Human il 37 elisa kit
The Human IL-37 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-37 (IL-37) levels in various biological samples.
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4 protocols using human il 37 elisa kit
Quantifying Secreted IL-37 and VEGF
Quantification of CXCL8 and IL-37 Secretion and Intracellular Levels
To measure the intracellular production of IL-37, protein extraction from cells was performed in radio-immunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, St Louis, MO, USA) containing a 1:100 protease inhibitor cocktail (catalogue no: P8340, Sigma-Aldrich) [6 (link)]. The detection of intracellular IL-37 from cell lysate was performed using a human IL-37 ELISA Kit (Adipogen, Life Sciences, Liestal, Switzerland) due to higher sensitivity (10 pg/ml). Protein levels were calculated using a recombinant IL-37 protein standard curve according to the manufacturer’s instructions.
Quantification of IL-37 in Plasma Samples
Quantifying IL-37 Levels in Samples
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