Ps824 kap1

Manufactured by Fortis Life Sciences

The PS824-KAP1 is a laboratory equipment product. It serves as a core function, but a detailed and unbiased description cannot be provided while maintaining conciseness.

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Lab products found in correlation

Protease inhibitor, by Merck Group (5 mentions) β actin, by Merck Group (4 mentions) Phosphatase inhibitor, by Merck Group (3 mentions) Ps15 p53, by Cell Signaling Technology (3 mentions) Atm 2c1, by Santa Cruz Biotechnology (3 mentions) Hrp conjugated goat anti mouse rabbit igg, by Merck Group (3 mentions) Ab4731, by Abcam (2 mentions) C myc, by Merck Group (2 mentions) P t1989 atr, by GeneTex (2 mentions) Chk2 pt68, by Cell Signaling Technology (2 mentions) Gapdh hrp, by Abcam (2 mentions) α tubulin, by Abcam (2 mentions) Ps1981 atm, by Cell Signaling Technology (2 mentions) P chk1 s317, by Cell Signaling Technology (2 mentions) P s966 smc1, by Fortis Life Sciences (2 mentions) Ps33 rpa, by Fortis Life Sciences (2 mentions) Ub pcna lys 164, by Cell Signaling Technology (2 mentions) Pcna hrp, by Santa Cruz Biotechnology (2 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (2 mentions) Flag m2, by Merck Group (2 mentions)

5 protocols using ps824 kap1

Immunoblotting Assay for Protein Analysis

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Cells were extracted in cell lysis buffer (NEB) supplemented with protease inhibitors (Sigma) and phosphatase inhibitors (Sigma, NEB). Protein samples were separated by SDS–PAGE, and subsequently transferred onto PVDF membranes. Immunoblots were performed using standard procedures. All primary antibodies were used at 1:1,000 dilution and secondary antibodies at 1:10,000, except β-Actin-HRP antibody, used at 1:50,000. The following antibodies were used: ATM (Santa Cruz, sc23931), b-ACTIN-HRP (Abcam, ab49900), p53 (Cell Signaling, 2524S), pS824-KAP1 (Bethyl Labs, A300-767A), KAP1 (Abcam, ab10484), HRP-conjugated anti-Mouse (Jackson, 115-035-174), HRP-conjugated anti-Rabbit (Jackson, 115-032-171).

Foster H., Ruiz E.J., Moore C., Stamp G.W., Nye E.L., Li N., Pan Y., He Y., Downward J, & Behrens A. (2019). ATMIN is a tumor suppressor gene in lung adenocarcinoma. Cancer research, 79(20), 5159-5166.

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Western Blot Analysis of DNA Damage Response

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Cells were extracted in 20mM Tris, pH7.5, 150mM NaCl, 0.1% NP-40, 10% glycerol (lysis buffer) supplemented with protease inhibitors (Sigma). Protein samples were separated by SDS–PAGE, and subsequently transferred onto polyvinylidene difluoride membranes. All primary antibodies were used at 1:1000 dilution and secondary antibodies at 1:2000 unless otherwise indicated. The following antibodies were used: ATMIN (AB3271, Millipore, used at 1:5000), WRNIP1 (ab4731, AbCam, used at 1:10,000), RAD18 (S2980, Epitomics) pS1981-ATM (10H11.E12, Cell Signalling Technology), ATM (2C1, Santa Cruz), p-T1989-ATR (GTX128145, GeneTex), ATR (sc28901, Santa Cruz), pS824-Kap1 (Bethyl Laboratories), Kap1 (Bethyl Laboratories), p-S317-Chk1 (2344, Cell Signalling Technology), Chk1 (sc8408, Santa Cruz), p-S966-Smc1 (A300-050A, Bethyl Laboratories), Smc1 (ab9262, AbCam), p53 (DO-1, Santa Cruz), pS15-p53 (Cell Signalling Technology), pT68-Chk2 (Cell Signalling Technology), Chk2 (Clone 7, Millipore), pS33-RPA (A300-246A, Bethyl Laboratories), RPA (sc56770, Santa Cruz), Ub-PCNA (Lys 164) (13439, Cell Signaling Technology), PCNA (2586, Cell Signaling Technology), PCNA-HRP (Clone PC10, sc56 Santa Cruz), His (A00186-100, Genscript), Flag M2 (Sigma), c-Myc (Sigma), β-actin (Sigma), alpha-tubulin (ab7291, AbCam), GAPDH-HRP (ab9385, Abcam) and HRP-conjugated goat anti-mouse/rabbit IgG (Sigma).

Kanu N., Zhang T., Burrell R.A., Chakraborty A., Cronshaw J., Da Costa C., Grönroos E., Pemberton H.N., Anderton E., Gonzalez L., Sabbioneda S., Ulrich H.D., Swanton C, & Behrens A. (2015). RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene, 35(30), 4009-4019.

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Immunoblotting of DNA Damage Response

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Cells were extracted in RIPA lysis buffer (NEB) supplemented with protease inhibitors (Sigma) and phosphatase inhibitors (Sigma, NEB). Immunoblots were performed using standard procedures. Protein samples were separated by SDS–PAGE (3–8% gradient gels; Invitrogen), and subsequently transferred onto nitrocellulose membranes. All primary antibodies were used at 1:1000 dilution, except for P-S957-SMC1 that was used at 1:400, and secondary antibodies at 1:5000. The following antibodies were used: ATM 2C1 (Santa Cruz), P-S1981-ATM (10H11.E12; NEB), ASCIZ (Millipore), P-S824-KAP1 (Bethyl Laboratories, Inc), KAP1 (Bethyl Laboratories, Inc), P-S15-p53 (16G8; NEB), P-S957-SMC1 (5D11G5; Millipore), SMC1 (Abcam), P-S317-CHK1 (NEB), CHK1 (DCS-310; Santa Cruz), FANCD2 (EPR2302; Abcam), p95/NBS (NEB), β-actin (Sigma), 53BP1 (H300; Santa Cruz), CHK1 (DCS-310; Santa Cruz), HRP-conjugated goat anti-mouse/rabbit IgG (Sigma).

Schmidt L., Wiedner M., Velimezi G., Prochazkova J., Owusu M., Bauer S, & Loizou J.I. (2014). ATMIN is required for the ATM-mediated signaling and recruitment of 53BP1 to DNA damage sites upon replication stress. DNA Repair, 24, 122-130.

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Western Blot Analysis of DNA Damage Response

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Cells were extracted in RIPA lysis buffer (NEB) supplemented with protease inhibitors (Sigma) and phosphatase inhibitors (Sigma, NEB). Western blots were performed using standard procedures. Protein samples were separated by SDS–PAGE (3–8% or 4–12% gradient gels; Invitrogen), and subsequently transferred onto nitrocellulose membranes. All primary antibodies were used at 1:1000 dilution and secondary antibodies at 1:5000. The following antibodies were used: ATM (Santa Cruz), ASCIZ (Millipore); p95 (known as NBS1) (NEB), β-actin (Sigma), pS15-p53 (Cell Signalling), pS824-KAP1 (Bethyl Labs), total p53 (Pab-421; CR-UK generated antibody) and HRP-conjugated goat anti-mouse or rabbit IgG (Sigma).

Prochazkova J., Sakaguchi S., Owusu M., Mazouzi A., Wiedner M., Velimezi G., Moder M., Turchinovich G., Hladik A., Gurnhofer E., Hayday A., Behrens A., Knapp S., Kenner L., Ellmeier W, & Loizou J.I. (2015). DNA Repair Cofactors ATMIN and NBS1 Are Required to Suppress T Cell Activation. PLoS Genetics, 11(11), e1005645.

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Western Blot Analysis of DNA Damage Response

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