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2 protocols using cyclophosphamide for injection

1

Cyclophosphamide-based Lipidomics Protocol

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Cyclophosphamide for injection (batch number 9B285A) from Baxter Oncology GmbH, Halle, Germany; internal standard, lyso PE (17:1) (batch number: LM171LPE-11), SM (17:0) (batch number: 170SM-13), PE (17:0/17:0) (batch number: LM170PE-19) from Avanti Polar Lipids, Alabaster, AL, USA; methyl tert-butyl ether (MTBE), N-hexane, isopropanol, ammonium acetate and ammonium formate (99.8% mass spectrometry pure) from ROE Scientific, Newark, NJ, USA; glacial acetic acid (batch number 15080553613, purity: 99.5%) from Nanjing Chemical Reagent Co., Ltd., Nanjing, China; acetonitrile and methanol (99.8% mass spectrometry pure) from Merck, Darmstadt, Germany; methoxyamine hydrochloride, pyridine, N, O-Bis (trimethylsilyl) triflfluoroacetamide (BSTFA, batch number 15238) with 1% trimethylchlorosilane (TMCS), formic acid and 1,2-13C-myristic acid from Sigma Aldrich, St. Louis, MO, USA; methanol from Supelco, Bellefonte, PA, USA; and malonaldehyde (MDA) and superoxide dismutase (SOD) activity detection kit from Wuhan Abbkine Company, Wuhan, China.
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2

Maternal exposure effects on neonatal rats

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(±)-Epinephrine HCl, (L)-norepinephrine bitartrate, (R)-phenylephrine HCl, sodium pentobarbital, N-nitroso-N-methylurea (MNU), resveratrol, Nile Red dye, spermine and solvents for drug delivery were all obtained from Sigma (St. Louis, MO). L-norepinephrine–HCl was obtained from ChemPacific (Baltimore, MD). Cyclophosphamide for injection was from Baxter (Deerfield, IL). Coal Black dye (#5006) was from Byrnes & Kiefer (Evans City, PA). The PrC-210 aminothiol was synthesized as described previously.18 Lactating female Sprague–Dawley rats with litters of 10-day-old pups were obtained from Harlan (Indianapolis, IN). Rats were maintained on 12-hr light/dark cycle and provided ad lib water and lab chow. All animal procedures were conducted according to a protocol (#M0476) approved by the University of Wisconsin Institutional Animal Care and Use Committee.
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