The morphology of the Ag nanoparticles was observed with a transmission electron microscopy (TEM) (HT770) operating at 80 kV and a scanning electron microscope (SEM) (Hitachi SU-8010) operating at 5.0 kV. The microstructure of the electrodeposited Ag nanoparticles was determined by an X-ray diffraction spectrometer (XRD) (D/Max 2550 pc). The formation efficiency of the Ag nanoparticles was estimated using a thermal gravimetric analyzer (DSCQ1000) through annealing the colloidal solution under the protection of nitrogen. All of the photographs were recorded by a digital camera (Canon EOS750D). SERS measurements were performed on a confocal Raman microscopic system (LabRAM HR Evolution, France). The excitation wavelength was 633 nm generated by an Nd: yttrium-aluminum-garnet laser operating at a power of about 0.25 mW. The accumulation time for the SERS signals was 10 s. Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) measurements were operated on a Nicolet 5700 FTIR spectrometer and an Axis Supra XPS spectrometer, respectively. The absorption spectra were obtained using an ultraviolet-visible-near infrared spectrophotometer (PerkinElmer Lambda 950).
Ht770
Manufactured by Hitachi
Sourced in Japan
The HT770 is a laboratory equipment product manufactured by Hitachi. It is designed to perform specific technical functions within a laboratory setting. The core function of the HT770 is to provide precise and accurate measurements or analysis, but a detailed description without interpretation or extrapolation cannot be provided.
Automatically generated - may contain errors
Lab products found in correlation
Su8010, by Hitachi (2 mentions)
S 4800, by Hitachi (2 mentions)
Dxr raman microscope, by Thermo Fisher Scientific (2 mentions)
Eos 750d, by Canon (1 mentions)
Lambda 950, by PerkinElmer (1 mentions)
Pmx 110, by Particle Metrix (1 mentions)
Type 70 ti rotor, by Beckman Coulter (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Bca protein assay kit, by Aspen (1 mentions)
Fluoromax 4 spectrofluorometer, by Horiba (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
15 s copper grid 400 mesh, by ProSciTech (1 mentions)
Hexafluoro 2 propanol treated aβ42, by AnaSpec (1 mentions)
Zetapals analyzer, by Brookhaven Instruments (1 mentions)
Spm 9700 instrument, by Shimadzu (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Rf 5301pc, by Shimadzu (1 mentions)
U 3900, by Hitachi (1 mentions)
Jem 2100, by Hitachi (1 mentions)
Glutaraldehyde, by Wuhan Servicebio Technology (1 mentions)
25 protocols using ht770
1
Comprehensive Characterization of Ag Nanoparticles
2
Isolation and Characterization of Exosomes
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RAW264.7 cells were cultured in DMEM containing EXO-free FBS (ultracentrifugation at 120,000 ×g for 12 h) before LPS stimulation. RAW264.7 cells were cultured to 80% confluence, and then the control group was not given any treatment. The LPS group was stimulated with 1 µg/mL LPS (#L2880; Sigma, St Loui, MO, USA) for 24 h, and the supernatant was collected and subjected to a series of gradient centrifugation to extract the EXOs.
For the extraction of renal EXOs, 20 mg of renal cortex was digested with collagenase at 37 °C for 120 min. The samples were then subjected to EXOs extraction. The gradient centrifugation included 300 ×g for 10 min and 2000 ×g for 10 min, followed by 10000×g for 30 min. The supernatants were then centrifuged at 120000 ×g for 70 min. The pellets were washed once with PBS, centrifuged again at 120000×g for 70 min and resuspended in PBS (Type 70 Ti rotor; Beckman Coulter Optima, USA) .
The morphology of RAW264.7-EXO was examined using a transmission electron microscope (Hitachi HT770, Tokyo, Japan). Nanoparticle tracking analysis (NTA) was performed by Zetaview, PMX 110 (Particle Metrix, Meerbusch, Germany), and the protein levels was quantified using the BCA Protein Assay Kit (Aspen, Wuhan, China) following the manufacturer’s instructions.
For the extraction of renal EXOs, 20 mg of renal cortex was digested with collagenase at 37 °C for 120 min. The samples were then subjected to EXOs extraction. The gradient centrifugation included 300 ×g for 10 min and 2000 ×g for 10 min, followed by 10000×g for 30 min. The supernatants were then centrifuged at 120000 ×g for 70 min. The pellets were washed once with PBS, centrifuged again at 120000×g for 70 min and resuspended in PBS (Type 70 Ti rotor; Beckman Coulter Optima, USA) .
The morphology of RAW264.7-EXO was examined using a transmission electron microscope (Hitachi HT770, Tokyo, Japan). Nanoparticle tracking analysis (NTA) was performed by Zetaview, PMX 110 (Particle Metrix, Meerbusch, Germany), and the protein levels was quantified using the BCA Protein Assay Kit (Aspen, Wuhan, China) following the manufacturer’s instructions.
3
Comprehensive Characterization of Graphene Quantum Dots
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The optical properties of GQD-1 and GQD-2 were analyzed using a Biochrom
UV–vis absorption spectrophotometer. Photoluminescence (PL)
characteristics of GQD-1 and GQD-2 were analyzed using a FluoroMax-4
Spectrofluorometer—Horiba, the fluorescence spectrophotometer.
GQDs illustrate tunable PL through the manipulation of edge functionality
under distinct preparation conditions. In the current study, the FTIR
instrument employed was 760 Nicolet, and it helped in the detection
of organic as well as inorganic groups present in the GQD samples,
in accordance with their particular IR frequency. The morphologic
characteristics of GQD samples were analyzed using transmission electron
microscopy (HT 770, Hitachi, Japan). The instrument employed for Raman
spectroscopy was a Thermo Fisher Scientific DXR Raman microscope having
a wavelength of 532 nm, 40 times scanning, and a laser power of 0.1–10
mW using 50× microscope objectives. Furthermore, the presence
of GQDs was clearly confirmed by the peaks noted from the Fourier
transform infrared spectroscopy (FTIR) analysis. The FTIR instrument
employed for the GQD analysis, in this study, was a 760 Nicolet FTIR
model. NMR analysis for the two samples for 1H spectra
and 13C was carried out using a JOEL NMR 600 MHz.
UV–vis absorption spectrophotometer. Photoluminescence (PL)
characteristics of GQD-1 and GQD-2 were analyzed using a FluoroMax-4
Spectrofluorometer—Horiba, the fluorescence spectrophotometer.
GQDs illustrate tunable PL through the manipulation of edge functionality
under distinct preparation conditions. In the current study, the FTIR
instrument employed was 760 Nicolet, and it helped in the detection
of organic as well as inorganic groups present in the GQD samples,
in accordance with their particular IR frequency. The morphologic
characteristics of GQD samples were analyzed using transmission electron
microscopy (HT 770, Hitachi, Japan). The instrument employed for Raman
spectroscopy was a Thermo Fisher Scientific DXR Raman microscope having
a wavelength of 532 nm, 40 times scanning, and a laser power of 0.1–10
mW using 50× microscope objectives. Furthermore, the presence
of GQDs was clearly confirmed by the peaks noted from the Fourier
transform infrared spectroscopy (FTIR) analysis. The FTIR instrument
employed for the GQD analysis, in this study, was a 760 Nicolet FTIR
model. NMR analysis for the two samples for 1H spectra
and 13C was carried out using a JOEL NMR 600 MHz.
4
Ultrastructural Analysis of Endometrial Cells
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Two-millimeter-thick uterine tissues were placed in 3% glutaraldehyde and 1% osmium tetroxide for at least 24 h at 4°C as previously reported. The tissues were then dehydrated, embedded and cut into 70-nm thick sections. Finally, the samples were dried with a HitachiES-2030 dryer, sprayed with gold with a HitachiE-1010 injector, and observed with a Hitachi HT770 transmission electron microscope. Changes in organelles in endometrial cells were observed at different magnifications, and additional morphological features of the epithelia were observed independently by two investigators.
5
Characterizing Micelle Size and Morphology
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The size of the micelles was determined in cuvettes using a Zetasizer Nano ZS90 (Malvern) instrument. Micelle morphologies were analyzed by transmission electron microscopy (TEM) using a Hitachi HT770 electron microscope operating at 100 kV. To that end, copper TEM grids were covered with a layer of carbon to deposit the sample. The micelle solution was then dropped on the grid and dried for about 20 min. The sample was analyzed on the microscope unstained or negatively stained. For negative staining, a drop of phosphotungstic acid (2 %) was added to the sample to stain for 1–3 min. Excess deposits were absorbed by filter paper and the sample was air-dried.
6
Aβ42 Amyloid Fibril Formation Visualized by TEM
7
Ultrastructural Changes in Cd-Stressed Microalgae
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To further understand the response of S. obliquus to Cd2+ stress, the morphology of microalgal cells was observed by scanning electron microscopy (SEM, Hitachi S-4800, Hitachi, Ltd., Tokyo, Japan) and transmission electron microscopy (TEM, Hitachi HT-770, Ltd. Tokyo, Japan). Microalgae cells were fixed with 2.5% glutaraldehyde at 4 °C for 24 h, then washed with phosphate buffer (0.05 M, pH 7.0), dehydrated with 30%, 50%, 70%, 90%, and 100% ethanol, and finally sputtered with gold before SEM analysis. The morphology of the cells was observed by SEM.
For TEM analysis, cell samples from control and Cd-treated cultures respectively were centrifuged, fixed, dehydrated, sectioned, and stained. The ultrastructural changes of S. obliquus cells were observed by TEM.
For TEM analysis, cell samples from control and Cd-treated cultures respectively were centrifuged, fixed, dehydrated, sectioned, and stained. The ultrastructural changes of S. obliquus cells were observed by TEM.
8
Evaluating Nanoparticle Encapsulation Efficiency
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The encapsulation efficiency (EE, %) of different nanoparticles was measured by ultra-filtration method using Amicon Ultra-4 centrifugal filter devices. In brief, FAM-conjugated siRNA was used to prepare different LRs and formulations were added into the device with a centrifugal speed of 4,000 rpm for 10 minutes. Free siRNA was collected and measured according to a standard curve (data not shown). The EE (%) of different formulations was calculated according to the formula:
where Ct and Cu represented the amount of total siRNA and unloaded siRNA, respectively.
Particle size distribution and ζ-potential of different formulations were measured using dynamic light scattering instrument (Zetasizer Nano ZS). The morphology of hybrid nanoparticles was observed using transmission electron microscopy (TEM, HT770; Hitachi, Tokyo, Japan) and atomic force microscopy (AFM), respectively.
In order to evaluate the pH-sensitivity of PHD/LR, it was diluted with PBS at different pH (pH 7.4 and 5.0) and at different time points, and ζ-potential of different samples were measured.
where Ct and Cu represented the amount of total siRNA and unloaded siRNA, respectively.
Particle size distribution and ζ-potential of different formulations were measured using dynamic light scattering instrument (Zetasizer Nano ZS). The morphology of hybrid nanoparticles was observed using transmission electron microscopy (TEM, HT770; Hitachi, Tokyo, Japan) and atomic force microscopy (AFM), respectively.
In order to evaluate the pH-sensitivity of PHD/LR, it was diluted with PBS at different pH (pH 7.4 and 5.0) and at different time points, and ζ-potential of different samples were measured.
9
Nanocellulose Characterization by TEM, XRD, and Titration
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Transmission
electron
microscopy (TEM) at 80 kV (Hitachi HT770) was used to study the morphology
of the various nanocellulose types. Samples were prepared by dropping
a dilute suspension of the nanocellulose onto a glow-discharged copper
TEM grid, which was allowed to dry before being stained with uranyl
acetate (2% w/v) for 10 min. The crystallinity index (CI) for each
nanocellulose type was determined by X-ray diffraction (XRD) (Bruker
D8 Advance XRD). Graphite-filtered Cu Kα radiation was generated
at 40 kV and 40 mA. All samples were scanned over a range of 2θ
= 10–90° at a scan rate of 1.2 s/step. The acidic group
content in the different nanocellulose types was quantified by conductometric
titration according to the Scandinavian pulp, paper, and board standard
method (SCAN-CM 65:0213 ) under standard
lab conditions without variation.
electron
microscopy (TEM) at 80 kV (Hitachi HT770) was used to study the morphology
of the various nanocellulose types. Samples were prepared by dropping
a dilute suspension of the nanocellulose onto a glow-discharged copper
TEM grid, which was allowed to dry before being stained with uranyl
acetate (2% w/v) for 10 min. The crystallinity index (CI) for each
nanocellulose type was determined by X-ray diffraction (XRD) (Bruker
D8 Advance XRD). Graphite-filtered Cu Kα radiation was generated
at 40 kV and 40 mA. All samples were scanned over a range of 2θ
= 10–90° at a scan rate of 1.2 s/step. The acidic group
content in the different nanocellulose types was quantified by conductometric
titration according to the Scandinavian pulp, paper, and board standard
method (SCAN-CM 65:0213 ) under standard
lab conditions without variation.
10
Characterizing DNA Nanostructures
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To confirm the successful synthesis of TDNs and Apt‐TDNs, molecular weights of DNA nanostructures were evaluated using 8% polyacrylamide gel electrophoresis. Transmission electronic microscopy (TEM; HT770, Hitachi, Tokyo, Japan) was performed to observe the morphology of s‐TDN. The size of TDN was evaluated by dynamic light scattering (DLS; Malvern Instruments Ltd, Malvern, UK).
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