Log In Sign up
The largest database of trusted experimental protocols

Tbx21

Manufactured by Jackson ImmunoResearch
Visit Supplier
Sourced in Montenegro

Tbx21−/−, also known as T-bet knockout, is a genetically engineered mouse model. It lacks the Tbx21 gene, which is a key transcription factor involved in the development and function of T helper 1 (Th1) cells. This model is commonly used in immunological research to study the role of Th1 cells in various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

C57bl 6, by Jackson ImmunoResearch (6 mentions) Cd11c cre, by Jackson ImmunoResearch (2 mentions) Ifn γ, by Jackson ImmunoResearch (2 mentions) Rag2 γc mice, by Taconic Biosciences (1 mentions) Irf8flox flox, by Jackson ImmunoResearch (1 mentions) Tbx21flox flox, by Jackson ImmunoResearch (1 mentions) Wild type c57bl 6, by Jackson ImmunoResearch (1 mentions) Tcrα mice, by Jackson ImmunoResearch (1 mentions) αox40 antibody clone ox 86, by BioXCell (1 mentions) Ifnγr1, by Jackson ImmunoResearch (1 mentions) C57bl 6j wt, by Jackson ImmunoResearch (1 mentions) Ifngr1, by Jackson ImmunoResearch (1 mentions) Cd4 cre mice, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Charles River Laboratories (1 mentions) Bcl6fl fl, by Jackson ImmunoResearch (1 mentions) Balb c mice, by Charles River Laboratories (1 mentions) Rag2 il2rg mice, by Taconic Biosciences (1 mentions) High fat diet (hfd), by TestDiet (1 mentions)

10 protocols using tbx21

1

Mouse Strains for Immunological Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6, Rag2-/-, Tbx21-/-, CD11c-Cre, Irf8flox/flox, and Tbx21flox/flox mice were obtained from Jackson Laboratory (Bar Harbor, ME) and Rag2-/-γc-/- mice were obtained from Taconic (Rensselaer, NY). All control and experimental mice were age- and sex-matched within all individual experiments. This study included both male and female mice, and the data derived from male and female mice identified no sex-specific differences in the performed experiments.
López-Yglesias A.H., Burger E., Camanzo E., Martin A.T., Araujo A.M., Kwok S.F, & Yarovinsky F. (2021). T-bet-dependent ILC1- and NK cell-derived IFN-γ mediates cDC1-dependent host resistance against Toxoplasma gondii. PLoS Pathogens, 17(1), e1008299.
+ Open protocol
+ Expand
2

Transgenic Malaria Parasite Generation

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Male and female C57BL/6 wild type, Tbx21−/−, Rag1−/− and Tcrα−/− mice (6–8 weeks, 16–21g) were purchased from Jackson Laboratories. SMARTA mice were generously provided by Dorian McGavern (Scripps) via Allan Zajac (UAB). The University of Oklahoma Health Sciences Center and University of Iowa Institutional Animal Care and Use Committees approved all experiments. Plasmodium yoelii (clone 17XNL, BEI Resources/MR4/ATCC) was inoculated into mice intravenously. To generate the T cell epitope tagged transgenic parasites (see Fig. S1 and Table S1), a dispensable genetic locus (“p230p”) was stably targeted with an expression cassette of Hep17 protein tagged with the GP61–80 CD4 T cell epitope (amino acids 61–80 from LCMV glycoprotein). Standard methods (Jongco et al., 2006 (link), Lindner et al., 2013 (link)) were used to create and integrate a linearized pDEF construct, select transgenic parasites, and obtain two pure, clonal populations. Parasitemia was measured using flow cytometry as previously described (Malleret et al., 2011 (link)). LCMV clone 13 (2×106 plaque forming units) was administered i.p. 50 µg of rIgG or α-OX40 antibody (clone OX86, BioXCell) were administered i.p. on the days indicated in figure legends.
Zander R.A., Vijay R., Pack A.D., Guthmiller J.J., Graham A.C., Lindner S.E., Vaughan A.M., Kappe S.H, & Butler N.S. (2017). Th1-like Plasmodium-specific memory CD4+ T cells unexpectedly support humoral immunity. Cell reports, 21(7), 1839-1852.
+ Open protocol
+ Expand
3

Genetic Knockout Mice for Immunology

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6, Tbx21−/−, IFN-γ−/−, and RAG1−/− and RAG2−/− mice were obtained from Jackson Laboratory. Control and experimental mice were age-matched within all individual experiments. All mice were maintained at the American Association of Laboratory Animal Care-accredited animal facility at the University of Rochester Medical Center.
López-Yglesias A.H., Burger E., Araujo A., Martin A.T, & Yarovinsky F. (2018). T-bet-independent Th1 response induces intestinal immunopathology during Toxoplasma gondii infection. Mucosal immunology, 11(3), 921-931.
+ Open protocol
+ Expand
4

Murine Malaria Infection Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6J WT, Il10−/−, Il10Rβ−/−, µMT, Tbx21−/−, and Ifnγr1−/− (8 weeks old) mice were purchased from Jackson Laboratories. The OUHSC IACUC approved all experiments. Plasmodium yoelii (clone 17XNL, obtained from MR4, ATCC) was routinely passaged through mosquitos. Infections were initiated by a serial transfer of 106P. yoelii parasite-infected red blood cells (pRBCs) i.v. Parasitemia was measured by detecting RNA/DNA content in Ter119+ red blood cells via flow cytometry. For IL-10R blockade studies, WT mice were injected i.p. with 200 µg of α-IL-10R (1B1.3A, Bioxcell) or rIgG every three days starting on day 1 p.i. For studies neutralizing IFN-γ, Il10−/− mice were injected i.p. with 500 µg of α-IFN-γ (XMG1.2, Bioxcell) or rIgG every two days beginning one day prior to infection.
Guthmiller J.J., Graham A.C., Zander R.A., Pope R.L, & Butler N.S. (2016). IL-10 is essential for the generation of germinal center B cell responses and anti-Plasmodium humoral immunity. Journal of immunology (Baltimore, Md. : 1950), 198(2), 617-622.
+ Open protocol
+ Expand
5

Mouse Strains for Immunological Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6, OTII TCR transgenic, Rag1–/–, Ifngr1–/–, Il27ra–/–, and Tbx21–/– mice were purchased from the Jackson Laboratories and maintained in our facilities. Cd4(ΔRunx3) mice were described previously (Reis et al., 2013 (link)). Dominant-negative Raralsl/lsl, Runx3-YFP, and Zbtb7b-GFP reporter mice were generously provided by R. Noelle (Rajaii et al., 2008 (link)), D. Littman, and I. Taniuchi, respectively. Several of these lines were interbred in our facilities to obtain the final strains described in the text. Mice were maintained at The Rockefeller University animal facilities under specific pathogen-free conditions, and sentinel mice from the Rag1–/– mouse colony were tested to be negative for Helicobacter spp. and C. rodentium. Mice were used at 7–12 weeks of age for all experiments except when otherwise indicated. Animal care and experimentation were consistent with the NIH guidelines and were approved by the Institutional Animal Care and Use Committee at The Rockefeller University.
Reis B.S., van Konijnenburg D.P., Grivennikov S.I, & Mucida D. (2014). Transcription Factor T-bet Regulates Intraepithelial Lymphocyte Functional Maturation. Immunity, 41(2), 244-256.
+ Open protocol
+ Expand
6

Genetically Modified Mouse Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 and BALB/C mice were purchased from Beijing Vital River Laboratory Animal Technology (licensed from Charles River). Bcl6fl/fl, Tbx21−/−, and Cd4-Cre mice were purchased from Jackson Laboratories. Bcl6fl/fl mice were bred with Cd4-Cre mice to generate Bcl6fl/flCd4-Cre mice. IFN-γ-YFP reporter mice were provided by Dr. Z.N. Yin (Jinan University, Guangzhou, China). CXCR5-GFP reporter mice, Tbx21fl/flCd4-Cre mice, and Ifng−/− mice were provided by Dr. L.L. Ye (Third Military Medical University, Chongqing, China). Ifngr1−/− mice were provided by Dr. X.Z. Liang (Institut Pasteur of Shanghai, Shanghai, China).
Liang H., Tang J., Liu Z., Liu Y., Huang Y., Xu Y., Hao P., Yin Z., Zhong J., Ye L., Jin X, & Wang H. (2019). ZIKV infection induces robust Th1-like Tfh cell and long-term protective antibody responses in immunocompetent mice. Nature Communications, 10, 3859.
+ Open protocol
+ Expand
7

Foxo1 and Foxo3 Conditional Knockout Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
All mice were bred and housed in specific pathogen-free conditions in accordance with Ohio State University Animal Care and Use Guidelines as well as French and European guidelines for animal care. All animal work was approved by The Ohio State University Animal Care and Use Committee, as well as Marseille’s ethical committee for animal use.
C57BL/6 Foxo1fl/fl and Foxo3f/f mice were a kind gift from Dr. Ming Li at the Memorial Sloan-Kettering Cancer Center as well as Dr. Ronald A. DePinho at the Dana Farber Cancer Institute. Congenic C57BL/6 CD45.1 and Tbx21−/− were purchased from The Jackson Laboratory. Rag2−/−Il2rg−/− mice were purchased from Taconic Biosciences. All mice used were 8- to 12-week-old.
Deng Y., Kerdiles Y., Chu J., Yuan S., Wang Y., Chen X., Mao H., Zhang L., Zhang J., Hughes T., Deng Y., Zhang Q., Wang F., Zou X., Liu C.G., Freud A.G., Li X., Caligiuri M.A., Vivier E, & Yu J. (2015). Transcription Factor Foxo1 Is a Negative Regulator of NK Cell Maturation and Function. Immunity, 42(3), 457-470.
+ Open protocol
+ Expand
8

Genetic Mouse Models for Metabolic Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6, Rag1-/- and Tbx21-/- (formal gene name Tbx21) mice were purchased from The Jackson Laboratory (Bar Harbor, ME) Rag1-Tbet DKO mice were obtained through crossbreeding of Rag1−/− mice with Tbx21−/− mice. Genotyping for Rag1-Tbet DKO was performed by Transnetyx. Mice were fed either standard chow (normal diet, ND) or a lard-based high fat diet (HFD). The ND, consisting of 4.09 kcal/gram,13.4% kJ/fat, was purchased from Lab Diet (St. Louis, MO). The HFD, consisting of 5.10 kcal/gram, 60% kJ/fat, was purchased from TestDiet (St. Louis, MO). ND was started at week four of life and maintained for 12-15 weeks for the ND group. For the HFD group experiment, HFD feeding was started between weeks five and seven of life. HFD continued for 12 weeks. All mice were bred and maintained within a pathogen-free facility in the Division of Comparative Medicine at Georgetown University Medical Center, with a standard 12-hour light-dark cycle. All procedures on animal subjects were fully approved by the Georgetown University Institutional Animal Care and Use Committee (protocol #2016-1351).
Kang J., Liggett J.R., Patil D., Ranjit S., Loh K., Duttargi A., Cui Y., Oza K., Frank B.S., Kwon D., Kallakury B., Robson S.C., Fishbein T.M., Cui W., Khan K, & Kroemer A. (2022). Type 1 Innate Lymphoid Cells Are Proinflammatory Effector Cells in Ischemia-Reperfusion Injury of Steatotic Livers. Frontiers in Immunology, 13, 899525.
+ Open protocol
+ Expand
9

Characterization of Immune Cells in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6, RAG2 -/-, Tbx21 -/-, CD11c-Cre, and Irf8 flox/flox mice were obtained from Jackson Laboratory (Bar Harbor, ME). All control and experimental mice were age-and sex-matched within all individual experiments. This study included both male and female mice, and the data derived from male and female mice identified no sex-specific differences in the performed experiments.
López-Yglesias A.H., Burger E., Camanzo E., Martin A.T., Araujo A., Kwok S.F., & Yarovinsky F. (2020). ILC1-derived IFN-γ mediates cDC1-dependent host resistance againstToxoplasma gondii.
+ Open protocol
+ Expand
10

Genetic Knockout Mice for Immunology

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6, Tbx21−/−, IFN-γ−/−, and RAG1−/− and RAG2−/− mice were obtained from Jackson Laboratory. Control and experimental mice were age-matched within all individual experiments. All mice were maintained at the American Association of Laboratory Animal Care-accredited animal facility at the University of Rochester Medical Center.
López-Yglesias A.H., Burger E., Araujo A., Martin A.T, & Yarovinsky F. (2018). T-bet-independent Th1 response induces intestinal immunopathology during Toxoplasma gondii infection. Mucosal immunology, 11(3), 921-931.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!