TBA value of the fat extracted from milk fortified with WPC–Fe complex was evaluated by the method described by Sidwell et al. (21 (link)). The secondary oxidation products were analysed using spectrophotometer (UV-2700; Shimadzu Corporation, Kyoto, Japan). A mass of 3.0 g of molten fat was weighed accurately in a glass stoppered test tube. To this, 10.0 mL CCl4 and 10.0 mL TBA reagent (both from Sigma Aldrich, Merck) were added. The content was shaken (Spinix vortex shaker 3020; Tarsons Products Pvt. Ltd) vigorously at about 125 rpm for 4 min. Test tubes were left undisturbed to obtain two clear separated layers. A volume of 5.0 mL of aqueous layer was transferred to a test tube, and incubated in boiling water bath (SUB Aqua 18 Plus; Grant Instruments) for 30 min. The red coloured supernatant was decanted and its absorbance was measured spectrophotometrically (UV-2700; Shimadzu Corporation) at 532 nm in a 1-cm light path. All measurements were run in triplicates.
Uv 2700
Manufactured by Shimadzu
Sourced in Japan, China, United States
The UV-2700 is a high-performance UV-visible spectrophotometer designed for a wide range of applications in the laboratory. It features a dual-beam optical system, a wavelength range of 185 to 1,400 nm, and a photometric range of -4 to 4 Abs. The instrument provides accurate and reliable measurements, making it suitable for various analytical tasks.
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Lab products found in correlation
Zeenit 700p, by Analytik Jena (6 mentions)
S 4800, by Hitachi (4 mentions)
D8 advance, by Bruker (4 mentions)
Liqui toc 2, by Elementar (3 mentions)
Escalab 250xi, by Thermo Fisher Scientific (3 mentions)
Jem 2100f, by JEOL (3 mentions)
F 4700, by Hitachi (2 mentions)
Jsm 7500f, by JEOL (2 mentions)
Asap 2020, by Micromeritics (2 mentions)
Ht7700, by Hitachi (2 mentions)
Iraffinity 1, by Shimadzu (2 mentions)
Zetasizer nano z, by Malvern Panalytical (2 mentions)
Ion trap xct with 1100 lc, by Agilent Technologies (2 mentions)
Luna c18 2 reverse phase column, by Phenomenex (2 mentions)
S 4800 sem, by Hitachi (2 mentions)
Fluorolog 3, by Horiba (2 mentions)
Zetasizer nano zs90, by Malvern Panalytical (2 mentions)
Sub aqua 18 plus, by Grant Instruments (1 mentions)
Avance 3 400, by Bruker (1 mentions)
Kratos axima cfrplus, by Shimadzu (1 mentions)
161 protocols using uv 2700
1
Determination of TBA Value in Fortified Milk Fat
2
Quantification of Lipid Peroxidation in Sunburned Fruits
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The measurement of lipid peroxidation in the sunburned fruits was analyzed using the MDA assay according to the method described by Jakhar and Mukherjee [52 (link)]. The peels of the sunburned or non-sunburned fresh fruits were separated and frozen in liquid nitrogen. Fresh samples (50 mg) were ground into a powder using liquid nitrogen, mixed with 3 mL of 50 mM phosphate buffer (pH 7.0), and centrifuged at 15,000× g for 15 min at 4 °C. A one microliter aliquot of appropriately diluted sample was then added to a tube with 2 mL of 0.5% thiobarbituric acid in 20% trichloroacetic acid. All reagents used were obtained from Sigma-Aldrich (Saint Louis, MO, USA). The mixture was heated in a water bath for 30 min at 95 °C and then rapidly cooled in an ice bath. The samples were centrifuged at 10,000× g for 10 min at 4 °C. The absorbance of the supernatants was recorded at 532 nm and 600 nm using a UV spectrophotometer (UV-2700; Shimadzu Corp., Seoul, Korea). The concentration of MDA was calculated using an extinction coefficient of 155 mM−1 cm−1.
3
Spectrophotometric Analysis of Organic Compounds
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Full-wavelength scanning was performed using a UV/Vis spectrophotometer (UV-2700; Shimadzu, Nakagyo-ku, Kyoto, Japan). An ethanol/water solution (50% v/v) was used to dissolve the standards, including lactic acid, propanoic acid, hexanoic acid, ethanol, methanol, pentanol, n-propanol, ethyl lactate, ethyl acetate, and ethyl formate, at a concentration of 10 µL/mL. Full-wavelength scanning of the standard solutions and collected samples was performed with a wavelength scanning range of 185–1400 nm. Samples (750 µL) were aspirated into a 5 mm diameter cuvette. After full-wavelength scanning, the maximum absorption values of the characteristic peaks (185–200 nm, 200–220 nm, 220–250 nm, and 250–300 nm) corresponding to the acid, ester, and peptide were processed for correlation analysis.
4
Quantitative Drug Dissolution in Oils
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An accurately measured quantity of drug was added in increasing concentrations and mixed in various oils and stirred until it dissolved completely. The solution was diluted with a methanol/chloroform mixture equally and the content of dissolved drug was determined using a UV spectrophotometer (Shimadzu UV-2700, Shimadzu, Kyoto, Japan) at 270 nm [35 (link)].
5
Doxorubicin-Loaded Lanthanide-Functionalized Mesoporous Silica Nanoparticles
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For the loading of DOX on the Ln-MCM-41 nanoparticles, 480 mg of the Ln-MCM-41 nanoparticles were dispersed in 80 mL phosphate buffer solution (PBS, pH = 7.4) with 40 mg DOX. After stirring for 2 h at room temperature, 3 mL of PA solution (5.0 mg mL−1) was added and stirred for another 30 min. Subsequently, the pH value of the mixture was adjusted to alkaline. After then, 0.5 mL of 0.24 M (NH4)3PO4·3H2O solution and 0.5 mL of 0.29 M Ca(NO3)2·4H2O solution were injected 8 times in turn at 10 min intervals. The as-fabricated product was collected by centrifugation at 10 000 rpm for 8 minutes and then freeze-dried under vacuum for 12 h dried at 60 °C, and the solid was named as DOX-Ln-MCM-41. UV-vis was used to determine the concentration of the separated supernatant solution. To evaluate the DOX-release behavior, 30 mg DOX-Ln-MCM-41 samples were immersed in 10 mL PBS solution (pH = 7.4, 6.5, or 5.5) at 37 °C under continuous stirring. At predetermined time intervals, 3 mL of the released medium was taken and centrifuged. The amount of released DOX was measured by a UV-vis spectrophotometer (Shimadzu UV-2700, Japan) at a wavelength of 480 nm. Finally, the medium taken out for measurement was returned to the original released medium.
6
Synthesis of GO/AuNPs Hybrid Nanocomposites
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20 mL 0.2 g·L−1 GO solution was added to 20 mL 30% H2O2 solution at room temperature, followed by stirring for 4 h under ultraviolet light excitation (Portable ultraviolet radiation light, WFH-204B, 254 nm, 50 HZ). The resulting 3.5 mL GO solution were mixed with 350 μL 1 wt % aqueous sodium citrate and 40 mL distilled water in a 100 mL Erlenmeyer flask. With 800 rpm vigorous stirring the solution was heated to reflux, and 500 μL 1 wt % HAuCl4 was added within 2 s. The solution remained under reflux for another 10 min, then the heating source was removed while the solution was kept continuously stirring and cooled down to ambient temperature within 10 min. The resultant GO/AuNPs hybrids were washed twice by distilled water after centrifugation at 2800 rpm for 5 min (DAIHAN WiseSpin CF-10), and were stored at 4 °C for no longer than four weeks (the storage time on SERS response is shown in Fig. S1, Supporting Information ). The GO/AuNPs were characterized by a transmission electron microscopy (TEM) (Hitachi H-7500) and a UV-Vis spectrometer (Shimadzu UV-2700). Before use, the GO/AuNPs were gently vortexed for 1 min (the effect of vortexing time on SERS response is shown in Fig. S2, Supporting Information ).
7
Spectroscopic Characterization of Organic Compounds
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The nuclear magnetic resonance (NMR) spectra were obtained using a Bruker AVANCE III 400 (400 MHz). The 1H NMR and 13C NMR spectra were measured with TMS as the internal standard. The matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry data were obtained using a Shimadzu Kratos AXIMA-CFR Plus in the linear positive ion mode. Dithranol was used as the matrix. The UV-vis spectra were recorded using a Shimadzu UV-2700 with a quartz cell (optical length, 1 cm) at 20°C in a glove box. The elemental analysis was performed at the Center for Advanced Materials Analysis, Technical Department, Tokyo Institute of Technology. The molecular models were calculated with SCIGRESS v2.1.0 (Fujitsu) using the MM/AM1 MOZYME method and with Gaussian 09, Revision C.01 (46 ) on the nodes of a supercomputer (TSUBAME2, Tokyo Institute of Technology). A preparative scale gel permeation chromatograph, LC-908 (Japan Analytical Industry Co. Ltd.), was used to isolate each compound with chloroform as the eluent.
8
Soil Physicochemical and Plant Biomass Analyses
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Soil pH was measured by pH meter (STARTER3100, Ohaus Instruments Co., Ltd, Shanghai, China) with a 1:5 of soil water ratio (5 g soil: 25 mL ddH2O). SWC was measured by ovening fresh soil samples at 105°C for 24 hr. SOC was measured by a TOC analyzer (Liqui TOC II; Elementar Analysensysteme GmbH, Hanau, Germany). Soil TN was measured on an auto-analyzer (SEAL Analytical GmbH, Norderstedt, Germany). Soil TP and AP were measured by a UV-VIS spectrophotometer (UV2700, SHIMADZU, Japan). Nitrate-N (NO3-) and ammonium-N (NH4+) were extracted with 2 M KCl (soil mass to solution ratio of 1:5) and then analyzed on a continuous-flow ion auto-analyzer (SEAL Analytical GmbH, Norderstedt, Germany). Soil DOC and DON were measured on a TOC Analyser (Liqui TOC II; Elementar Analysensysteme GmbH, Hanau, Germany). Plant AGB and BGB were measured after oven drying at 65°C for 72 hr. MAT (1980–2014) and MAP (1980–2014) of each study site were obtained from ‘China Meteorological Data Service Center’ (CMDC: https://data.cma.cn/ ) by latitude and longitude.
9
UV-Vis Characterization of AgNPs, MFP, and MFP@AgNPs
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AgNPs, MFP and MFP@AgNPs were diluted with water and the UV-vis absorbance of the samples was recorded via UV-vis spectroscopy (UV-2700, Shimadzu, China).
10
Characterization of Nanomaterials
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UV-visible spectroscopy was measured using a Shimadzu UV-2700 equipped with an integrated sphere. XRD patterns were measured using a modified Bruker-Axs D8 diffractometer with parallel beam optics equipped with a PSD LinxEye silicon strip detector. The instrument uses a Cu source for X-ray generation (V = 40 kV, I = 30 mA) with Cu Kα1 (λ = 1.54056 Å) and Cu Kα2 radiation (λ = 1.54439 Å) emitted with an intensity ratio of 2 : 1. The incident beam was kept at 1° and the angular range of the patterns collected between 10 ≤ 2θ° ≤ 66 with a step size of 0.05°. SEM images were obtained with a LEO 1525 scanning electron microscope (FESEM, 5 kV).
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