Ethanediamine (EDA, 99%), formaldehyde (30–40%), ethyl alcohol (99.7%), 3-aminophenol (99%), ethylenediamine tetraacetic acid disodium salt (EDTA·2Na, 99%), H2SO4 (95–98%), HCl (36–38%), NH3·H2O (25–28%), and BaCl2·2H2O (99.5%) were purchased from Sinopharm Chemical Reagent (Shanghai, China). NaOH (96%) and Na2SO4 (99%) were purchased from Shanghai Dahe Chemicals (Shanghai, China). NaH2PO4·2H2O (99%), and Na2HPO4·12H2O (99%) were purchased from Chinasun Specialty Product (Changshu, China). Tetraethoxysilane (TEOS, 99%), catalase (from bovine liver, 2000–5000 units/mg protein), 3,3′,5,5′-tetramethylbenzidine (TMB, 98%), and peroxidase from horseradish (HRP, ~200 units/mg) were purchased from Sigma–Aldrich (St. Louis, MO, USA). All chemicals were used without further purification.
Ethylenediaminetetraacetic acid disodium salt
Manufactured by Sinopharm
Sourced in China
Ethylenediaminetetraacetic acid disodium salt is a chemical compound commonly used as a chelating agent in various laboratory applications. It is a white crystalline powder that is highly soluble in water.
Automatically generated - may contain errors
Lab products found in correlation
Potassium ferricyanide, by Sinopharm (3 mentions)
Sodium hydroxide (naoh), by Sinopharm (2 mentions)
Trichloroacetic acid, by Sinopharm (2 mentions)
Tetraethoxysilane, by Merck Group (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions)
Catalase from bovine liver, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Sinopharm (1 mentions)
Peroxidase from horseradish, by Merck Group (1 mentions)
H2so4, by Sinopharm (1 mentions)
Formaldehyde, by Sinopharm (1 mentions)
Ethanediamine, by Sinopharm (1 mentions)
3 aminophenol, by Sinopharm (1 mentions)
Bacl2 2h2o, by Sinopharm (1 mentions)
Ethyl alcohol, by Sinopharm (1 mentions)
Nh3 h2o, by Sinopharm (1 mentions)
Sodium tripolyphosphate, by Sinopharm (1 mentions)
Bacl2, by Sinopharm (1 mentions)
Agno3, by Sinopharm (1 mentions)
Cck 8, by Merck Group (1 mentions)
Sodium hydroxide naoh, by Nanjing Chemical Reagent (1 mentions)
9 protocols using ethylenediaminetetraacetic acid disodium salt
1
Synthesis of Silica-based Biosensor Materials
2
Characterization of Potassium Sulfate Fertilizer
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Agricultural potassium sulfate (LN K2SO4) was obtained from SDIC Xinjiang Lop Nur Potash Co. Ltd., China. Chemical grade polyethylene glycol 1000 (PEG 1000), sodium tripolyphosphate (STPP), and analytical grade reagents for gravimetric methods and Volhard method (sodium tetraphenylborate, ethylenediaminetetraacetic acid disodium salt, ammonium iron sulfate, BaCl2 and AgNO3) were purchased from Sinopharm Chemical Reagent Co. Ltd. Industrial grade urea phosphate (UP) was purchased from Tianjin Kemiou Chemical Reagent Co. Ltd. Tap water was used in all experiments and its physicochemical properties are shown in Table 1 . The concentration of metal ions (K+, Na+, Mg2+, Ca2+) was determined by an atomic absorption spectrophotometer (AAS, AA-6880 SHIMADZU, Japan). The concentration of SO42− and Cl− anions was determined by the barium chloride gravimetric method and the Volhard method, respectively.
3
Hydrogel-Based Tissue Engineering Methodology
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Photoinitiator 1173 (HMPP), PEGDA, alginate (low viscosity, Alfa Aesar), calcium chloride (CaCl2), calcein-AM/PI (CA-PI), ALP, pepsin, pancreatin, potassium dihydrogen phosphate and phosphate buffer saline (PBS) were bought from Aladdin. LPS-fluorescein isothiocyanate (FITC) and CCK8 were bought from sigma. Hydrochloric acid (HCl) was bought from Nanjing Wanqing Chemical Glass ware and instrument Co., Ltd. Sodium hydroxide (NaOH) was bought from Nanjing Chemical Reagent Co., Ltd. Green fluorescent protein (GFP) was bought from Solarbio. Ethylenediaminetetraacetic acid disodium salt was bought from Sinopharm Chemical Reagent Co. Ltd. A high voltage power supply was gained from Gamma High Voltage Research. Caco-2 cells, Bovine Serum albumin-FITC, ALP kit (KGT043) and fibroblasts were gained from Keygene Company. Cells were cultured in Eagle's Minimum Essential Medium (EMEM, Gibco, USA) under the condition of 37 °C and 5% CO2. The 8-12-week male mice were from Jinling Hospital. All animals were treated in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health, USA. All animals' experimental protocols and care were reviewed and approved by Animal Investigation Ethics Committee of the Jinling Hospital.
4
Enzymatic Assay of L-DOPA Synthesis
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l ‐DOPA standard and pyruvate sodium were purchased from Aladdin BioChem Technology (Shanghai, China). Catechol, ammonium acetate, sodium sulfite, and ethylenediamine tetraacetic acid disodium salt were purchased from Sinopharm Chemical Reagent (Shanghai, China). The cozyme pyridoxal 5′‐phosphate (PLP) was purchased from Bide Pharmatech (Shanghai, China). Medium components such as tryptone and yeast extract were purchased Oxoid (Basingstoke, England). Antibiotic kanamycin and isopropyl 1‐β‐d ‐thiogalactopyranoside (IPTG) were bought from Sangon Biotech (Shanghai, China). All other chemicals were analytic grade and purchased from commercial sources.
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5
Melamine-Urea-Titanium Oxide Synthesis
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Melamine (C3H6N6), urea [CO(NH2)2], sub-stoichiometric titanium oxide (Ti4O7), sodium hypophosphite (NaH2PO2), sodium sulfate (Na2SO4), isopropanol [IPA, (CH3)2CHOH], ethylenediaminetetraacetic acid disodium salt (C10H14N2Na2O8), sodium hydroxide (NaOH), and sulfuric acid (H2SO4) were provided by Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). 5,5-dimethylpyrroline-N-oxide (DMPO) was bought from Dojindo Co., Ltd. (Shanghai, China). The entire chemical reagents were analytical grade and all solutions were prepared using Milli-Q water (Millipore, 18.2 MΩ cm).
6
Evaluating Antioxidant Capacity of Rosemary Extract
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Ethanol, NaOH, NaCl, potassium ferricyanide, FeCl3, 2-thiobarbituric
acid, trichloroacetic acid (TCA), chloroform (99.5%), mEthanol,
ethylenediamine tetraacetic acid disodium salt, isooctane, acetic acid,
Na2HPO4·12H2O,
NaH2PO4, and 2-propanol were obtained from Sinopharm
Chemical Reagent (Shanghai, China). P-anisidine purity 99% was obtained
from Aladdin Industrial Corporation (Shanghai, China).
1-diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Yuanye Biological
Technology (Shanghai, China). n-Hexane for chromatography (grade 99.5%)
was purchased from Tianjin Guangfu Fine Chemical Research Institute (China).
Borontrifluo-ride-mEthanol (BF3-MeOH purity>50%
(14wt/v%) was obtained from ANPEL Scientific Instruments (Shanghai,
China).
Approximately 350 fresh duck (Anas platyrhucus) eggs weighing
65–75 g, which were retrieved within 3 days of laying. RE was bought from
Henan Yuzhong Biotechnology, China. RE was extracted from dried rosemary
(Rosmarinus oficinalis L.) which is
water-soluble/oil-soluble materials (supplementary Fig. S1). In addition, the
extract contains the main components such as rosmanol, carnosol, and carnosic
acid. Moreover, the studied RE composition is in agreement with Nakatani and Inatani (1981) (link), Zhang et al. (2010) (link), and Rocío Teruel et al. (2015) (link) who
proved that carnosol, rosmanol, and carnosic acid are the main compound in the
RE.
acid, trichloroacetic acid (TCA), chloroform (99.5%), mEthanol,
ethylenediamine tetraacetic acid disodium salt, isooctane, acetic acid,
Na2HPO4·12H2O,
NaH2PO4, and 2-propanol were obtained from Sinopharm
Chemical Reagent (Shanghai, China). P-anisidine purity 99% was obtained
from Aladdin Industrial Corporation (Shanghai, China).
1-diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Yuanye Biological
Technology (Shanghai, China). n-Hexane for chromatography (grade 99.5%)
was purchased from Tianjin Guangfu Fine Chemical Research Institute (China).
Borontrifluo-ride-mEthanol (BF3-MeOH purity>50%
(14wt/v%) was obtained from ANPEL Scientific Instruments (Shanghai,
China).
Approximately 350 fresh duck (Anas platyrhucus) eggs weighing
65–75 g, which were retrieved within 3 days of laying. RE was bought from
Henan Yuzhong Biotechnology, China. RE was extracted from dried rosemary
(Rosmarinus oficinalis L.) which is
water-soluble/oil-soluble materials (supplementary Fig. S1). In addition, the
extract contains the main components such as rosmanol, carnosol, and carnosic
acid. Moreover, the studied RE composition is in agreement with Nakatani and Inatani (1981) (link), Zhang et al. (2010) (link), and Rocío Teruel et al. (2015) (link) who
proved that carnosol, rosmanol, and carnosic acid are the main compound in the
RE.
7
Electrospinning of Polymeric Tubular Grafts
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Poly (ε-caprolactone) (PCL, MW 80,000) and poly (l -lactide-co-caprolactone) (PLCL, MW 80,000) were purchased from Daigang Biomaterial Co. Ltd. (Jinan, China). ethylenediamine tetraacetic acid disodium salt, ethylenediamine, sodium chloroacetate, and cupric sulfate were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). The circular metal rod (Ф 2 mm) was used as a collector for electrospinning tubular grafts. All other chemicals were analytical grade and used without further purification.
8
Antioxidant and Antiglycation Assays
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BSA and gallic acid were provided by the China National Pharmaceutical Group. Glu, FeSO4, 0.01% H2O2, K2S2O8, Folin phenol reagent, potassium ferricyanide, ethylenediaminetetraacetic acid disodium salt (EDTA), and trichloroacetic acid (TCA) were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Lactic acid bacteria (LAB) preserved in a laboratory, arginine (Arg), glyoxal (GO) (40% aqueous solution), Methylglyoxal (40% aqueous solution), aminoguanidine, diammonium salt (ABTS), 1-anilino-8-naphthalene sulfonate (ANS), 1,1-diphenyl-2-picrylhy-drazyl (DPPH), and ortho-phthalaldehyde (OPA) were purchased from Macklin Biochemical Co. (Shanghai, China). In addition, ο-phenylenediamine (ο-PDA), nitroblue tetrazolium (NBT), 2,4-dinitrophenylhydrazine (2,4-DNPH), 1,10-phenanthroline, and 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Aladdin (Shanghai, China). All reagents were of analytical grade.
9
Comprehensive Antioxidant Assays Protocol
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RNA extraction kit was obtained from Beijing TransGen Co., Ltd., China, while qRT-PCR kit and reverse transcription kit were purchased from Beijing Takara Co., Ltd., China.
Chemical reagents (analytical grade), such as dibasic sodium phosphate (Na2HPO4), ferric chloride, potassium ferricyanide, ascorbic acid (AsA), hydrogen peroxide (H2O2), sodium diethyl dithiocarbamate trihydrate, sodium dihydrogen phosphate (NaH2PO4), polyvinyl pyrrolidone (PVP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxylamine hydrochloride, α-naphthyl amine, p-aminobenzenesulfonic acid, sodium nitrite, hydroxylamine, trichloroacetic acid, thibabituric acid, dithiothreitol, nitro blue tetrazolium, riboflavin, ethylenediaminetetraacetic acid disodium salt (EDTA-Na2), bovine serum albumin, catechin, gallic acid, methionine, dithio nitrobenzoic acid (DTNB), glutathione (GSH), methanol, were obtained from Shanghai Sinopharm Co. Ltd., China.
Chemical reagents (analytical grade), such as dibasic sodium phosphate (Na2HPO4), ferric chloride, potassium ferricyanide, ascorbic acid (AsA), hydrogen peroxide (H2O2), sodium diethyl dithiocarbamate trihydrate, sodium dihydrogen phosphate (NaH2PO4), polyvinyl pyrrolidone (PVP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxylamine hydrochloride, α-naphthyl amine, p-aminobenzenesulfonic acid, sodium nitrite, hydroxylamine, trichloroacetic acid, thibabituric acid, dithiothreitol, nitro blue tetrazolium, riboflavin, ethylenediaminetetraacetic acid disodium salt (EDTA-Na2), bovine serum albumin, catechin, gallic acid, methionine, dithio nitrobenzoic acid (DTNB), glutathione (GSH), methanol, were obtained from Shanghai Sinopharm Co. Ltd., China.
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