Cx3cr1gfp gfp

C57BL/6, 7B8Tg, zDC-DTR, CCR7^−/−, CCR2^−/−, BATF3^−/−, CX3CR1^gfp/gfp, IL-6^−/−, MHCII^fl/fl, STAT3^fl/fl, IL-6Rα^fl/fl, CD4^Cre, Lysm^Cre, CD11c^Cre mice were purchased from The Jackson Laboratory. Mice were bred and maintained at the specific pathogen free animal facilities of the Medical University of South Carolina and later at the Ohio State University. All experiments were performed under protocols approved by Institutional Animal Care and Use Committee at both Universities. Mouse colonies were screened regularly for the presence of fecal SFB. Mice contaminated with SFB was treated with ampicillin in drinking water (1g/ml) for 2 weeks and let recovered before being used for experiments. SFB donor mice include C57BL/6 mice sourced from Taconic and in house SFB⁺ mice.

Experiments were conducted according to protocols approved by a local Institutional Animal Care and Use Committee and adhered to the Association for Research in Vision and Ophthalmology (ARVO) Statement for animal use in ophthalmic and vision research. Mice homozygous for the Pde6b^rd10 loss-of-function point mutation (rd10; Stock No. 004297) and for the loss-of-function CX3CR1-GFP targeted mutation (CX3CR1^GFP/GFP; Stock No. 005582) were obtained from The Jackson Laboratory (Bar Harbor, ME). Animals were genotyped and confirmed to lack the rd8 mutation (Mattapallil et al. 2012 (link)). Transgenic mouse lines were crossed together to generate Pde6b^rd10/rd10, CX3CR1^GFP/GFP mice; these were subsequently crossed with Pde6b^rd10/rd10, CX3CR1^+/GFP to generate Pde6b^rd10/rd10, CX3CR1^GFP/GFP and Pde6b^rd10/rd10, CX3CR1^+/GFP littermates (hereafter referred to as rd10;CX3CR1^GFP/GFP and rd10;CX3CR1^GFP/+ respectively). Experiments involved animals in the range of ages from postnatal day (P)15-P28 that were of mixed gender. Animals were housed in a National Institutes of Health animal facility under a 12-hour light/dark cycle with food ad libitum.

Both male and female C57BL/6N mice were used in accordance with the institutional guidelines, as approved by the Animal Care and Use Committee at Rutgers University. Homozygous GFP reporter mice (CX3CR1^GFP/GFP) expressing GFP under the control of the fractalkine receptor (CX3CR1) promoter (Jung et al., 2000 (link)) were obtained from Jackson Laboratory. The CX3CR1^GFP/- mice were used throughout the study to identify miocroglia in brain slices. For simplicity, we named CX3CR1^GFP/- mice as wild-type in this study. P2Y12R knockout (P2Y12 KO) mice were obtained from Dr. Michael Dailey at the University of Iowa. The CX3CR1^GFP/- P2Y12 KO was obtained by mating the above-mentioned mouse lines.