Maxwell 16 tissue dna purification kit

Manufactured by Promega

Sourced in United States, Italy

The Maxwell 16 Tissue DNA Purification Kit is a lab equipment product designed for the automated purification of genomic DNA from a variety of tissue samples. It uses magnetic bead-based technology to isolate and purify DNA, providing a reliable and efficient method for sample preparation.

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Lab products found in correlation

Maxwell 16 instrument, by Promega (10 mentions) Nanodrop 2000, by Thermo Fisher Scientific (10 mentions) Maxwell 16 ffpe plus lev dna purification kit, by Promega (5 mentions) Fluostar omega microplate reader, by BMG Labtech (4 mentions) Sample purification beads, by Illumina (4 mentions) Hiseq system, by Illumina (4 mentions) Truseq nano dna sample prep kit, by Illumina (4 mentions) Maxwell 16 ffpe plus lev dna kit, by Promega (3 mentions) Qiacube, by Qiagen (3 mentions) Mirneasy kit, by Qiagen (3 mentions) S220 sonicator, by Covaris (3 mentions) Qubit dsdna br assay kit, by Thermo Fisher Scientific (3 mentions) Qubit 3.0 fluorometer, by Thermo Fisher Scientific (3 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (3 mentions) Maxwell 16 blood dna purification kit, by Promega (2 mentions) Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions) Maxwell 16 extractor, by Promega (2 mentions) Iscript advanced cdna synthesis kit, by Bio-Rad (2 mentions) Quantasoft software, by Bio-Rad (2 mentions) Maxwell rsc blood dna kit, by Promega (2 mentions)

Close spelling variants of this product

Maxwell 16 (79 citations) Maxwell kits (2 citations) Maxwell 16 Tissue and Blood DNA purification kits (2 citations)

91 protocols using maxwell 16 tissue dna purification kit

Tomato Genome Sequencing with PacBio

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Total genomic DNA was extracted from young leaves of the six tomato lines (‘CMS[MSA1]’, ‘CMS[O]’, ‘CMS[P]’, ‘Sekai-ichi’, ‘O’, and ‘P’) and S. acaule with a Maxwell 16 Instrument and Maxwell 16 Tissue DNA Purification Kits (Promega, Madison, WI, USA). SMRT sequence libraries were constructed with an SMRTbell Express Template Prep Kit (PacBio, Menlo Park, CA, USA) and used for sequencing on a PacBio Sequel system (PacBio). Genome sequence data for S. pimpinellifolium LA1670 and S. lycopersicum var. cerasiforme LA1673 were obtained from a public DNA database (DRA accession numbers DRX231405 and DRX231409)²⁵.

Kuwabara K., Harada I., Matsuzawa Y., Ariizumi T, & Shirasawa K. (2021). Organelle genome assembly uncovers the dynamic genome reorganization and cytoplasmic male sterility associated genes in tomato. Horticulture Research, 8, 250.

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DNA Extraction from Tissue and Blood

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Patient phenotypes and other clinical data were obtained from the National Cancer Institute Clinical Research Information System or patient charts. This study was approved by the Institutional Review Board of the National Cancer Institute. All patients provided written informed consent.
DNA was extracted from frozen normal or tumour tissue with Maxwell 16 Tissue DNA purification kits (Promega) using the ‘tissue' programme. Blood DNA was extracted from EDTA-anticoagulated peripheral blood samples using Maxwell 16 Blood DNA purification kits (Promega) with the ‘buffy coat' programme. DNA concentrations were determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies).

Fei S.S., Mitchell A.D., Heskett M.B., Vocke C.D., Ricketts C.J., Peto M., Wang N.J., Sönmez K., Linehan W.M, & Spellman P.T. (2016). Patient-specific factors influence somatic variation patterns in von Hippel–Lindau disease renal tumours. Nature Communications, 7, 11588.

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DNA Extraction from Plant Leaves

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Genomic DNAs for both genotyping and whole genomic sequencing were extracted from leaves using a Maxwell 16 Tissue DNA Purification kits according to the manufacturer’s protocol (Promega, Madison, USA).

Damayanti F., Lombardo F., Masuda J.I., Shinozaki Y., Ichino T., Hoshikawa K., Okabe Y., Wang N., Fukuda N., Ariizumi T, & Ezura H. (2019). Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Frontiers in Plant Science, 10, 1234.

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Whole-genome Sequencing of P. aeruginosa PAO1

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Whole-genome sequencing was performed following genomic DNA extraction from wild-type P. aeruginosa PAO1 and evolved PAO1 isolates using the Maxwell instrument and Maxwell 16 tissue DNA purification kits (Promega) according to the manufacturer’s instructions. Briefly, 3 ml of fresh overnight culture was pelleted by centrifugation, and the pellet was resuspended in 300 μl 4 M UltraPure guanidine isothiocyanate (ThermoFisher Scientific) and added directly into the DNA purification kits. Eluted DNA was stored at −20°C. Whole-genome sequencing was performed at the Cardiff School of Biosciences Genomics Research Hub. DNA was prepared for sequencing using the NEBNext Ultra II DNA library prep kit for Illumina and NEBNext multiplex oligonucleotides for Illumina (New England BioLabs Inc.). Sequencing was carried out on an Illumina NextSeq500 using a NextSeq 500/550 Mid Output v2 kit (300 cycles), giving, on average, 135-bp paired-end reads. Approximately 2.9 million reads (range: 2.5 to 3.4 million) were yielded per sample, corresponding to an average coverage depth of approximately 125× (range: 106 to 142×).

Oakley J.L., Weiser R., Powell L.C., Forton J., Mahenthiralingam E., Rye P.D., Hill K.E., Thomas D.W, & Pritchard M.F. (2021). Phenotypic and Genotypic Adaptations in Pseudomonas aeruginosa Biofilms following Long-Term Exposure to an Alginate Oligomer Therapy. mSphere, 6(1), e01216-20.

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Genomic DNA Extraction from Tissues

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Genomic DNA was extracted from embryonic brains, embryonic liver, placentas, maternal liver, EB cells of mouse origin and brains from human fetuses with NTD using the Maxwell^® 16 Tissue DNA Purification kits (Promega, Madison, WI, USA) according to the manufacturer’s instructions.

Wang L., Chang S., Wang Z., Wang S., Huo J., Ding G., Li R., Liu C., Shangguan S., Lu X., Zhang T., Qiu Z, & Wu J. (2017). Altered GNAS imprinting due to folic acid deficiency contributes to poor embryo development and may lead to neural tube defects. Oncotarget, 8(67), 110797-110810.

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Latitudinal Variation in Sunflower Traits

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For this study we selected natural populations of common sunflower from across the same latitudinal cline where others have demonstrated continuous variation in certain traits such as flowering time (Blackman et al., 2011 , Ranathunge et al., 2018) , seed oil content (Linder 2000) , seed mass (Ranathunge et al., 2018 ), and plant height (McAssey et al., 2016 , Ranathunge et al., 2018) . Seeds from 17 natural populations of common sunflower transecting the latitudinal cline from Saskatchewan to Oklahoma were collected and grown in either laboratories or greenhouses (Figure 2; Supplementary Table S1). DNA was isolated from leaf tissue with Maxwell 16 tissue DNA purification kits (Promega, WI, USA), Qiagen DNeasy Plant Mini Kits (Valencia, CA), or the CTAB protocol (Murray & Thompson, 1980) .

Ranathunge C., Chimahusky M.E, & Welch M.E. (2022). A comparative study of population genetic structure reveals patterns consistent with selection at functional microsatellites in common sunflower. Molecular genetics and genomics : MGG, 297(5).

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Quantitative DNA Methylation Analysis

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Representative tumor tissue with highest available tumor content was chosen for DNA extraction. The Maxwell® 16 FFPE Plus LEV DNA Kit or the Maxwell® 16 Tissue DNA Purification Kit (for frozen tissue) was applied on the automated Maxwell device (Promega, Madison, WI, USA) according to the manufacturer’s instructions. All tumors had a total amount of >100 ng DNA and were suitable for the array-based DNA methylation analysis. All tumors were subjected to Illumina Infinium HumanMethylation450 (450k) BeadChip or the successor EPIC/850k BeadChip (Illumina, San Diego, USA) analysis at the Genomics and Proteomics Core Facility of the German Cancer Research Center (DKFZ) Heidelberg. DNA methylation data were normalized by performing background correction and dye bias correction (shifting of negative control probe mean intensity to zero and scaling of normalization control probe mean intensity to 20000, respectively). Probes targeting sex chromosomes, probes containing multiple single nucleotide polymorphisms and those that could not be uniquely mapped were removed. Probes from the EPIC array were excluded if the predecessor Illumina Infinium 450k BeadChip did not cover them, thereby making data generated by both 450k and EPIC feasible for subsequent analyses. In total, 438370 probes were kept for analysis.

Koelsche C., Kriegsmann M., Kommoss F.K., Stichel D., Kriegsmann K., Vokuhl C., Grünewald T.G., Romero-Pérez L., Kirchner T., de Alava E., Diaz-Martin J., Hartmann W., Baumhoer D., Antonescu C.R., Szuhai K., Flucke U., Dirksen U., Pfister S.M., Jones D.T., Mechtersheimer G, & von Deimling A. (2019). DNA methylation profiling distinguishes Ewing-like sarcoma with EWSR1-NFATc2 fusion from Ewing sarcoma. Journal of cancer research and clinical oncology, 145(5), 1273-1281.

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Genotyping Atf3 Knockout Mice

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The genomic DNA of Atf3 KO mice was prepared from their tail using Maxwell 16 Tissue DNA Purification Kit (Promega). Each target gene sequence was amplified by PCR with GoTaq (Promega) by the primers Fwd08 and Rev15, and sequenced. For ATF3-21a, two additional primers were used to confirm the sequence; Fwd11: CTG GAA CTG GAG TTT CAG AG and Rev18: ATG GGT CAG CAG TTT ACA A.

Deviatiiarov R., Ishikawa K., Gazizova G., Abe T., Kiyonari H., Takahashi M., Gusev O, & Sunagawa G.A. (2021). Integrative transcription start site analysis and physiological phenotyping reveal torpor-specific expression program in mouse skeletal muscle. Communications Biology, 4, 1290.

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Genotyping Genomic DNA Deletions

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Genomic DNA was extracted from cells using a Maxwell 16 Tissue DNA Purification Kit on a Maxwell 16 Instrument (Promega) according to manufacturer's instructions. To recognize the presence of the wild-type and deletion polymorphism alleles, we conducted polymerase chain reactions (PCR) using the discriminating primers (forward 5′-AATACCACAGAGGCCCA-CAG-3′ and reverse 5′-GCCTGAAGGTGCTGAGAAAG-3′). Genomic DNAs were amplified using a Veriti Thermal Cycler (Applied Biosystems) with REDAccuTaq LA DNA Polymerase (Sigma). The PCR amplicons for the wild-type (4226 bp) and deletion (1323 bp) alleles were separated by agarose gel electrophoresis.

Arai S., Takeuchi S., Fukuda K., Tanimoto A., Nishiyama A., Konishi H., Takagi A., Takahashi H., Ong S.T, & Yano S. (2020). Resminostat, a histone deacetylase inhibitor, circumvents tolerance to EGFR inhibitors in EGFR-mutated lung cancer cells with BIM deletion polymorphism. The journal of medical investigation : JMI, 67(3.4).

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Staphylococcal Whole Genome Sequencing

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Staphylococcal DNA was prepared for WGS using lysostaphin or achromopeptidase, the Maxwell^® 16 Tissue DNA Purification kit (Promega Corporation, Madison, WI) and the Genomic DNA Clean & Concentrator^™ kit (Zymo Research Corporation, Irvine, CA), performed according to the manufacturers’ instructions. WGS was performed on a MiSeq (Illumina, Inc., San Diego, CA) using the paired-end mode (2x250) in the Mayo Clinic Medical Genome Facility. Reads were trimmed using Trimmomatic. FASTQ files were imported into SeqSphere+ software version 2.3 (Ridom GmbH) for analysis. de novo assembly and application of a S. aureus cgMLST scheme was done using SeqSphere+ with default parameters and with 1,861 queried target genes, as described previously [19 (link)]. If genomes did not contain ≥95% of the 1,861 cgMLST target genes with a minimum of ten times global local coverage, WGS was repeated.

Park K.H., Greenwood-Quaintance K.E., Uhl J.R., Cunningham S.A., Chia N., Jeraldo P.R., Sampathkumar P., Nelson H, & Patel R. (2017). Molecular epidemiology of Staphylococcus aureus bacteremia in a single large Minnesota medical center in 2015 as assessed using MLST, core genome MLST and spa typing. PLoS ONE, 12(6), e0179003.

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