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Anti cxcr3 alexafluor 488 clone 1c6 cxcr3

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Anti-CXCR3-AlexaFluor 488 (clone 1C6/CXCR3) is a fluorescently-labeled monoclonal antibody that binds to the CXCR3 chemokine receptor. CXCR3 is a G protein-coupled receptor that plays a role in the trafficking and activation of T cells. The AlexaFluor 488 dye allows for flow cytometric detection and analysis of CXCR3-expressing cells.

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Lab products found in correlation

Aqua live dead amine dye amcyan, by Thermo Fisher Scientific (2 mentions) Anti cd8 qdot705 clone 3b5, by Thermo Fisher Scientific (2 mentions) Anti cd161 pe clone hp 3g10 and anti il 17 alexa488 clone ebio64dec17, by Thermo Fisher Scientific (2 mentions) Anti cd28 ecd clone cd28.2, by Beckman Coulter (2 mentions) Anti cd161 bv421 clone hp 3g10, by BioLegend (2 mentions)

Close spelling variants of this product

Anti-CXCR3

2 protocols using anti cxcr3 alexafluor 488 clone 1c6 cxcr3

1

Comprehensive Immune Profiling by Flow Cytometry

Cited 1 time
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Fourteen-parameter flow cytometric analysis was performed on peripheral blood, LN, and RB derived cells according to standard procedures using a panel of monoclonal antibodies that we and others have shown to be cross-reactive with SMs and RMs.12 (link) The following antibodies were used at predetermined optimal concentrations: anti-CCR6-PE-Cy7 (clone 11A9), anti-CCR5-PE and -APC (clone 3A9), anti-CD3-APC-Cy7 (clone SP34-2), anti-CD62L-FITC (clone SK11), anti-CD95-PE-Cy5 (clone DX2), anti-Ki-67-Alexa700 (clone B56), anti-IFN-γ-Alexa700 (clone B27), and anti-CXCR3-AlexaFluor 488 (clone 1C6/CXCR3) from BD Biosciences; anti-CD161-PE (clone HP-3G10) and anti-IL-17-Alexa488 (clone eBio64DEC17) from eBioscience; anti-CD28-ECD (clone CD28.2) from Beckman Coulter; anti-CD4-BV421 (clone OKT4), anti-CD4-BV605 (clone OKT4), and anti-CD161-BV421 (clone HP-3G10) from Biolegend; anti-CD8-Qdot705 (clone 3B5) and Aqua Live/Dead amine dye-AmCyan from Invitrogen. Flow cytometric acquisition was performed on at least 100,000 CD3+ T cells on an LSRII cytometer driven by the FACS DiVa software, or at least 10,000 CD3+ T cells for rectal biopsy-derived cells. The data acquired were analyzed using FlowJo software (version 9.8.5; TreeStar).
McGary C.S., Alvarez X., Harrington S., Cervasi B., Ryan E.S., Iriele R.I., Paganini S., Harper J., Easley K., Silvestri G., Ansari A.A., Lichterfeld M., Micci L, & Paiardini M. (2017). The loss of CCR6+ and CD161+ CD4+ T cell homeostasis contributes to disease progression in SIV-infected rhesus macaques. Mucosal immunology, 10(4), 1082-1096.
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2

Comprehensive Immune Profiling by Flow Cytometry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fourteen-parameter flow cytometric analysis was performed on peripheral blood, LN, and RB derived cells according to standard procedures using a panel of monoclonal antibodies that we and others have shown to be cross-reactive with SMs and RMs.12 (link) The following antibodies were used at predetermined optimal concentrations: anti-CCR6-PE-Cy7 (clone 11A9), anti-CCR5-PE and -APC (clone 3A9), anti-CD3-APC-Cy7 (clone SP34-2), anti-CD62L-FITC (clone SK11), anti-CD95-PE-Cy5 (clone DX2), anti-Ki-67-Alexa700 (clone B56), anti-IFN-γ-Alexa700 (clone B27), and anti-CXCR3-AlexaFluor 488 (clone 1C6/CXCR3) from BD Biosciences; anti-CD161-PE (clone HP-3G10) and anti-IL-17-Alexa488 (clone eBio64DEC17) from eBioscience; anti-CD28-ECD (clone CD28.2) from Beckman Coulter; anti-CD4-BV421 (clone OKT4), anti-CD4-BV605 (clone OKT4), and anti-CD161-BV421 (clone HP-3G10) from Biolegend; anti-CD8-Qdot705 (clone 3B5) and Aqua Live/Dead amine dye-AmCyan from Invitrogen. Flow cytometric acquisition was performed on at least 100,000 CD3+ T cells on an LSRII cytometer driven by the FACS DiVa software, or at least 10,000 CD3+ T cells for rectal biopsy-derived cells. The data acquired were analyzed using FlowJo software (version 9.8.5; TreeStar).
McGary C.S., Alvarez X., Harrington S., Cervasi B., Ryan E.S., Iriele R.I., Paganini S., Harper J., Easley K., Silvestri G., Ansari A.A., Lichterfeld M., Micci L, & Paiardini M. (2017). The loss of CCR6+ and CD161+ CD4+ T cell homeostasis contributes to disease progression in SIV-infected rhesus macaques. Mucosal immunology, 10(4), 1082-1096.
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