Poly ethylene glycol methyl ether methacrylate

Similar to our previous work [12 (link),27 (link)], stamp-molding materials for OSTE+ microwell arrays (i.e., UV initiator 1-hydroxycyclohexyl phenyl ketone, poly(ethylene glycol) methacrylate with an average molecular weight of 500 and poly(ethylene glycol) methyl ether methacrylate with an average molecular weight of 2000) were acquired from Sigma-Aldrich. Borosilicate glass microscope slides (1 mm thickness) were supplied by Carl Roth. Dow Corning and BAP Medical B.V. provided PDMS Sylgard 184 silicone elastomer kit and biopsy punches of 1 mm diameter, respectively.

Polyethyleneglycol methyl ether methacrylate (PEGMA, M_n = 300 g/mol, average degree of polymerization ≈5) (Sigma-Aldrich, Darmstadt, Germany), triethyleneglycol methyl ether methacrylate (TEGMA M_n = 232 g/mol) (Sigma-Aldrich, Darmstadt, Germany), and perfluorohexylethyl acrylate (FA, Fluorochem, Hadfield, United Kingdom) were passed through basic alumina to remove inhibitors. N,N,N′,N″,N″-pentamethyldiethylenetriamine (PMDETA, Sigma-Aldrich) and ethyl α-bromophenylacetate (EBPA, Sigma-Aldrich, Darmstadt, Germany) were distilled under vacuum. CuBr₂ (Sigma-Aldrich, Darmstadt, Germany) was recrystallized from water solution. Toluene (Sigma-Aldrich, Darmstadt, Germany) was distilled at atmospheric pressure after reflux over calcium hydride. 3-Hydroxyanisaldehyde, 3,4,5-trimethoxyphenylacetic acid, acetic anhydride, triethyl amine, and copper (powder) were used as received (Sigma-Aldrich, Darmstadt, Germany). Quinoline was distilled under reduced pressure before use.
The preparation of the cis-Combretastatin A-4 (CA-4) was carried out following a two-step literature procedure, obtaining the desired product with a 98% GLC purity and a 40% yield [61 (link)].
Common laboratory solvents and other reagents (Sigma-Aldrich, Darmstadt, Germany) were used as received.

Histones from calf thymus, bovine serum albumin, N-isopropylacrylamide (NIPAm), sodium dodecyl sulfate (SDS), fluoresceinamine isomer, and poly(ethylene glycol) methyl ether methacrylate (average M_n 500, 1500, and 4000) were purchased from Sigma Aldrich. N,N’-methylenebisacrylamide (BIS) was from Fluka; N-t-butylacrylamide (TBAm) was from ACROS ORGANICS. [2,3-14C]-Acrylamide was purchased from GE Healthcare UK Ltd. (Buckinghamshire, UK). Histone H1, H2A, H2B, H3.3 and H4 (Human, Recombinant) were purchased from New England Biolabs, Inc., (MA, USA). Sulfo-Cy5 NHS ester was purchased from Lumiprobe (FL, USA). 5-(and 6)-carboxytetramethylrhodamine, succinimidyl ester (NHS-Rhodamine) was purchased from Thermo Fisher Scientific, Inc.

Iron (III) chloride hexahydrate ACS, sodium acetate (anhydrous ACS), ethylene glycol, ammonium hydroxide 28–30%, ammonium persulfate (APS) (≥98%, Pro-Pure, Proteomics Grade), ethanol (reagent alcohol ACS), and methanol (≥99.8% ACS) were purchased from VWR. N,N′-Methylenebisacrylamide (99%) was purchased from EMD Millipore. Trisodium citrate dihydrate (ACS reagent, ≥99.0%), tetraethyl orthosilicate (TEOS) (reagent grade, 98%), 3-(trimethoxysilyl)propyl methacrylate (MPS) (98%), and poly(ethylene glycol) methyl ether methacrylate (OEGMA, average Mn 500, contains 100 ppm MEHQ as inhibitor, 200 ppm BHT as inhibitor) were purchased from Sigma–Aldrich. 4,4′-Azobis(4-cyanovaleric acid) (ACVA, 98%, cont. ca 18% water) and divinylbenzene (DVB, 80%, mixture of isomers) were purchased from Alfa Aesar and purified by passing a short silica column to remove the inhibitor. N-(3-Dimethylaminopropyl)methacrylamide (DMAPMA) was purchased from TCI and also purified by passing a short silica column to remove the inhibitor. The ELISA kit to measure human C-reactive protein (CRP) was purchased from R&D Systems (Minneapolis, MN). Human CRP protein purified from human serum was from Sigma–Aldrich.

Glyceryl monomethacrylate (GMA; 98%, supplied
by GEO Specialty Chemicals), poly(ethylene glycol) methyl ether acrylate
(PEGA, M_n = 480, Sigma-Aldrich), poly(ethylene
glycol) methyl ether methacrylate (PEGMA, M_n = 950, Sigma-Aldrich), acrylic acid (AA, 99%; Fisher), methacrylic
acid (MAA, ≥99%; Merck), lauryl acrylate (LA, >98%, TCI
Chemicals), iso-octyl acrylate (ⁱOA, >90%;
Sigma-Aldrich),
2-ethylhexyl acrylate (EHA, Alfa Aesar, 98%), iso-bornyl acrylate (ⁱBA 99%; Alfa Aesar), tert-butyl acrylate (^tBuA, 99%; Alfa Aesar), 3,5,5-trimethylhexyl
acrylate (TMHA; Sigma-Aldrich), 3,5,5-trimethylhexyl methacrylate
(TMHMA, ABCR), and tert-octyl acrylamide (OAA; supplied
by ABCR, Germany) were all used as received. Asoisobutyronitrile (AIBN)
was recrystallized twice from mEthanol. Ethanol (99.8%, Fisher), mEthanol
(>99%, Sigma-Aldrich), THF, and n-heptane (>99%,
Sigma-Aldrich) were used as received. 2-(Dodecylthiocarbonothioylthio)-2-methylpropionic
acid (DDMAT, 98%) was purchased from Sigma-Aldrich and used as received.
MilliQ water was obtained from an Elga Elgastat Option 3A Water Purifier
system.