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Csb e04650m

Manufactured by Cusabio
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Sourced in China, United States

The CSB-E04650m is a laboratory equipment product. It serves a core function in the laboratory environment.

Automatically generated - may contain errors

Lab products found in correlation

Multiskan skyhigh, by Thermo Fisher Scientific (1 mentions) Csb e07272m, by Cusabio (1 mentions) Am202 k, by Asan Pharmaceutical (1 mentions)

2 protocols using csb e04650m

1

Serum Leptin Measurement Protocol

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Blood samples were collected (between 12 and 2PM) via cardiac puncture and centrifuged at 845×g for 10 min at room temperature and extracted serum stored at −80 °C. Commercial ELISA kits were used for the measurement of leptin (CSB-E04650m, Cusabio, China) as per manufacturer's instructions.
Tangseefa P., Jin H., Zhang H., Xie M, & Ibáñez C.F. (2024). Human ACVR1C missense variants that correlate with altered body fat distribution produce metabolic alterations of graded severity in knock-in mutant mice. Molecular Metabolism, 81, 101890.
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2

Biochemical Analysis of Blood Samples

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All the biochemical tests were performed using commercial kits. Whole blood was collected using EDTA vacutainers. Serum was separated by centrifuging the blood at 3000× g 10 min at 4 °C. Triglyceride (TG, AM1575K) and total cholesterol (TC, AM202K) were detected using commercial test kits from Asan Pharmaceutical, Seoul, Republic of Korea. Plasma levels of adiponectin (CSB-E07272m, CUSABIO, Houston, TX, USA) and leptin (CSB-E04650m, CUSABIO, Houston, TX, USA) were determined using commercial sandwich ELISA kits. The absorbance at 450 nm was detected using Multiskan SkyHigh microplate spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The unknown sample concentration (ng/mL) was determined from the standard curve.
Lee H.Y., Lee G.H., Yoon Y., Hoang T.H, & Chae H.J. (2022). IBF-R Regulates IRE1α Post-Translational Modifications and ER Stress in High-Fat Diet-Induced Obese Mice. Nutrients, 14(1), 217.
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