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Rat insulin elisa kit

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The Rat Insulin ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of insulin levels in rat samples. The kit uses a specific antibody coated on the plate to capture insulin from the sample, and a second antibody conjugated to a detection enzyme to quantify the amount of insulin present.

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Lab products found in correlation

Test reagent kits, by Biodiagnostic (1 mentions) Glucose lq kit, by Spinreact (1 mentions) 737 automatic analyzer, by Hitachi (1 mentions)

Close spelling variants of this product

Rat ELISA Kit (2 citations) Rat Insulin High Sensitivity ELISA kit (2 citations)

3 protocols using rat insulin elisa kit

1

Metabolic Biomarkers Evaluation Protocol

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Fasting serum glucose (mg/dl) was determined colorimetrically using a test reagent kit (Biolabo SA, France)

Determination of serum insulin (μIU/ml) was performed using rat insulin ELISA kit (Biovendor, Czech Republic)

HOMA-IR score was used as an index of IR as described by Matthews et al.[7 (link)] using the following formula:

HOMA-IR score = serum glucose (mmol/l) × serum insulin (μIU/ml)/22.5

TNF-α level (pg/ml) was determined using a test reagent kit (ID labs, Canada)

Serum total cholesterol and TG were estimated using test reagent kits (Spinreact, Spain) and expressed as mg/dl

Uric acid, urea and creatinine levels were determined using test reagent kits (Biodiagnostics, Egypt) and expressed as mg/dl

Blood GSH, serum MDA, and liver nitric oxide (total nitrates and nitrites, NOx) were determined according to the methods described previously.[8 (link)9 (link)10 (link)]

, & Abo-youssef A.M. (2015). Protective effect of rosiglitazone, quercetin, and their combination on fructose-induced metabolic syndrome in rats. Indian Journal of Pharmacology, 47(6), 620-626.
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2

Metabolic Biomarker Quantification Protocol

Cited 1 time
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Serum fasting glucose assayed by glucose oxidase technique using (Glucose-LQ kit, Spinreact) (El-Gayar et al., 2012 ). Insulin in serum was assayed via rat insulin ELISA kit (Biovendor Laboratory Medicine, Brno, Czech Republic), according to the manufacturer’s procedures. Homeostatic model assessment of insulin resistance (HOMA-IR) is calculated via this equation: HOMA-IR = fasting blood glucose (mg/dl) × fasting insulin (ng/ml)/405 (Roza et al. , 2016 (link)). Total cholesterol (TC) was assessed by the CHOD-PAP-enzymatic colorimetric method (Penttilä et al., 1981), and triglycerides (TG) by the GPO-PAP-enzymatic colorimetric method (Bucolo and David, 1973 (link)). Serum HDL-c and LDL-c were assessed by direct enzymatic colorimetric liquid method (Friedman and Young, 1989 ), and the lipid parameters were measured using commercially accessible kits (Spinreact, Spain). Serum testosterone was assessed by Rat Testosterone ELISA, Kit Cat.No.SE120089 (Sigma Aldrich, St Louis, MO, USA). Serum FSH and LH were assessed by Rat FSH ELISA Kit Cat. No. CSB-E06869r, and Rat LH ELISA Kit Cat.No.CSB-E12654r respectively, from CUSABIO.
Refaat H., Dowidar M.F., Ahmed A.I., Khamis T., Abdelhaleem S.E, & Abdo S.A. (2024). The corrective role of superparamagnetic iron oxide nanoparticles for the genes controlling hypothalamus-pituitary-testis-axis in male obesity-associated secondary hypogonadism. Open Veterinary Journal, 14(1), 428-437.
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3

Plasma Metabolite Profiling in Preclinical Studies

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The following plasma metabolites were measured: basal glucose, triglycerides, total cholesterol, high-density lipoprotein (HDL), urea, bilirubin, alkaline phosphatase (ALKP), and the hepatic enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltranspeptidase (γGT). These metabolites were analyzed using commercial kits according to the manufacturer’s instructions and a Hitachi 737 Automatic Analyzer (Hitachi Ltd, Tokyo, Japan). Very low-density lipoprotein (VLDL) was estimated following the Friedewald equations [46 (link)] and low-density lipoprotein (LDL) was determined by the modification of Friedewal equation proposed by Ahmadi et al. [47 (link)]: (VLDL = TG/5); LDL = [(TChol/1.19)+(TG/1.9)- (HDL/1.1)-38]. The leptin levels in plasma were measured using a commercial rat insulin ELISA kit (Cat. no. RD291001200R; BioVendor, Brno, Czech Republic).
Ramírez-López M.T., Arco R., Decara J., Vázquez M., Noemí Blanco R., Alén F., Suárez J., Gómez de Heras R, & Rodríguez de Fonseca F. (2016). Exposure to a Highly Caloric Palatable Diet during the Perinatal Period Affects the Expression of the Endogenous Cannabinoid System in the Brain, Liver and Adipose Tissue of Adult Rat Offspring. PLoS ONE, 11(11), e0165432.
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