Plasmodium falciparum 3D7 (American Type Culture Collection, ATCC PRA-405D) and P. falciparum FCR3 (American Type Culture Collection, ATCC® 30932) were purchased from the ATCC (Manassas, USA). The chloroquine-susceptible strain Plasmodium berghei NK65 (MRA-268) and the atovaquone-resistant strain P. berghei NAT (MRA-415) were purchased from Bei Resources (Manassas, USA). The P. falciparum strains 3D7 and FCR3 were grown in human erythrocytes as previously described [27 (link)]. Briefly, parasites were maintained in continuous culture with 5% haematocrit of type O human red blood cells suspended in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 24 mM NaHCO3, 25 mM HEPES, 0.8% hypoxanthine, 0.9% Albumax, and 25 μg/mL of gentamicin. The 6-well plates were placed in an incubator (atmosphere: 5% CO2, 5% O2, and 90% N2) at 37 °C and the medium was changed daily when the level of parasitaemia was at least 5%. The parasite density was determined by Giemsa staining of thin smears and expressed as a percentage of infected erythrocytes in a field of a total of 500 erythrocytes.
Atcc pra 405d
Manufactured by American Type Culture Collection
Sourced in United States
The ATCC PRA-405D is a laboratory equipment product designed for culturing cells. It provides a controlled environment for the growth and maintenance of cell lines. The product's core function is to facilitate the cultivation of cells under specific temperature, humidity, and gas composition settings.
Automatically generated - may contain errors
Lab products found in correlation
Dihydroartemisinin dha, by Merck Group (1 mentions)
Sytox green, by BD (1 mentions)
Differential quik 3 stain kit, by Polysciences (1 mentions)
Chloroquine cq, by Merck Group (1 mentions)
Propidium iodide, by BD (1 mentions)
Vero cell, by American Type Culture Collection (1 mentions)
Hypoxanthine, by Merck Group (1 mentions)
Gentamicin, by Thermo Fisher Scientific (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Differential quick 3 stain kit, by Polysciences (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
3 protocols using atcc pra 405d
1
Culturing Plasmodium falciparum and Plasmodium berghei
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Plasmodium falciparum 3D7 (American Type Culture Collection, ATCC PRA-405D) and P. falciparum FCR3 (American Type Culture Collection, ATCC® 30932) were purchased from the ATCC (Manassas, USA). The chloroquine-susceptible strain Plasmodium berghei NK65 (MRA-268) and the atovaquone-resistant strain P. berghei NAT (MRA-415) were purchased from Bei Resources (Manassas, USA). The P. falciparum strains 3D7 and FCR3 were grown in human erythrocytes as previously described [27 (link)]. Briefly, parasites were maintained in continuous culture with 5% haematocrit of type O human red blood cells suspended in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 24 mM NaHCO3, 25 mM HEPES, 0.8% hypoxanthine, 0.9% Albumax, and 25 μg/mL of gentamicin. The 6-well plates were placed in an incubator (atmosphere: 5% CO2, 5% O2, and 90% N2) at 37 °C and the medium was changed daily when the level of parasitaemia was at least 5%. The parasite density was determined by Giemsa staining of thin smears and expressed as a percentage of infected erythrocytes in a field of a total of 500 erythrocytes.
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Plasmodium falciparum 3D7 (American Type Culture Collection, ATCC PRA-405D) and P. falciparum FCR3 (American Type Culture Collection, ATCC® 30932) were purchased from the ATCC (Manassas, USA). The chloroquine-susceptible strain Plasmodium berghei NK65 (MRA-268) and the atovaquone-resistant strain P. berghei NAT (MRA-415) were purchased from Bei Resources (Manassas, USA). The P. falciparum strains 3D7 and FCR3 were grown in human erythrocytes as previously described [27 (link)]. Briefly, parasites were maintained in continuous culture with 5% haematocrit of type O human red blood cells suspended in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 24 mM NaHCO3, 25 mM HEPES, 0.8% hypoxanthine, 0.9% Albumax, and 25 μg/mL of gentamicin. The 6-well plates were placed in an incubator (atmosphere: 5% CO2, 5% O2, and 90% N2) at 37 °C and the medium was changed daily when the level of parasitaemia was at least 5%. The parasite density was determined by Giemsa staining of thin smears and expressed as a percentage of infected erythrocytes in a field of a total of 500 erythrocytes.
2
Antimalarial Combination Therapy Evaluation
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Chloroquine (CQ), Atovaquine, and Dihydroartemisinin (DHA) were from Sigma-Aldrich (St. Louis, MO, USA). The Differential Quik III Stain Kit was obtained from Polysciences Inc. (Valley Road Warrington, PA, USA). Propidium iodide (PI) and SYTOX Green solution were brought from BD Pharmingen, Inc. (San Diego, CA, USA) and Life Technologies (Grand Island, NY, USA), respectively. The CQ-sensitive strain Plasmodium falciparum (3D7) (American Type Culture Collection, ATCC PRA-405D) and Vero cells were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA). The Atovaquone-resistant rodent malaria strain Plasmodium berghei NAT (MRA-415), was obtained from BEI Resources (Manassas, VA, USA).
3
Antimalarial Drug Screening Protocol
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Chloroquine, sodium bicarbonate, and hypoxanthine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gentamicin, SYTOX green solution, AlbuMAX I Lipid-Rich bovine serum albumin, and RPMI 1640 medium supplemented with 25 mM HEPES and L-glutamine were purchased from Gibco (Life Technologies Corporation, Grand Island, NY, USA). The Differential Quick III Stain Kit was purchased from Polysciences Inc. (Valley Road, Warrington, PA, USA). CQ-sensitive strain P. falciparum (3D7) (American Type Culture Collection, ATCC PRA-405D) and Vero cells (CRL-1587) were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA). A multiple antimalarial drug-resistant strain, P. falciparum K1 (MRA-159), and a CQ-susceptible strain, P. berghei NK65 (MRA-268), were obtained from BEI Resources (Manassas, VA, USA) [11 (link)].
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