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Bergamottin

Manufactured by Merck Group
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Bergamottin is a naturally occurring furanoflavonoid compound found in certain citrus fruits, such as bergamot. It serves as a key ingredient in various pharmaceutical and biochemical applications, particularly in the field of analytical chemistry. Bergamottin is utilized as a reference standard and in the development of analytical methods for the identification and quantification of related compounds.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Mibefradil, by Bio-Techne (1 mentions) Tangeretin, by Merck Group (1 mentions) Nobiletin, by Merck Group (1 mentions) Hesperidin, by Merck Group (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) D glucose, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) D glucose, by Merck Group (1 mentions) Naringin, by Merck Group (1 mentions) Escherichia coli lps, by Merck Group (1 mentions) Streptomycin solution, by Thermo Fisher Scientific (1 mentions) Omeprazole, by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Furafylline, by Merck Group (1 mentions) Ketoconazole, by Merck Group (1 mentions) β naphthoflavone, by Merck Group (1 mentions)

5 protocols using bergamottin

1

Bergamottin Modulates LPS-Induced Inflammation

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RAW264.7 cells were cultured in DMEM supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA, USA), penicillin (100 IU/ml), and streptomycin (100 μg/ml). To induce inflammatory responses, RAW264.7 cells were exposed to Escherichia coli LPS (100 ng/ml; Sigma-Aldrich, St Louis, MO, USA). Twenty-four h later, the cells and culture media were harvested and tested for gene expression. To evaluate the activity of bergamottin, RAW264.7 cells were pre-treated with bergamottin (20 and 50 μM; Sigma-Aldrich) 12 h before LPS challenge.
An N., Yang T., Zhang X.X, & Xu M.X. (2021). Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB. Innate Immunity, 27(7-8), 543-552.
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2

Obtaining Mibefradil, Azamulin, and Bergamottin

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Mibefradil was obtained from Tocris Bioscience (Minneapolis, MN, USA) azamulin and DHB from Cayman Chemical (Ann Arbor, MI, USA), and bergamottin from Sigma-Aldrich (St. Louis, MO, USA).
, & Sevrioukova I.F. (2019). Structural Insights into the Interaction of Cytochrome P450 3A4 with Suicide Substrates: Mibefradil, Azamulin and 6′,7′-Dihydroxybergamottin. International Journal of Molecular Sciences, 20(17), 4245.
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3

Uptake and Transport of Estrone 3-Sulfate

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[3H]Estrone 3-sulfate ammonium salt (54.3 Ci/mmol, purity >97%) was purchased from Perkin Elmer (Waltham, MA). Estrone 3-sulfate potassium salt, D-glucose, 6′,7′-dihydroxybergamottin (DHB), bergamottin, naringin, hesperidin, tangeretin, and nobiletin were purchased from Sigma-Aldrich (St. Louis, MO). Hanks’ balanced salt solution with calcium and magnesium was purchased from Mediatech Inc. (Hendon, VA). Phosphate-buffered saline (PBS); fetal bovine serum; trypsin-EDTA; HEPES; and Dulbecco’s Modified Eagle Medium (DMEM) containing 4.5 g/L D-glucose, 2 mM L-glutamine, and 110 mg/L sodium pyruvate were purchased from Invitrogen (Carlsbad, CA). MDCKII parental cells and stably transfected MDCKII-OATP2B1 cells were kindly provided by Dr. Markus Grube (Ernst-Moritz-Arndt University, Greifswald, Germany). All other chemicals and reagents were purchased from Fisher Scientific (Pittsburgh, PA). Ethical approval was not required for the following in vitro and in silico research activities.
Johnson E.J., Won C.S., Köck K, & Paine M.F. (2017). Prioritizing pharmacokinetic drug interaction precipitants in natural products: application to OATP inhibitors in grapefruit juice. Biopharmaceutics & drug disposition, 38(3), 251-259.
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4

In Vitro Enzyme Expression Assay

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Minimal essential medium (MEM), fetal bovine serum (FBS), penicillin and streptomycin solution, and 0.25% (v/v) trypsin-EDTA were purchased from Gibco (Carlsbad, CA). Sodium pyruvate, sodium bicarbonate, and nonessential amino acids were purchased from Sigma-Aldrich (St. Louis, MO). Furafylline, β-naphthoflavone, omeprazole, dexamethasone, ketoconazole, dimethylsulfoxide (DMSO), methanol, and natural compounds such as andrographolide, bergamottin, curcumin, lycopene, and resveratrol were purchased from Sigma-Aldrich. The iScript™ One-Step RT-PCR Kit With SYBR® Green was purchased from Bio-Rad Laboratories (Hercules, CA), QIAshredder™, RNeasy® Mini Kit, QuantiFast® Multiplex RT-PCR kit, HotStarTaq® Plus DNA polymerase, and dNTPs mix was obtained from Qiagen (Venlo, Limburg, Netherlands). Primary antibodies against CYP1A2, CYP2D6, and CYP3A4 were bought from Millipore (Billerica, MA) while the primary antibody for β-actin and secondary antibody (anti-rabbit IgG, HRP-linked) was obtained from Cell Signaling Technology (Beverly, MA). Primers were synthesized by Bioneer (Daejeon, Korea) and probes were synthesized by Sigma-Aldrich.
Koe X.F., Tengku Muhammad T.S., Chong A.S., Wahab H.A, & Tan M.L. (2014). Cytochrome P450 induction properties of food and herbal-derived compounds using a novel multiplex RT-qPCR in vitro assay, a drug–food interaction prediction tool. Food Science & Nutrition, 2(5), 500-520.
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5

Furocoumarin-Rich Plant Protocols

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Four plant species, known for their high content of furocoumarins were chosen for experiments; their brief description is presented in Table 5. Grapefruit pomace was produced from fresh fruits and dried on a laboratory scale, while other products were acquired from local sellers.
Analytical grade: psoralen, isopsoralen, methoxsalen, bergapten, osthole, and imperatorin were acquired from Chromadex (Irvine, CA, USA), 6′,7′-dihydroxybergamottin (DHB) came from Toronto Research Chemicals (North York, ON, Canada), and bergamottin was from Sigma-Aldrich (Saint Louis, MO, USA). HPLC-grade methanol, acetonitrile, and tetrahydrofuran (THF) were bought from POCh (Gliwice, Poland). Technical grade carbon dioxide was acquired from a local supplier (Multax, Zielonki-Parcela, Poland).
Woźniak Ł., Połaska M., Marszałek K, & Skąpska S. (2020). Photosensitizing Furocoumarins: Content in Plant Matrices and Kinetics of Supercritical Carbon Dioxide Extraction. Molecules, 25(17), 3805.
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