2 n morpholino ethane sulfonic acid mes

Phosphate-buffered saline (PBS) (137 mM NaCl, 8.1 mM Na₂HPO₄, 2.68 mM KCl, 1.47 mM KH₂PO₄, pH 7.4), toluene, acetone, dimethyl sulfoxide (DMSO), 1-adamantanamine (Amino-Ad), and D₂O were purchased from Wako Pure Chemical Industries (Osaka, Japan). Ethanol was purchased from Shinwa Alcohol Industry (Tokyo, Japan). Mono-(6-amino-6-deoxy)-β-cyclodextrin (Amino–βCD) was prepared following a reported procedure [26 (link)]. Mono-6-(deoxy-acrylamido)-β-cyclodextrin (βCD–AAm) and adamantane–acrylamide (Ad–AAm) were obtained from Yushiro Chemical Industry (Tokyo, Japan). Sodium hydroxide, acrylamide (AAm), 3-aminopropyltriethoxysilane (APTES), N-hydroxysuccinimide (NHS), lithium bromide (LiBr), and 2-(N-morpholino)ethane sulfonic acid (MES) were purchased from Nacalai Tesque (Kyoto, Japan). Lithium phenyl (2,4,6-trimethylbenzoyl) phosphinate (LAP), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and 1-adamantanecarboxylic acid were purchased from Tokyo Chemical Industry (Tokyo, Japan). Gelatin type A from porcine skin (bloom strength of ~300), gelatin type B from bovine skin (bloom strength of ~225), and methacrylic anhydride were purchased from Sigma–Aldrich (Tokyo, Japan). Water used to prepare aqueous solutions was purified using a Millipore Integral MT system (Tokyo, Japan). Unless otherwise stated, these reagents were used without further purification.

COATSOME^® SS-OP was supplied by NOF CORPORATION (Tokyo, Japan), and 1,2-Dimyristoyl-rac-glycero-3-methylpolyoxyethylene-2000 (DMG-PEG2000), DOPC was purchased from NOF CORPORATION. Dilinoleoyl-PS was purchased from Avanti Polar Lipids. Cholesterol was purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS) without Ca²⁺ and Mg²⁺, tert-Butanol, DL-malic acid, 2-(N-morpholino) ethanesulfonic acid (MES), RPMI-1640 medium with L-Glutamine and bovine serum albumin (BSA) were purchased from Nacalai Tesque (Kyoto, Japan). Amicon Ultra-15–100 K centrifugal units were purchased from Merck Millipore (Burlington, MA, USA). Antibodies are listed in Table S2.