Sirna oligonucleotide

Manufactured by GenePharma

Sourced in China

SiRNA oligonucleotides are short, double-stranded RNA molecules designed to silence the expression of specific genes. They function by binding to and degrading target messenger RNA (mRNA), thereby preventing the translation of the corresponding protein.

Automatically generated - may contain errors

Lab products found in correlation

143 protocols using sirna oligonucleotide

Silencing RAB11FIP5 in iSLK.RGB Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two siRNA oligonucleotides targeting RAB11FIP5 and the corresponding negative control siRNA oligonucleotide were obtained from GenePharma. The sequences were as follows: siRAB11FIP5-#1, 5′-GGUACAAGCUGCACUCCAATT-3′; siRAB11FIP5-#2, 5′-GCAAGAUGGGCAAAGCCAATT-3′. The siRNA oligonucleotides were transfected into iSLK.RGB cells with Lipofectamine 2000 in accordance with the manufacturer’s instructions.

Wei X., Dong J., Cheng C.C., Ji M., Yu L., Luo S., Wu S., Bai L, & Lan K. (2020). Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45. PLoS Pathogens, 16(12), e1009099.

+ Open protocol

+ Expand

HMGB3 Silencing in BRCA Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were transfected using a liposome delivery system (Cat. # 11668027, Thermo Fisher Scientific). For the transfection of small interfering RNA (siRNA), siRNA oligonucleotides targeting HMGB3 and nonspecific siRNA oligonucleotides were purchased from GenePharma (China): 5′-GGUCUUCGCCUUGAUUCAUTT-3′ and 5′-AUGAUAUAAGGCGAAGACCTT-3′. The siRNA oligonucleotides were transfected into BRCA cells using liposomes according to the standard protocol provided by the manufacturer. Forty-eight hours after transfection, cells were harvested and analyzed by western blotting or used in further experiments.

Zhou X., Zhang Q., Liang G., Liang X, & Luo B. (2022). Overexpression of HMGB3 and its prognostic value in breast cancer. Frontiers in Oncology, 12, 1048921.

+ Open protocol

+ Expand

Knockdown and Overexpression of GAS5 in MEG-01 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Oligonucleotides of 3 GAS5‐specific siRNAs (GAS5 si‐1, GAS5 si‐2, and GAS5 si‐3) were used to knock down GAS5 referring to the study by Houqi Liu et al.,⁴⁵ and the non‐silencing control siRNA oligonucleotide was used as a negative control (GenePharma, Shanghai, China). GAS5 cDNA was amplified by PCR and subcloned into GV219 to generate the overexpression vector named GV219‐GAS5 (Genechem, Shanghai, China), with the empty GV219 vector (GV219‐NC) as a control. The miR‐223‐3p mimic, inhibitor, and the corresponding negative controls were synthesized by Ribobio (Guangzhou, China); 4×10⁵/well of MEG‐01 cells were plated into a 6‐well plate, and plasmids were transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA) following the manufacturer's protocol. Lipofectamine RNAiMAX (Thermo Fisher Scientific, MA, USA) was used to transfect GAS5 siRNAs and the miR‐223‐3p mimic/inhibitor following the manufacturer's instructions. The cells were harvested 48 hours after transfection for further analysis. GAS5‐specific siRNA sequences and miR‐223‐3p mimic or inhibitor sequences are listed in Table S1.

Liu Y., Hu X., Song P., Li H., Li M., Du Y., Li M., Ma Q., Peng L., Song M, & Chen X. (2021). Influence of GAS5/MicroRNA‐223‐3p/P2Y12 Axis on Clopidogrel Response in Coronary Artery Disease. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 10(21), e021129.

+ Open protocol

+ Expand

Mitochondrial Dynamics Regulation in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human TFAM shRNA (shTFAM) constructs were purchased from OriGene (Rockville, MD, USA). Cells with stable knockdown of TFAM were isolated by transfecting the shTFAM construct and then performing selection under the presence of puromycin. The siRNA oligonucleotide targeting human DRP1 (GCUACUUUACUCCAACUUAUUTT) was synthesized in GenePharma (Shanghai, China). To knock down DRP1, the cells were grown to 70% confluence in a cell culture dish. Then, the DRP1 siRNA was mixed with Lipofectamine 2000 (Thermo Fishier, Carlsbad, CA, USA) and added into the cell culture. After incubation for 48 h, the cells were subjected to radiation treatment and analysis.

Tang F., Zhang R, & Wang J. (2019). Cyclooxygenase-2-Mediated Up-Regulation of Mitochondrial Transcription Factor A Mitigates the Radio-Sensitivity of Cancer Cells. International Journal of Molecular Sciences, 20(5), 1218.

+ Open protocol

+ Expand

Silencing α5-nAChR mRNA via siRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

A siRNA oligonucleotide targeting α5-nAChR mRNA was synthesized by Shanghai Genepharma Co. Ltd. The sequence was as follows: 5′-CCCGCAAACUACAAAAGUUTT-3′. A pair of scrambled control siRNAs with sequences different from those of the siRNA-α5-nAChR was designed. The pair of sequences was not homologous to any sequences found in GeneBank. When the cells reached 70–80% confluence, the transfection was conducted according to the transfection instructions. The cells were subsequently cultured under normal conditions for 36 h at 37°C.

Qi J.C., Xue W.Y., Zhang Y.P., Qu C.B., Lu B.S., Yin Y.W., Liu K.L., Wang D.B., Li W, & Zhao Z.M. (2019). Cholinergic α5 nicotinic receptor is involved in the proliferation and invasion of human prostate cancer cells. Oncology Reports, 43(1), 159-168.

+ Open protocol

+ Expand

Targeted siRNA for Annexin A2 Isoform 2

Check if the same lab product or an alternative is used in the 5 most similar protocols

The siRNA oligonucleotide targeting Annexin A2 isoform 2 was designed and synthesized by Gene Pharma (Shanghai, China) based on the published sequence of Annxin A2 isoform 2 (sense 5′-GCAUAGCAACUUCGGAUUUTT-3′; antisense 5′-AAAUCCGAAGUUGCUAUGCTT-3′). Meanwhile, siRNA for negative control (sense 5′-UUCUCCGAACGUGUCACGUTT-3′; antisense 5′-ACGUGACACGUUCGGAGAATT-3′) was synthesized.

Gu J., Ma Y., Yang L., Wang F., Lei C., Zhai J, & Xia R. (2018). Role of Annexin A2 isoform 2 on the aggregative growth of dermal papillae cells. Bioscience Reports, 38(6), BSR20180971.

+ Open protocol

+ Expand

Cell Line Culture and siRNA Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five OS cell lines, Saos-2, SJSA-1, MG63, HOS, and U-2 OS, and the human osteoblast cell line, human fetal osteoblastic (hFOB) 1.19 cells, were obtained from the American Type Culture Collection (ATCC). Cells were cultured in DMEM medium (Cat# SH30243.01, HyClone™, Cytiva, Marlborough, MA, USA), RPMI medium 1,640 (Cat# 11875093, Thermo Fisher Scientific, Waltham, MA, USA), and DMEM/F12 medium (Cat# D9785, Sigma). All media were supplemented with 10% fetal bovine serum (FBS) (Cat# 04-001-1ACS; BI) and 1% penicillin/streptomycin (Cat# C0222, Beyotime, Shanghai, China). OS cell lines were maintained at 37°C in 5% CO₂, whereas hFOB 1.19 cells were cultured under 3°C.
The small interfering RNA (si-RNA) oligonucleotide targeting lncRNA DANCR and the matched negative control were generated by GenePharma (Shanghai, China). Lipofectamine 2000 (Cat# 11668019; Thermo Fisher Scientific) for transfections was used according to the manufacturer’s protocols.

Zhou X., Yang Y., Li Y., Liang G., Kang D., Zhou B, & Li Q. (2022). METTL3 Contributes to Osteosarcoma Progression by Increasing DANCR mRNA Stability via m6A Modification. Frontiers in Cell and Developmental Biology, 9, 784719.

+ Open protocol

+ Expand

siRNA-Mediated Knockdown of E3 Ligase Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were transfected with siRNA oligonucleotides synthesized by Genepharma (Shanghai, China) using Lipofectamine 2000. The sequences of the siRNA were as follows: RBX1, 5′-GACTTTCCCTGCTGTTACCTAATT-3′, 5′-CTGTGCCATCTGCAGGAACCACATT-3′; Cullin1, 5′-CUAGAUACAAGAUUAUACAUGCGG-3′; Cullin2, 5′-GCACAAUGCCCUUAUUCAA-3′, 5′-GCAGAAAGACACACCACAA-3′; Cullin3, 5′-TTGACGTGAACTGACATCCACATTC-3′, 5′-TACATATGTGTATACTTTGCGATCC-3′; Cullin4A, 5′-GAAGAUUAACACGUGCUGGTT-3′; Cullin4B, 5′-AAGCCUAAAUUACCAGAAA-3′, 5′-GGAGUUAUUUAGGGCUCAU-3′; Cullin5, 5′-CUACUGACUCUGAGAAAUA-3′, 5′-GAGCAAAUAGAGUGGCUAA-3′; Cullin7, 5′-UGAGAUCCUAGCUGAACUG-3′, 5′-UGUCCAAGGAUGAGAUCUA-3′; EloB, 5′-UGAACAAGCCGUGCAGUGA-3′, 5′-AGCGGCUGUACAAGGAUGA-3′; Prame, 5′-CCUGGAAGCUACCCACCUUTT-3′, 5′-GCUCCCAGCUUACGACCUUTT-3′; LRR1, 5′-CCUGUGGAUAUCUGUCUAATT-3′, 5′-GCUCUCAUAUCAUUCCAUUTT-3′; FEM1B, 5′-GCCCGCAAUGGACACGCAATT-3′, 5′-CCUAAUGAUUGCGGCAUAUTT-3′; VHL, 5′-GCCAGUGUAUACUCUGAAATT-3′, 5′-GCUCUACGAAGAUCUGGAATT-3′, 5′-TTGTGCCATCTCTCAATGTTGAC-3′.

Zhang W., Li L., Cai L., Liang Y., Xu J., Liu Y., Zhou L., Ding C., Zhang Y., Zhao H., Qin J., Shao Z., Wei W, & Jia L. (2021). Tumor-associated antigen Prame targets tumor suppressor p14/ARF for degradation as the receptor protein of CRL2Prame complex. Cell Death and Differentiation, 28(6), 1926-1940.

+ Open protocol

+ Expand

Silencing TCF21 in Chicken Preadipocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Synthetic small interfering (si) RNA oligonucleotides specific to the chicken TCF21 mRNA regions were synthesized by GenePharma (Shanghai, China). The siRNA sequences was: 5′-GGAAATGCTGGAGTGCGAT-3′. The negative control siRNA sequence was: 5′-TTCTCCGAACGTGTCACGT-3′. After 36 h of differentiation, preadipocytes were transfected with these siRNA constructs using Lipofectamine RNAi MAX (Invitrogen) based on the manufacturer’s protocols. Cells were maintained in differentiation media until day 3 post-transfection.

Zhang X., Cheng B., Liu C., Du Z., Zhang H., Wang N., Wu M., Li Y., Cao Z, & Li H. (2019). A Novel Regulator of Preadipocyte Differentiation, Transcription Factor TCF21, Functions Partially Through Promoting LPL Expression. Frontiers in Physiology, 10, 458.

+ Open protocol

+ Expand

Bora Silencing with siRNA Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

siRNA oligonucleotides targeting Bora and non-targeting siRNA were purchased from GenePharma. Transfections with siRNA were performed with Lipofectamine 2000. The siRNAs against Bora were (1) 5′-CTATGAGACTTCAGATGTA-3′ and (2) 5′-TAACTAGTCCTTCGCCTAT-3′. The control siRNA (siNC) was 5′-TTCTCCGAACGTGTCACGGTT-3′.

Zhang Q.X., Gao R., Xiang J., Yuan Z.Y., Qian Y.M., Yan M., Wang Z.F., Liu Q., Zhao H.D, & Liu C.H. (2017). Cell cycle protein Bora serves as a novel poor prognostic factor in multiple adenocarcinomas. Oncotarget, 8(27), 43838-43852.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!