed as pg/mL, though the ratio is unitless.