I class acquity uplc system

Manufactured by Waters Corporation

Sourced in United Kingdom

The I Class Acquity UPLC system is a high-performance liquid chromatography (HPLC) instrument designed for efficient and precise separation and analysis of chemical compounds. It utilizes ultra-high-pressure liquid chromatography (UPLC) technology to deliver improved resolution, sensitivity, and speed compared to traditional HPLC systems.

Automatically generated - may contain errors

Lab products found in correlation

Xevo tqd triple quadrupole mass spectrometer, by Waters Corporation (2 mentions) Sequant zic hilic column, by Merck Group (1 mentions) Vion ims qtof system, by Waters Corporation (1 mentions) Simca 14, by Sartorius (1 mentions) Analyst software, by AB Sciex (1 mentions) Multiquant 3, by AB Sciex (1 mentions) Cobas 6000 analyzer, by Roche (1 mentions) Masslynx 4, by Waters Corporation (1 mentions) Xevo tq s triple quadrupole mass spectrometer, by Waters Corporation (1 mentions) Acquity uplc beh c18 column, by Waters Corporation (1 mentions)

6 protocols using i class acquity uplc system

HILIC LC-MS/MS for Ado and 2'-dAdo Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

A < 5 min LC-MS/MS method for the separation of Ado and 2′-dAdo from their metabolites, Ino and 2′-dIno, was developed on a Waters I Class Acquity UPLC system. Sample detection and quantification was performed on a Waters Xevo TQD triple quadrupole mass spectrometer in positive electrospray mode as defined above. 10 µL of sample prepared as described in section 2.4, or sample mixtures prepared from liquid calibrators, were injected onto a Supelcosil^TM LC-NH₂ 2.1 × 50 mm, 3 μm HILIC column (Sigma Aldrich, St. Louis, MO) for separation. The samples were eluted with a flow rate of 0.4 mL/min before a quick column wash at 1.2 mL/min. See Table 2 for mobile phase and gradient conditions.Table 2

Mobile phase and gradient conditions in HILIC LC-MS/MS method specific to ADA-SCID. Mobile phase (A): 97:3 acetonitrile/water, with 1% formic acid and mobile phase (B): 100% water with 1% formic acid.

Time (min)	Flow rate (mL/min)	% A	% B
0.00	0.400	100	0
1.00	0.400	100	0
2.50	0.400	93	7
4.00	0.400	88	12
4.10	0.400	100	0
5.00	1.20	100	0
5.20	0.400	100	0

Young B., Hendricks J., Foreman D., Pickens C.A., Hovell C., De Jesús V.R., Haynes C, & Petritis K. (2020). Development of dried blood spot quality control materials for adenosine deaminase severe combined immunodeficiency and an LC-MS/MS method for their characterization. Clinical Mass Spectrometry, 17, 4-11.

+ Open protocol

+ Expand

HPLC-MS/MS for Adenosine Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

A < 5 min HPLC method for the separation of Ado and 2′-dAdo from their metabolites, Ino and 2′-dIno, was developed on a Waters I Class Acquity UPLC system. Sample detection and quantification was performed on a Waters Xevo TQD triple quadrupole mass spectrometer in positive electrospray mode as defined above. 10 μL of sample prepared as described in section 2.4, or sample mixtures prepared from liquid calibrators, were injected onto a Supelcosil^™ LC-NH₂ 2.1 × 50 mm, 3 μm HILIC column (Sigma Aldrich, St. Louis, MO) for separation. The samples were eluted with a flow rate of 0.4 mL/min before a quick column wash at 1.2 mL/min. See table 2 for mobile phase and gradient conditions.

+ Open protocol

+ Expand

HILIC-MS Metabolite Profiling Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dried metabolite extracts were resuspended in 50 μL of methanol–water (1:1, v/v) and analyzed using a method as described previously [51 (link)]. Metabolite separation was achieved by a SeQuant ZIC-HILIC column (100 mm × 2.1 mm i.d., 3.5 µm) (Merck, Germany) using a Waters I-Class Acquity UPLC system (Waters, UK). The UPLC system was coupled to a Vion IMS QToF system (Waters, UK) [51 (link)].
Significant differences were analyzed by a two-tailed Student’s t test with Microsoft Excel 2016. The principal component analysis plots were generated using SIMCA 14.1 (Umetrics, Umea, Sweden). PCA was applied to the data after mean centering and UV scaling.

Yao R., Li J., Feng L., Zhang X, & Hu H. (2019). 13C metabolic flux analysis-guided metabolic engineering of Escherichia coli for improved acetol production from glycerol. Biotechnology for Biofuels, 12, 29.

+ Open protocol

+ Expand

Highly Sensitive Urinary 2CyEMA Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used ultra-high-performance liquid chromatography (I-Class Acquity UPLC system, Waters Inc., Milford, MA) coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS; Sciex 5500 Triple quad, Sciex, Framingham, MA) for the measurement of urinary 2CyEMA.^{22 (link)} The details about the experimental workflow are described elsewhere.^{22 (link)} Briefly, the chromatographic separation was achieved using an Acquity UPLC® HSS T3, 100 Å, 1.8 μm, 2.1mm × 150 mm column (Waters Inc., Milford, MA) with a Waters HSS T3 VanGuard pre-column (Waters Corporation, Milford, MA). The mass spectrometer was operated in negative ion ESI scheduled multiple reaction monitoring mode. Data was acquired using Analyst software (Sciex, Framingham, MA), and processed in MultiQuant 3.0.3 (Sciex, Framingham, MA). Sample concentrations were determined based on their relative response ratio (ratio of native analyte to stable isotope-labeled internal standard) against a calibration curve with known standard concentrations. The limit of detection (LOD) was 0.5 ng/mL.
Serum cotinine was measured by an isotope dilution HPLC-APCI-MS/MS method, and creatinine was measured using Enzymatic Roche Cobas 6000 Analyzer. A detailed description of the laboratory methodologies can be found elsewhere.²³

Bhandari D., Zhang L., Zhu W., De Jesús V.R, & Blount B.C. (2022). Optimal Cutoff Concentration of Urinary Cyanoethyl Mercapturic Acid for Differentiating Cigarette Smokers from Nonsmokers. Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco, 24(5), 761-767.

+ Open protocol

+ Expand

FIA-ESI-MS/MS Assay Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

During FIA, an in-line stainless steel filter (0.094 × 0.065 in, 2 μm) was used with a Waters I Class Acquity UPLC system (Waters Corporation, Milford Massachusetts) with a mobile phase composed of 50:50 acetonitrile/water with 0.02% formic acid. A 10 μL injection of the sample was analyzed. Total run time was 1.0 min with elution of the analytes at 0.50 minutes and maximum typical back pressure of approximately 300 psi. The initial flow rate was 100 μL/min; at 0.25 min flow was decreased to 20 μL/min; at 0.75 min flow was increased to 600 μL/min; and between 0.85- and 1.00-min flow was decreased to 100 μL/min. This inlet program is identical to one used during a typical AA, AC, and SUAC FIA-ESI-MS/MS assay [22 (link)].

+ Open protocol

+ Expand

Quantitative LC-MS Analysis of CoQ Redox State

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

CoQ redox state determination by LC-MS was performed as described previously^{26 (link)}. LC-MS/MS analyses were carried out using an I-Class ACQUITY UPLC^® system attached to a Xevo TQ-S triple quadrupole mass spectrometer (both Waters, UK). Samples were kept at 8 °C prior to injection by the autosampler of 2–10 μL into a 15 μL flow-through needle and separated by reverse-phase at 45 °C using an ACQUITY UPLC^® BEH C18 column (1.7 μM, 130 Å, 2.1 × 50 mm; Waters, UK). Mobile phase was isocratic 2 mM ammonium formate in methanol run at 0.8 mL/min over 5 min. For MS analysis, electrospray ionization in positive ion mode was used with the following settings: capillary voltage 1.7 kV; cone voltage 30 V; ion source temperature 100 °C; collision energy 22 V. Multiple reaction monitoring in positive ion mode was used for compound detection. Transitions used for quantification were: CoQ₉, 812.9 > 197.2; CoQ₉H₂, 814.9 > 197.2. Samples were quantified using MassLynx 4.1 software (Waters, UK) to determine the peak areas for CoQ₉ and CoQ₉H₂.

Yin Z., Burger N., Kula-Alwar D., Aksentijević D., Bridges H.R., Prag H.A., Grba D.N., Viscomi C., James A.M., Mottahedin A., Krieg T., Murphy M.P, & Hirst J. (2021). Structural basis for a complex I mutation that blocks pathological ROS production. Nature Communications, 12, 707.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!