After intragastric administration of La2O3 NPs and MPs, the levels of MDA and activities of SOD and CAT in the testis were tested using the following assay kits: A001-1-2, A003-3-1 and A007-2-1 (Nanjing Jiancheng Bioengineering Institute, China). The steps were based on the manufacturers’ protocol. All assays were performed in triplicate.
A003 3 1
Manufactured by Nanjing Jiancheng
Sourced in China
The A003-3-1 is a laboratory equipment product from Nanjing Jiancheng. It is a piece of scientific apparatus designed for specific tasks within a laboratory environment. The core function of this product is to perform measurements or analyses, but without further details on its intended use or capabilities.
Automatically generated - may contain errors
Lab products found in correlation
A084 3 1, by Nanjing Jiancheng (3 mentions)
A001 1 2, by Nanjing Jiancheng (2 mentions)
A007 1 1, by Nanjing Jiancheng (2 mentions)
A064 1 1, by Nanjing Jiancheng (2 mentions)
A007 2 1, by Nanjing Jiancheng (1 mentions)
Synergy htx, by Agilent Technologies (1 mentions)
A137 1 1, by Nanjing Jiancheng (1 mentions)
A107 1 1, by Nanjing Jiancheng (1 mentions)
5 protocols using a003 3 1
1
Intragastric La2O3 NPs and MPs Impact on Testis Oxidative Stress
2
Maize Leaf Antioxidant Enzyme Analysis
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Fresh leaves (the ear leaf) of maize were taken at the tasseling stage for the determination of leaf antioxidant enzyme activities and malondialdehyde (MDA) content. Specifically, 0.2 g of fresh leaves were placed in a precooled mortar, and 1.8 mL phosphate buffer (pH = 7.8) was added to the ice bath to grind the leaves into a homogenate. The grinding solution was transferred into a 2 mL centrifuge tube and centrifuged at 12000 r·min-1 for 10 min at 4°C. The supernatant, which was the crude enzyme extract, was stored at -20°C for further use for the determination of peroxidase (POD) and superoxide dismutase (SOD) activities. Alternatively, the phosphate buffer used above was replaced with saline extract to obtain the enzyme extract for the determination of catalase (CAT) activity. The extract was replaced with the extract from the MDA kit, and the obtained enzyme extract was used for the determination of MDA content.
Then, the enzyme extracts were further processed for POD, SOD, CAT and MDA measurements using A084-3-1, T001-1, A007-1-1 and A003-3-1 kits (Nanjing Jiancheng Bioengineering Institute), respectively. Finally, the absorbance values were measured at 420 nm, 550 nm, 405 nm and 532 nm using a plate reader (Synergy HTX, BioTek, USA).
Then, the enzyme extracts were further processed for POD, SOD, CAT and MDA measurements using A084-3-1, T001-1, A007-1-1 and A003-3-1 kits (Nanjing Jiancheng Bioengineering Institute), respectively. Finally, the absorbance values were measured at 420 nm, 550 nm, 405 nm and 532 nm using a plate reader (Synergy HTX, BioTek, USA).
3
Physiological Response Assays for Salt-Stressed Plants
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The physiological indicators: PAL, H2O2, MDA, Pro, and POD were measured using detection assay kits (Cat. No. A137-1-1, A064-1-1, A003-3-1, A107-1-1 and A084-3-1 respectively; Nanjing Jiancheng Bioengineering Institute, China). All the tests were performed according to the manufacturer’s instructions (http://www.njjcbio.com/ , accessed on 1 December 2022). A lignin test kit was obtained from COMIN company (Cat. No. MZS-1-G; Suzhou, China). The leaves were used for physiological detection. Expanded leaves were collected from L96 and NT plants after salt stress (0 d, 2 d and 5 d). Each sample was repeated 4 times. At the same time, root were collected, washed, and frozen in −80 °C for the following RNA extraction and plant hormone detection via HPLC (Agilent 1260, Santa Clara, CA, USA).
4
Evaluating Fat and MDA in Brown Rice
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The fat content in 1 g brown rice flour was determined using the Soxhlet extraction method with petroleum ether. One gram of brown rice flour was extracted with ethanol (1:2), phenolphthalein solution was then added, and the solution was titrated with 0.1 M KOH to determine the total free fat acid contents. The MDA content of brown rice was determined using an MDA assay kit (A003-3–1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions.
5
Antioxidative Biomarker Determination in Plant Tissues
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A certain weight of leaf or shoot sample was crushed in a prechilled pestle and crushed with a mortar in an appropriate volume of prechilled extraction buffer. After uniform homogenization and centrifugation at 4000 g for 15 min at 4 °C, the supernatant was collected and used for biochemical analyses.
Antioxidative capabilities were estimated by quantifying peroxidation products and antioxidase activity levels. The malondialdehyde (MDA) content was determined using a Plant MDA Assay Kit (TBA method; A003-3-1, Nanjing Jiancheng, Nanjing, China). The hydrogen peroxide (H 2 O 2 ) level was determined using an H 2 O 2 Assay Kit (H 2 O 2 reacted with titanium salt and is then quantified at OD 415 ; A064-1-1, Nanjing Jiancheng), and the superoxide anion (O 2 -) level was determined using an O 2 -Assay Kit (sulfanilamide method; R30342, Shanghai Yuanye, Shanghai, China). The activity levels of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were determined using the Total SOD (WST-1 method; A001-1-2, Nanjing Jiancheng), POD (H 2 O 2 catalysis method; A084-3-1, Nanjing Jiancheng) and CAT (H 2 O 2 catalysis method; A007-1-1, Nanjing Jiancheng) Assay Kits, respectively, following the manufacturer's instructions. All the leaf and shoot samples were measured three times in parallel.
Antioxidative capabilities were estimated by quantifying peroxidation products and antioxidase activity levels. The malondialdehyde (MDA) content was determined using a Plant MDA Assay Kit (TBA method; A003-3-1, Nanjing Jiancheng, Nanjing, China). The hydrogen peroxide (H 2 O 2 ) level was determined using an H 2 O 2 Assay Kit (H 2 O 2 reacted with titanium salt and is then quantified at OD 415 ; A064-1-1, Nanjing Jiancheng), and the superoxide anion (O 2 -) level was determined using an O 2 -Assay Kit (sulfanilamide method; R30342, Shanghai Yuanye, Shanghai, China). The activity levels of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were determined using the Total SOD (WST-1 method; A001-1-2, Nanjing Jiancheng), POD (H 2 O 2 catalysis method; A084-3-1, Nanjing Jiancheng) and CAT (H 2 O 2 catalysis method; A007-1-1, Nanjing Jiancheng) Assay Kits, respectively, following the manufacturer's instructions. All the leaf and shoot samples were measured three times in parallel.
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