Phycoerythrin pe conjugated antibody

Manufactured by Miltenyi Biotec

Sourced in Germany

Phycoerythrin (PE)-conjugated antibody is a fluorescent labeling reagent used in flow cytometry applications. PE is a bright, red-orange fluorescent protein derived from red algae. When conjugated to an antibody, it enables the detection and identification of specific cell surface or intracellular targets.

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Lab products found in correlation

Anti human cd71 fluorescein isothiocyanate fitc conjugated antibody, by Miltenyi Biotec (2 mentions) Anti human cd235a, by Miltenyi Biotec (2 mentions) Facscanto 2 system, by BD (1 mentions)

Spelling variants of this product

Phycoerythrin (PE; (4 citations) R‐phycoerythrin (PE)‐conjugated anti‐CD44 monoclonal antibody (2 citations)

2 protocols using phycoerythrin pe conjugated antibody

Erythroid Differentiation Status of K562 Cells

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The erythroid differentiation status of K562 cells and K562(BCL11A-XL) clone 12 was investigated by studying transferrin Receptor (trfR) and glycophorin A (GYPA) expression by fluorescence-activated cell sorting (FACS) analysis using anti-human CD71 fluorescein isothiocyanate (FITC)-conjugated antibody (Miltenyi Biotec GmbH, Germany) and anti-human CD235a (glycophorin A) phycoerythrin (PE)-conjugated antibody (Miltenyi Biotec) [36] .

Finotti A., Gasparello J., Breveglieri G., Cosenza L.C., Montagner G., Bresciani A., Altamura S., Bianchi N., Martini E., Gallerani E., Borgatti M, & Gambari R. (2015). Development and characterization of K562 cell clones expressing BCL11A-XL: Decreased hemoglobin production with fetal hemoglobin inducers and its rescue with mithramycin. Experimental Hematology, 43(12), 1062-1071.e3.

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Erythroid Differentiation by FACS Analysis

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The erythroid differentiation status of ErPCs was investigated by studying transferrin Receptor (trfR) and glycophorin A (GPA) expression by fluorescence-activated cell sorting (FACS) analysis using anti-human CD71 fluorescein isothiocyanate (FITC)-conjugated antibody (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and anti-human CD235a (glycophorin A) phycoerythrin (PE)-conjugated antibody (Miltenyi Biotec). According to the manufacturer’s protocol, 10 μL of antibodies were added to freshly isolated cells in 100 μL 1× PBS and 1% FBS, and cells were incubated on ice for 30 min. Cells were washed twice in 1× PBS and 1% FBS and analyzed using the BD FACS Canto II system (Becton-Dickinson, Franklin Lakes, NJ, USA). Data were analyzed using FACSDiva 8.0 software (Becton-Dickinson, Franklin Lakes, NJ, USA).

Gasparello J., Fabbri E., Bianchi N., Breveglieri G., Zuccato C., Borgatti M., Gambari R, & Finotti A. (2017). BCL11A mRNA Targeting by miR-210: A Possible Network Regulating γ-Globin Gene Expression. International Journal of Molecular Sciences, 18(12), 2530.

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