Peritoneal cells were blocked for 10 min with 5 µg/ml anti-CD16/CD32 antibody (clone 2.4G2, BD Biosciences) and stained for 30 min on ice with the following antibodies (1 µl each per 106 cells): rat anti-F4/80-FITC (clone BM8, eBioscience), rat anti-CD45-V500 (clone 30-F11, BD Biosciences), rat anti-CD11b-V450 (clone M1/70, BD Biosciences), rat anti-Ly6G-PerCP-Cy5.5 (clone 1A8, BD Biosciences), rat anti-CD3-PE (clone 17A2, BD Biosciences), rat-anti-CD19-PE-Cy7 (clone 1D3, BD Biosciences). Cells were analyzed with a BD FACSCanto II flow cytometer and FlowJo software (Tree Star).
Rat anti cd45 v500 clone 30 f11
Manufactured by BD
The Rat anti-CD45-V500 (clone 30-F11) is a laboratory reagent used for flow cytometry applications. It is an antibody that specifically binds to the CD45 protein, which is expressed on the surface of various hematopoietic cells. The V500 fluorochrome is conjugated to the antibody, allowing for detection and analysis of CD45-positive cells.
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Lab products found in correlation
Anti cd16 cd32 antibody clone 2.4g2, by BD (1 mentions)
Cd3 apc, by BioLegend (1 mentions)
Rat anti cd11b pecy7, by Thermo Fisher Scientific (1 mentions)
Onecomp ebeads, by Thermo Fisher Scientific (1 mentions)
Cd4 apc efluor 780, by Thermo Fisher Scientific (1 mentions)
Cd80 fitc, by BioLegend (1 mentions)
Cd86 percp cy5, by BioLegend (1 mentions)
Cd8a apc cy7, by BioLegend (1 mentions)
Rat anti cd11b bv711 clone m1 70, by BioLegend (1 mentions)
Rat anti cd45 percp cy5.5 clone 30 f11, by Thermo Fisher Scientific (1 mentions)
Cd11c pacblue, by BioLegend (1 mentions)
Attune nxt apparatus, by Thermo Fisher Scientific (1 mentions)
Cd40 pe, by BD (1 mentions)
2 protocols using rat anti cd45 v500 clone 30 f11
1
Immunophenotyping of Peritoneal Cells
2
Multicolor Flow Cytometry of Tumor Cells
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After blocking and live-dead staining, tumor-derived and cultured immune cells (BMMs, T cells and BMDCs) were stained with the following antibodies (all diluted 1:100) for 30 min at 4°C: rat anti-CD11b-BV711 (clone M1/70, BioLegend), rat anti-CD11b-PE-Cy7 (clone M1/70, eBioscience), rat anti-CD45-V500 (clone 30-F11, BD Biosciences), rat anti-CD45-PerCP-Cy5.5 (clone 30-F11, eBioscience), rat anti-F4/80-APC-Cy7 (clone BM8, BioLegend), rat anti-F4/80-AF647 (clone BM8, BioLegend), rat anti-Ly6G-BV605 (clone 1A8, BioLegend), rat anti-Ly6C-AF700 (clone HK1.4, BioLegend), CD3-APC (clone 17A2, BioLegend), CD8a-APC/Cy7 (clone 53-6.7, BioLegend), CD4-APC/eFluor780 (clone GK1.5, eBioscience), CD40-PE (clone: 3/23, BD Biosciences), CD86-PerCP-Cy5.5 (clone GL-1, BioLegend), CD80-FITC (clone 16-10A1, BioLegend), MHC II-AF647 (clone M5/114.15.2, BioLegend) and CD11c-PacBlue (clone N418, BioLegend). All samples were analyzed with an Attune NxT apparatus (Life Technologies). Compensation was performed using single-stained OneComp eBeads (Thermo Fisher Scientific).
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