Enzygnost anti measles virus igg

Manufactured by Siemens

Sourced in Germany

The Enzygnost® Anti-Measles Virus/IgG is a laboratory diagnostic test used to detect the presence of antibodies against the measles virus in human serum or plasma samples. The test utilizes an enzyme immunoassay (EIA) technique to measure the level of measles-specific IgG antibodies.

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Lab products found in correlation

Anti rubella virus igg, by Siemens (3 mentions) Enzygnost anti rubella virus igg, by Siemens (1 mentions)

7 protocols using enzygnost anti measles virus igg

Serological Testing Protocols for Measles

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Testing of the residual sera was conducted by the National Public Health Laboratory, the national reference laboratory for measles in Singapore. Immunoglobulin G (IgG) antibody levels were measured using an enzyme-linked immunosorbent assay (ELISA), Enzygnost® Anti-Measles Virus/IgG (Siemens, Germany). Test results were reported in corrected optical density (∆OD) and classified into three categories according to the manufacturer’s instructions: negative (∆OD < 0.1), equivocal (0.1 ≤ ∆OD ≤ 0.2) or positive (∆OD > 0.2).
Plaque reduction neutralization test (PRNT) is known as the gold standard assay for evaluation of humoral immunity to measles [17 , 18 (link)]. Samples with equivocal and negative ELISA results were further evaluated using PRNT. The assay used was adopted from a standardized laboratory protocol for measles PRNT by the WHO which was subsequently validated for use in clinical trials of aerosolized measles vaccines [19 ]. The measles PRNT titre was calculated from the dilution that reduced the number of plaques by 50%, and a value ≥ 8 was considered positive.

Ang L.W., Gao Q., Cui L., Farwin A., Toh M.P., Boudville I.C., Chen M.I., Chow A., Lin R.T., Lee V.J, & Leo Y.S. (2022). Prevalence of measles antibodies among migrant workers in Singapore: a serological study to identify susceptible population subgroups. BMC Infectious Diseases, 22, 88.

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Nationwide Sero-Prevalence Survey of Measles and Rubella in Lao PDR

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We used data collected during a nationwide multistage random cluster sampling survey in 2014 in Lao PDR, measuring anti-measles and anti-rubella IgG prevalence among children and adults, as described in^{4 (link)}. The survey collected blood samples from 2135 children and adults living in 52 randomly-selected villages drawn from all 143 districts of Lao PDR and was conducted for 2 weeks from 27 January to 7 February, 2014. The analyses were restricted to data for the age groups 5–21 years as people in this age range would have received MR vaccine in 2011. As described in^{4 (link)}, IgG levels were measured from dried blood spots on the filter paper using commercially available enzyme-linked immunosorbent assay (ELISA) kits (Enzygnost Anti-Measles Virus/IgG and Anti-Rubella Virus/IgG, Siemens Healthcare Diagnostics) according to the manufacturer’s instructions at the Department of Virology 3, National Institute of Infectious Diseases, Japan. Optical density values were converted to quantitative data, and the results were considered positive at higher than 120 mIU/mL for measles and 10 IU/mL for rubella^{14 (link),15}. Figure 2 summarises the observed data.

Vynnycky E., Miyano S., Komase K., Mori Y., Takeda M., Kitamura T., Xeuatvongsa A, & Hachiya M. (2019). Estimating the immunogenicity of measles-rubella vaccination administered during a mass campaign in Lao People’s Democratic Republic using multi-valent seroprevalence data. Scientific Reports, 9, 12545.

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Measles, Mumps, and Rubella Antibody Assays

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Laboratory tests were performed at the National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Bangkok, Thailand. The indirect ELISA using Enzygnost® Anti-Measles Virus/IgG, Anti-Parotitis-Virus/IgG, Anti-Rubella-Virus-IgG (Siemens, Marburg, Germany) were performed following the manufacturer’s instructions. The optical density (OD) readings were interpreted as negative, equivocal, and positive if the delta OD was <0.1 (cut off), 0.1–0.2, and >0.2, respectively. Positive delta ODs were then converted to international units, as described in the package insert [17 –19 ]. Protective antibody levels were defined as an antibody level ≥320 mIU/mL, antibody titer ≥1:500, and antibody level ≥10 IU/mL for measles, [10 ] mumps, [20 (link)] and rubella, respectively [21 (link)].

Chaiwarith R., Praparattanapan J., Nuket K., Kotarathitithum W, & Supparatpinyo K. (2016). Seroprevalence of antibodies to measles, mumps, and rubella, and serologic responses after vaccination among human immunodeficiency virus (HIV)-1 infected adults in Northern Thailand. BMC Infectious Diseases, 16, 190.

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Measles Immune Status Assessment

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To assess the immune status of the study participants against measles, IgG against measles was measured using Enzygnost^® Anti-Measles Virus/IgG (Siemens Healthcare Diagnostics Products, Germany). A 2-mL blood was collected under complete aseptic conditions. All steps were performed according to the enzyme-linked immunosorbent assay kit protocol. Photometric evaluation of the samples was performed using a measuring wavelength of 450 nm with Enzygnost^® Anti-Measles Virus/IgG. Samples containing approximately 150 mIU/mL were found to be within the range of 0.100 to 0.200 ΔA. Anti–Measles Virus/IgG negative ΔA < 0:100 (cutoff value). Anti–MeaslesVirus/IgGpositive ΔA > 0:200. HCWs with negative measles IgG were informed about the results and were offered immunization with either a measles vaccine or MMR vaccine according to the availability of the vaccine and the HCWs’ sex (MMR vaccines were used for women).

El-Sokkary R.H., Tash R.M., Zalat M.M., Arafa M, & Malek M.M. (2020). Healthcare Workers’ Preparedness: An Exploratory Study for Measles Control in a Middle-Income Country. Infection and Drug Resistance, 13, 395-402.

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Measles, Mumps, and Rubella Antibody Determination

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For measles/mumps/rubella antibody determination, commercial enzyme-linked immunosorbent assay (ELISA) kits were used: Enzygnost^® Anti-Measles Virus/IgG (Siemens Healthcare Diagnostics GmbH, Marburg, Germany); Enzygnost^® Anti-Mumps Virus/IgG (Siemens Healthcare Diagnostics GmbH, Marburg, Germany); Enzygnost^® Anti-Rubella Virus /IgG (Siemens Healthcare Diagnostics GmbH, Marburg, Germany). Despite the fact that a correlate of protection for mumps has not yet been found, and for measles and rubella, has only been identified in the microneutralization assay, the putative quantitative threshold for seropositivity considered here was ≥0.15 IU/mL for measles, a titer of ≥1:230 for mumps, and ≥4 IU/mL for rubella [29 (link)]. However, considering that antibody levels were defined as low positive (equivocal) at 0.15–0.35 IU/mL for measles, a titer of 1:230 to 1:500 for mumps, and 4–7 IU/mL for rubella, according to Davidkin et al. [29 (link)], the more restrictive limits of ≥0.35 IU/mL, 1:500, and ≥7 IU/mL, for measles, mumps, and rubella, respectively, were actually adopted as the putative thresholds for protection (Table 2). Responders were defined the subjects able to at least quadruplicate post-vaccination antibody levels [30 (link)], irrespective of the baseline antibody levels.

Ferlito C., Biselli R., Visco V., Cattaruzza M.S., Capobianchi M.R., Castilletti C., Lapa D., Nicoletti L., Marchi A., Magurano F., Ciccaglione A.R., Chionne P., Madonna E., Donatelli I., Calzoletti L., Fabiani C., Biondo M.I., Teloni R., Mariotti S., Salerno G., Picchianti-Diamanti A., Salemi S., Caporuscio S., Autore A., Lulli P., Borelli F., Lastilla M., Nisini R, & D’Amelio R. (2021). Immunogenicity of Viral Vaccines in the Italian Military. Biomedicines, 9(1), 87.

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Measles and Rubella Seroprevalence Survey

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Survey teams of two were organized in each selected district to conduct a brief face-to-face interview to obtain the demographic information and blood collection by finger prick [16] 16. Uzicanin, A • Lubega, I • Nanuynja, M ... Dried blood spots on filter paper as an alternative specimen for measles diagnostics: detection of measles immunoglobulin M antibody by a commercial enzyme immunoassay J Infect Dis. 2011; 204:S564-S569 Crossref Scopus (24) PubMed Google Scholar from the individuals sampled from two villages in the same district. A small amount of blood was put onto filter paper (Whatman 903®) using capillary tubes, dried well, and transported to the WHO Measles and Rubella Regional Reference Laboratory, as well as the Global Specialized Laboratory in the Department of Virology III, National Institute of Infectious Diseases (NIID), Tokyo, Japan, for the laboratory examinations. The antimeasles and antirubella IgG titers were measured using commercially available enzyme-linked immunosorbent assay kits (Enzygnost Anti-measles virus/IgG and Anti-rubella virus/IgG, Siemens Healthcare Diagnostics). The Optical Density (OD) values were converted to quantitative data, and we presented the results with cut-off points of 120 mIU/ml for antimeasles IgG that has been considered to be protective against symptomatic disease according to past studies [16] [17] [18]

Miyano S., Vynnycky E., Pattamavone C., Ichimura Y., Mori Y., Nouanthong P., Phounphenghack K., Tengbriacheu C., Khamphaphongphane B., Franzel L., Yang T.U., Raaijimarkers H., Komada K., Ota T., Funato M., Takeda M, & Hachiya M. (2023). Comparison of population-based measles-rubella immunoglobulin G antibody prevalence between 2014 and 2019 in Lao People's Democratic Republic: Impacts of the national immunization program. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 129.

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Measles Antibody Detection by ELISA

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After the blood samples were drawn, the serum was separated and stored frozen at -20 °C within at most 4 hours after collection and sent in a well-preserved cold-chain to the reference national measles laboratory in the School of Public Health, in Tehran University of Medical Sciences, Tehran.
In order to determine the presence of IgG antibodies against the measles virus, we used indirect enzyme-linked immunosorbent assay (ELISA), according to the manufacturer's instructions (Enzygnost® Anti-Measles Virus/IgG; Siemens). Based on the manufacturer's guidelines, samples containing approximately 150 mIU/mL were found to be within the optimal density range of 0.100-0.200 ∆A. Specimens below 0.100 ∆A were considered as negative.

Izadi S., Mokhtari-Azad T, & Zahraei S.M. (2015). Measles vaccination coverage and seroprevalence of anti-measles antibody in south-east Islamic Republic of Iran. Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit, 21(6).

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