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G1780

Manufactured by Promega
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Sourced in United States

The G1780 is a Promega product that functions as a protein purification system. It is designed to facilitate the efficient isolation and separation of target proteins from complex mixtures.

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Lab products found in correlation

Cytotox 96, by Promega (3 mentions) Trypan blue, by Thermo Fisher Scientific (3 mentions) Countess cell counting chamber slide, by Thermo Fisher Scientific (3 mentions) Countess, by Thermo Fisher Scientific (3 mentions) μquant, by Agilent Technologies (2 mentions) Quant, by Agilent Technologies (2 mentions) Prestoblue cell viability reagent, by Thermo Fisher Scientific (1 mentions) Spectramax id3, by Molecular Devices (1 mentions) Ab155902, by Abcam (1 mentions) Elisa, by R&D Systems (1 mentions) Sircol soluble collagen assay, by Biocolor (1 mentions) Cytotox 96 non radioactive cytotoxicity assay, by Promega (1 mentions) Recombinant human pd 1 fc protein, by Sino Biological (1 mentions) Anti pd l1 antibody, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Digitonin, by Merck Group (1 mentions) Recombinant murine m csf, by Genentech (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Sytox green nucleic acid stain, by Thermo Fisher Scientific (1 mentions)

42 protocols using g1780

1

Quantifying Cell Cytotoxicity via LDH Assay

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50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly, 50μl of the CytoTox 96® Reagent was added to each sample aliquot. The plate was covered with foil to protect it from light, and incubated for 30 minutes at room temperature on shaker. 50μl of Stop Solution was added to each well of the 96-well plate, and the absorbance recorded at 490nm with the plate reader. Each experiment was repeated at least 3 times with triplicate wells each time.
Jiang L., Lin W., Zhang C., Ash P.E., Verma M., Kwan J., van Vliet E., Yang Z., Cruz A.L., Boudeau S., Maziuk B.F., Lei S., Song J., Alvarez V.E., Hovde S., Abisambra J.F., Kuo M.H., Kanaan N., Murray M.E., Crary J.F., Zhao J., Cheng J.X., Petrucelli L., Li H., Emili A, & Wolozin B. (2021). Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy. Molecular cell, 81(20), 4209-4227.e12.
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2

Cytotoxicity Assay for Lactate Dehydrogenase

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Levels of lactate dehydrogenase released by cells were determined using the CytoTox 96 Non-Radioactive Cytotoxicity Assay according to the manufacturer’s instructions (G1780, Promega).
Fox D., Mathur A., Xue Y., Liu Y., Tan W.H., Feng S., Pandey A., Ngo C., Hayward J.A., Atmosukarto I.I., Price J.D., Johnson M.D., Jessberger N., Robertson A.A., Burgio G., Tscharke D.C., Fox E.M., Leyton D.L., Kaakoush N.O., Märtlbauer E., Leppla S.H, & Man S.M. (2020). Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome. Nature Communications, 11, 760.
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3

Cytotoxicity Assay of YYB-103

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Target cells (10,000 cells/well) and effector cells were mixed according to the indicated effector-to-target (E:T) cell ratio and co-cultured in a V-bottom 96-well plate at 37°C for 18 h in a CO2 incubator. The cytotoxicity of YYB-103 was determined by a CytoTox96 non-radioactive cytotoxicity assay measuring the release of lactate dehydrogenase (Promega, G1780, Madison, WI, USA) according to the manufacturer’s instructions.
Kim K., Gwak H.S., Han N., Hong E.K., Choi B.K., Lee S., Choi S., Park J.H., Seok J.H., Jeon Y., Cho H., Lee S.J., Lee Y., Nam K.T, & Song S.W. (2021). Chimeric Antigen Receptor T Cells With Modified Interleukin-13 Preferentially Recognize IL13Rα2 and Suppress Malignant Glioma: A Preclinical Study. Frontiers in Immunology, 12, 715000.
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4

Quantifying Cell Viability in Microfluidic Chips

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Urea nitrogen (SB-0580–250, Stanbio, Boerne, TX) and lactate dehydrogenase (LDH; G1780, Promega, Madison, WI) levels were measured from 2D and 3D effluent/media samples following manufacture’s protocols. Cell viability (A13261, PrestoBlue Cell Viability Reagent, Invitrogen) was measured on day 10 of culture. Briefly, a 1x solution of PrestoBlue reagent in HMM was added to syringes and perfused through chips at a rate of 50 μL/hr for 2 hours at 37°C. The perfusion rate in the experiments with PrestoBlue was temporarily increased to enable collection of the sufficient volume of the effluent as we found that the reagents are unstable in the incubator over periods of longer perfusion (data not shown). In 96-well plates, 100 μL of 1X solution of PrestoBlue reagent in HMM was added to each well for 2 hours at 37°C. After incubation, media was collected from chips and wells, and fluorescence was measured at 560/590 nm on a plate reader (SpectraMax iD3, Molecular Devices). Values for all assays were normalized to vehicle (0.1% DMSO).
Sakolish C., Reese C.E., Luo Y.S., Valdiviezo A., Schurdak M.E., Gough A., Taylor D.L., Chiu W.A., Vernetti L.A, & Rusyn I. (2020). Analysis of Reproducibility and Robustness of a Human Microfluidic Four-Cell Liver Acinus MicroPhysiology System (LAMPS). Toxicology, 448, 152651.
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5

Cytotoxicity and Inflammation Assays

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Relative cytotoxicity was assessed by lactate dehydrogenase assay (G1780; Promega) and expressed as a value relative to control. The production of inflammatory cytokines (IL‐8, IL‐6, TNF‐α and IL‐18) by the cells was quantified in the supernatant by ELISA (R&D Systems). Cell proliferation and collagen production were assessed by WST‐1 assay (ab155902; Abcam) and Sircol Soluble Collagen Assay (S5000; Biocolor), respectively. All experiments were repeated six times (fresh cell and coal preparations on different days) to allow statistical comparisons to be made between samples.
Song Y., Southam K., Beamish B.B, & Zosky G.R. (2022). Effects of chemical composition on the lung cell response to coal particles: Implications for coal workers' pneumoconiosis. Respirology (Carlton, Vic.), 27(6), 447-454.
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6

Quantifying Cellular Cytotoxicity via LDH

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Levels of lactate dehydrogenase released by cells were determined using the CytoTox 96 Non‐Radioactive Cytotoxicity Assay according to the manufacturer's instructions (G1780, Promega).
Enosi Tuipulotu D., Feng S., Pandey A., Zhao A., Ngo C., Mathur A., Lee J., Shen C., Fox D., Xue Y., Kay C., Kirkby M., Lo Pilato J., Kaakoush N.O., Webb D., Rug M., Robertson A.A., Tessema M.B., Pang S., Degrandi D., Pfeffer K., Augustyniak D., Blumenthal A., Miosge L.A., Brüstle A., Yamamoto M., Reading P.C., Burgio G, & Man S.M. (2023). Immunity against Moraxella catarrhalis requires guanylate‐binding proteins and caspase‐11‐NLRP3 inflammasomes. The EMBO Journal, 42(6), e112558.
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7

Quantifying Cellular Cytotoxicity by LDH

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Levels of lactate dehydrogenase released by cells were determined using the CytoTox 96 Non-Radioactive Cytotoxicity Assay according to the manufacturer’s instructions (G1780, Promega). Cell culture supernatants were collected for ELISA.
Ming Man S., Karki R., Briard B., Burton A., Gingras S., Pelletier S, & Kanneganti T.D. (2017). Differential roles of caspase-1 and caspase-11 in infection and inflammation. Scientific Reports, 7, 45126.
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8

Quantifying Cellular Cytotoxicity via LDH

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Levels of lactate dehydrogenase released by cells were determined using the CytoTox 96 Non‐Radioactive Cytotoxicity Assay according to the manufacturer's instructions (G1780, Promega).
Mathur A., Kay C., Xue Y., Pandey A., Lee J., Jing W., Enosi Tuipulotu D., Lo Pilato J., Feng S., Ngo C., Zhao A., Shen C., Rug M., Miosge L.A., Atmosukarto I.I., Price J.D., Ali S.A., Gardiner E.E., Robertson A.A., Awad M.M., Lyras D., Kaakoush N.O, & Man S.M. (2023). Clostridium perfringens virulence factors are nonredundant activators of the NLRP3 inflammasome. EMBO Reports, 24(6), e54600.
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9

Lactate Dehydrogenase Cytotoxicity Assay

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Secreted levels of lactate dehydrogenase from cell supernatants were determined using the CytoTox 96 Non-Radioactive Cytotoxicity Assay according to the manufacturer’s instructions (G1780; Promega).
Malireddi R.K., Gurung P., Mavuluri J., Dasari T.K., Klco J.M., Chi H, & Kanneganti T.D. (2018). TAK1 restricts spontaneous NLRP3 activation and cell death to control myeloid proliferation. The Journal of Experimental Medicine, 215(4), 1023-1034.
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10

Evaluating Salmonella Cytotoxicity and Inhibition

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Cytotoxicity was assessed using a lactate dehydrogenase (LDH) release assay (Promega, G1780). The percentage of LDH released was calculated using the following formula: percentage of release = (experimental LDH release—spontaneous LDH release)/(maximal LDH release—spontaneous LDH release) × 100%. To evaluate the effect of the inhibitors in Salmonella, a kinetic assay was conducted. Inhibitor concentrations were maintained, as previously shown. Assays were performed over the 8 h; for each hour, the optic density was evaluated. At the end of the lapse time, CFU formation was evaluated through plating on LB agar.
Luis L.B., Ana G.T., Carlos G.E., Abraham G.G., Iris E.G., Martha M.L, & Vianney O.N. (2022). Salmonella Promotes Its Own Survival in B Cells by Inhibiting Autophagy. Cells, 11(13), 2061.
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