Metamorph software

Manufactured by Leica

Sourced in Germany

MetaMorph is a comprehensive software suite for controlling microscope hardware and analyzing digital images. It provides advanced capabilities for high-throughput, multiwavelength, and time-lapse image acquisition. MetaMorph also offers robust tools for quantitative image analysis and data visualization.

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Lab products found in correlation

Dmi6000 inverted microscope, by Leica (3 mentions) Dfc350fxr2 ccd camera, by Leica (3 mentions) Nanozoomer 2.0 ht scanner, by Hamamatsu Photonics (2 mentions) Inverted confocal sp5, by Leica (2 mentions) Mm af, by Leica (2 mentions) Hc pl apo 10x 0.40 dry objective, by Leica (2 mentions) Application suite x, by Leica (1 mentions) Confocal microscope, by Leica (1 mentions) Confocal microscopy, by Leica (1 mentions) Dapi fluoromount g solution, by Southern Biotech (1 mentions) Ndp view2 viewing software, by Hamamatsu Photonics (1 mentions) Bright field microscope, by Leica (1 mentions) Dmi6000b, by Leica (1 mentions) Axioscan, by Zeiss (1 mentions) Las af software, by Leica (1 mentions) Zen software, by Zeiss (1 mentions) Ndp view2, by Hamamatsu Photonics (1 mentions) Fluorescence microscope, by Olympus (1 mentions) Matrigel, by BD (1 mentions) Lsm 710 microscope, by Zeiss (1 mentions)

Close spelling variants of this product

Metamorph Imaging Software Software MMAF Version 1.4.0 MetaMorph MetaMorph

13 protocols using metamorph software

Quantifying Neutrophil Extracellular Traps

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NET formation was quantified by measuring histone area^{14 (link)}. Neutrophils were seeded onto coverslips and processed as described above for determination of NET structure. Images were collected using a Leica confocal microscope and processed using Leica Application Suite X and MetaMorph software. Briefly, NETs were visualized in at least five random fields (×40 magnification), signal intensity of histone per field was individually measured and the pixels count for each image was converted into area (μm²) using a calibration unit (0.8333). Mean histone area (μm²) was determined from the five independent fields.

Sung P.S., Huang T.F, & Hsieh S.L. (2019). Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nature Communications, 10, 2402.

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Quantification of FAK, p53, and p21 in HUVECs

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HUVECs were seeded onto glass coverslips for 24 h, followed by treatment with cordycepin for another 24 h. Cells were fixed with 2% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 10% FBS, followed by incubation with specific antibodies for FAK, p53, and p21. Then, the cells were washed with 0.02% Triton X-100, labeled with secondary antibodies, and mounted in DAPI Fluoromount-G solution (Southern Biotech, Birmingham, AL, USA). The expression levels and subcellular localization of FAK, p53, and p21 were examined by confocal microscopy (Leica). Number and length of focal adhesions, FAK, p53, and p21 expression levels in ten random fields (34–53 cells) were quantified using MetaMorph software (Version 7.10.1.161, Leica, Wetzlar, Germany).

Lin Y.T., Liang S.M., Wu Y.J., Wu Y.J., Lu Y.J., Jan Y.J., Ko B.S., Chuang Y.J., Shyue S.K., Kuo C.C, & Liou J.Y. (2019). Cordycepin Suppresses Endothelial Cell Proliferation, Migration, Angiogenesis, and Tumor Growth by Regulating Focal Adhesion Kinase and p53. Cancers, 11(2), 168.

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Quantifying Pericyte and Fibroblast Populations

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Histological slides were scanned using Nanozoomer 2.0 HT scanner with a 40× objective and visualized with NDP.view2 viewing software (Hamamatsu). Fluorescent images were acquired on a brightfield microscope (Leica) using Metamorph software, inverted Confocal SP5 (Leica) using the Leica LAS AF software, or an Andor Dragonfly Spinning Disk Confocal microscope employing Imaris software. Quantification of PC and vimentin^positive fibroblasts was done on at least five random fields (about 1 mm² each) per sample using Imaris and ImageJ software. Images were processed with ImageJ. Images were assembled and adjusted with Adobe Photoshop/ Illustrator. Counting of PC was performed using z‐stack acquisition. Co‐staining with cell markers was confirmed on distinct layers. In analyses of human biopsies, PC numbers were calculated either per mm² or per Vimentin‐stained area using an arbitrary unit (AU corresponding to 10,000 square pixel) of vimentin^positive cells.

Paul C., Tang R., Longobardi C., Lattanzio R., Eguether T., Turali H., Bremond J., Maurizy C., Gabola M., Poupeau S., Turtoi A., Denicolai E., Cufaro M.C., Svrcek M., Seksik P., Castronovo V., Delvenne P., de Laurenzi V., Da Costa Q., Bertucci F., Lemmers B., Pieragostino D., Mamessier E., Janke C., Pinet V, & Hahne M. (2022). Loss of primary cilia promotes inflammation and carcinogenesis. EMBO Reports, 23(12), e55687.

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Quantification of Tissue Composition in Defect Region

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Evaluation of the defect region was performed using a light microscope (Leica DMI6000 B, Leica Microsystems, Wetzlar, Germany) and image analysis software (Leica MetaMorph^® software). The defect region was analyzed for bone, cartilage and fibrous tissue contents. The sections were digitalized using 50× magnification. The digitalized images were then used for the analysis. Using the MetaMorph^® Software and a customized plug-in, the area of the former defect region was outlined as well as the outlines of bone and cartilage areas. The area was automatically calculated by the program and the soft tissue area was determined by subtracting bone and cartilage from the defect area. The relative proportions in relation to the defect area were calculated using Microsoft Excel.

Rapp A.E., Bindl R., Erbacher A., Kruchen A., Rojewski M., Schrezenmeier H., Müller I, & Ignatius A. (2018). Autologous Mesenchymal Stroma Cells Are Superior to Allogeneic Ones in Bone Defect Regeneration. International Journal of Molecular Sciences, 19(9), 2526.

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Fibroblast Migration Assay

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Fibroblasts from at least 3 biologically unique samples were seeded at low density, in a 24-well-plate, starved 24 h, and underwent various treatments. The plate was set on a motorized stage in a 5 % CO₂ and 37 °C environment. Random regions were marked, and Leica Live Imaging Software snapped a photo of these regions every 5 min for 20 h. LIF files were exported and analyzed in Leica MetaMorph software version 1.5.0. The average total distance traveled of at least 6 random cells per group were tracked and recorded.

MacKenzie B., Korfei M., Henneke I., Sibinska Z., Tian X., Hezel S., Dilai S., Wasnick R., Schneider B., Wilhelm J., El Agha E., Klepetko W., Seeger W., Schermuly R., Günther A, & Bellusci S. (2015). Increased FGF1-FGFRc expression in idiopathic pulmonary fibrosis. Respiratory Research, 16(1), 83.

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Multimodal Imaging of Biological Samples

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Histological slides were scanned using the Nanozoomer 2.0 HT scanner with a ×40 objective, and visualized with the NDP.view2 software (Hamamatsu). Fluorescent images were acquired with Axioscan using ZEN software (Zeiss), brightfield Axioimager Z1 (Zeiss) using the Metamorph software or on the inverted Confocal SP5 (Leica) using the Leica LAS AF software. Images were processed with Adobe Photoshop CS6.

Maurizy C., Abeza C., Lemmers B., Gabola M., Longobardi C., Pinet V., Ferrand M., Paul C., Bremond J., Langa F., Gerbe F., Jay P., Verheggen C., Tinari N., Helmlinger D., Lattanzio R., Bertrand E., Hahne M, & Pradet-Balade B. (2021). The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium. Nature Communications, 12, 4810.

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Angiogenesis Assay with HUVEC

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HUVEC (2 × 10⁴ cells/mL) were seeded in a 48-well plate pre-coated with Matrigel (BD Biosciences, San Jose, CA, USA). After Huh7 cell-conditioned medium was added to a final volume of 20%, the cells were cultured for 16 hours, stained with calcium-AM (Invitrogen), and visualized under a fluorescence microscope (Olympus). Total tube lengths were measured by MetaMorph software (Leica)

Tsai C.F., Hsieh T.H., Lee J.N., Hsu C.Y., Wang Y.C., Lai F.J., Kuo K.K., Wu H.L., Tsai E.M, & Kuo P.L. (2014). Benzyl butyl phthalate induces migration, invasion, and angiogenesis of Huh7 hepatocellular carcinoma cells through nongenomic AhR/G-protein signaling. BMC Cancer, 14, 556.

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Live-cell Imaging and FRET Analysis

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Cells were plated on fibronectin-coated coverglass (2-well Lab-Tek with 10 μg/ml fibronectin) 16 h before imaging and then transferred into complete medium without phenol red. Live cell imaging was performed in a CO₂ and thermo-regulated chamber using a spinning disk microscope equipped with a 63X oil inverted objective. Time-lapse movies were taken at 1 min (cell dynamics) or 10 mins (cell migration) time intervals. Imaging data have been collected by Leica Metamorph software (version: Metamorph 7.8.13.0).
FRET images of live cells were acquired with a Zeiss LSM710 microscope. A 458 nm laser was used to excite the sample. CFP and YFP emission signals were collected through channel I (470–510 nm) and channel II (525–600 nm), respectively. To capture single, high-resolution, stationary images, a 40X/1.3 oil inverted objective was used. CFP and YFP images were acquired simultaneously for most of the experiments. Sequential acquisition of CFP and YFP channels in alternative orders were tested and gave the same result as simultaneous acquisition. Imaging data have been collected by Zeiss Zen software (version: Zen 2007 light edition).

Chadelle L., Liu J., Choesmel-Cadamuro V., Karginov A.V., Froment C., Burlet-Schiltz O., Gandarillas S., Barreira Y., Segura C., Van Den Berghe L., Czaplicki G., Van Acker N., Dalenc F., Franchet C., Hahn K.M., Wang X, & Belguise K. (2021). PKCθ-mediated serine/threonine phosphorylations of FAK govern adhesion and protrusion dynamics within the lamellipodia of migrating breast cancer cells. Cancer letters, 526, 112-130.

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Time-Lapse Microscopy of mdx and WT Myoblasts

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15,000 myoblasts (mdx or w/t) were seeded into a 24-well cell culture plate and grown for 48 h in the culture medium. Time-lapse movies were generated by multiple well areas per each cell type being photographed every 15 min for 5 h 45 min using the inverted DMI6000 microscope (Leica Microsystems GmbH) equipped with an environmental chamber (PeCon GmbH) and a DFC350FXR2 CCD camera (Leica Microsystems GmbH). Both the bright field and the DIC Nomarski contrast using HC PL APO 10×/0.40 dry objective (Leica Microsystems GmbH) were captured. Acquired time-lapse movies were exported to TIFF format and aligned to compensate for possible drifts using an ImageJ plugin. Subsequently, at least 30 cells per each experimental condition were tracked semi-automatically in the time-lapse movies using the Track Objects plug-in in Leica MM AF powered by MetaMorph^® software (Leica Microsystems GmbH). Cells dividing or colliding with other cells were excluded from the analysis. The statistical significance was evaluated as described below.

Róg J., Oksiejuk A., Górecki D.C, & Zabłocki K. (2023). Primary mouse myoblast metabotropic purinoceptor profiles and calcium signalling differ with their muscle origin and are altered in mdx dystrophinopathy. Scientific Reports, 13, 9333.

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Fluorescent Staining of Cells for Microscopy

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To prepare cells for fluorescent staining, cells were cultured in one‐ or two‐well sterile tissue culture‐treated chamber slides (Lab‐Tek II Chamber Slides; Nunc, Rochester, NY, USA) or on sterile poly‐l‐lysine (1 mg·mL⁻¹; Sigma‐Aldrich) coated glass coverslips for 48–72 h. Following treatment with the drugs described above, cells were fixed to preserve TNTs with 16% w/v paraformaldehyde. Fluorescent staining for actin was performed with phalloidin rhodamine (4 units·mL⁻¹) in culture medium with 0.05% Triton X. Mitochondria were stained using MitoTracker Green (100 nm) in media. Images were captured using a Leica SP5 Confocal Microscope with metamorph software (Leica Microsystems, Bannockburn, IL, USA).

Kato K., Nguyen K.T., Decker C.W., Silkwood K.H., Eck S.M., Hernandez J.B., Garcia J, & Han D. (2021). Tunneling nanotube formation promotes survival against 5‐fluorouracil in MCF‐7 breast cancer cells. FEBS Open Bio, 12(1), 203-210.

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