The samples were subjected to differential and scanning calorimetry (DSC), thermogravimetry derivative (TG/DTG). The DSC curves were obtained on DSC-50 cell (Shimadzu) using aluminum sample holder with 2 mg of sample in an atmosphere of nitrogen (50 mL/min−1) and heating rate of 10°C min−1 in the temperature range from 25 to 500°C. The DSC cell was checked with indium (RM 156.6°C, ΔHmel = 28.54 Jg-1) and zinc (PF 419.6°C). The TG/DTG curves were obtained in thermobalance model TGA 50 (Shimadzu) using a temperature range of 25–900°C in a platinum sample holder with ~3 mg of sample under dynamic nitrogen atmosphere (50 mL/min−1) and heating rate of 10°C/min−1. The thermobalance was verified with CaC2O4·H2O.
Dsc 50 cell
Manufactured by Shimadzu
Sourced in Japan
The DSC-50 cell is a thermal analysis instrument used to measure the heat flow associated with physical and chemical changes in a material as a function of temperature or time. It provides information about the thermal properties of a sample, such as phase transitions, glass transitions, and reactions.
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Lab products found in correlation
Inspect f50 microscope, by Thermo Fisher Scientific (2 mentions)
Tga 50 thermobalance, by Shimadzu (1 mentions)
Tga 50, by Shimadzu (1 mentions)
Tga 51 thermogravimetric analyzer, by Shimadzu (1 mentions)
870 kf titrino plus, by Metrohm (1 mentions)
Jsm 6390lv, by JEOL (1 mentions)
Cary 630, by Agilent Technologies (1 mentions)
Zetasizer nano s, by Malvern Panalytical (1 mentions)
Malvern zetasizer nano s, by Malvern Panalytical (1 mentions)
Cary 630 spectrophotometer, by Agilent Technologies (1 mentions)
Libra s22, by Harvard Bioscience (1 mentions)
Rotina 380 r benchtop, by Hettich (1 mentions)
10 protocols using dsc 50 cell
1
Thermal Characterization of Samples
2
Thermal Characterization of Materials by DSC and TGA
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Differential scanning calorimetry (DSC) curves were obtained by a DSC-50 cell (Shimadzu, Kyoto, Japan) using aluminum crucibles. Approximately 2 mg of samples were put into these crucibles, and the measurements were made under a dynamic nitrogen atmosphere (50 mL/min) at a heating rate of 10 °C/min in the temperature range 25–600 °C. The DSC cell was calibrated with indium (melting point (m.p.) = 156.6 °C and fusion enthalpy (ΔHfus.) = 28.54 J/g) and zinc (m.p. = 419.6 °C). Thermogravimetric (TG/DTG) curves were obtained with the TGA-50 thermobalance (Shimadzu, Kyoto, Japan) using platinum crucibles that contained roughly 3 mg of a sample. These measurements were made under a dynamic nitrogen atmosphere (50 mL/min) at a heating rate of 10 °C/min in the temperature range 25–900 °C.
3
Thermal Analysis of Material Samples
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Differential scanning calorimetry (DSC) thermal analysis was carried out in a DSC-50 cell Shimadzu® (Tokyo, Japan) using approximately 2 mg of sample in aluminum crucibles under dynamic nitrogen atmosphere (50 mL·min−1) and with a heating rate of 10 °C·min−1 at a temperature range of 30–500 °C.
Thermogravimetry (TG) was obtained using a TGA-50 Shimadzu® (Tokyo, Japan) in the temperature range of 30–900 °C, using alumina crucibles with approximately 2 mg of samples under dynamic nitrogen atmosphere (50 mL·min−1) and with a heating rate of 10 °C·min−1.
Thermogravimetry (TG) was obtained using a TGA-50 Shimadzu® (Tokyo, Japan) in the temperature range of 30–900 °C, using alumina crucibles with approximately 2 mg of samples under dynamic nitrogen atmosphere (50 mL·min−1) and with a heating rate of 10 °C·min−1.
4
Thermal and Morphological Analysis of Drug Compounds
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The differential scanning calorimetry (DSC) curves (OEFC, β-CD, MF, MA, and CE) were obtained using a DSC-50 cell (Shimadzu, Kyoto, Japan), at a heating rate of 10 °C/min, at 25–500 °C, under an N2 dynamic atmosphere (50 mL/min). An aluminum (Al) capsule containing approximately 2 mg of the sample was used in this assay. For the TG/DTG (thermogravimetry/derivative thermogravimetry) assays, a TGA-51 thermogravimetric analyzer (Shimadzu, Kyoto, Japan) was used at a temperature range of 25–500 °C, under a dynamic N2 atmosphere (50 mL/min). A platinum crucible (Pt) containing approximately 2 mg of the sample was used in this assay. For the MeV analysis, the samples were mounted in aluminum tubes, metallized with gold beams, and visualized in an electronic microscope (model JSM-6390-LV; JEOL, Peabody, MA, USA) under a voltage acceleration of 12 kV. The moisture content (OEFC, β-CD, MF, MA, and CE) was determined using the Karl Fischer method with the aid of a Titrino Plus KF 870 (Metrohm, Herisau, Switzerland), using methanol (Fluka, St. Louis, MO, USA) as the titration solution. The analyses were performed in triplicates [42 (link)].
5
DSC Thermal Analysis of Samples
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DSC thermal analysis was carried out in a DSC-50 cell Shimadzu® (Tokyo, Japan) using approximately 2 mg of sample in aluminum crucibles under dynamic nitrogen atmosphere (50 mL·min−1) and heating rate of 10 °C∙min−1 at a temperature range of 30–500 °C. The temperature and heat flow of the DSC instrument was calibrated with indium (melting point = 157.5 °C and ∆H = 26.7 J·g−1).
6
Thermal Analysis of Quercetin Formulations
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DSC curves of quercetin free and suspension were obtained in a DSC-50 cell (Shimadzu) using aluminum crucibles with about 2 mg of samples, under dynamic nitrogen atmosphere (50 mL min−1) and heating rate of 10 °C min−1 in the temperature range from 25 to 600 °C. The DSC cell was calibrated with indium (mp 156.6 °C; ΔHmelting = 28.54 J g−1) and zinc (mp 419.6 °C).
7
Physicochemical Characterization of Nanoparticles
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Dynamic Light Scattering (DLS) technique was used to obtain the mean size and polydispersity index (PDI) of the nanoparticles. Zeta potentials of the nanoparticles were measured by a Malvern Zetasizer Nano S. Scanning electron microscopy (SEM) images were taken using a Inspect F50 microscope (FEI Company) with field emission guns. Liquid samples were dropped on aluminum stubs covered with carbon tape, dried at 40 • C for 2 h, metalized with gold in the secondary electron mode, and then analyzed at 20 kV. Lyophilized nanoparticles and raw materials (HRP, IAA, CS and HA) were analyzed by Fourier-transformed infrared spectroscopy (FTIR) in spectrophotometer Cary 630 (Agilent Technologies), equipped with a zinc selenide crystal (ZnSe) and ATR (total attenuated reflection) device. Thermogravimetric analysis (TGA) was performed in SDT equipment model Q600 (TA Instruments): heating flow 10 • C/min (35 • C-600 • C) under nitrogen atmosphere (10 mL/ min). Differential scanning calorimetry (DSC) was performed in DSC-50 cell (Shimadzu): heating flow 10 • C/min (30 • C-500 • C) under nitrogen atmosphere (50 mL/min).
8
Thermal Analysis of Samples
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Thermogravimetric analysis (TGA) was carried out using an SDT equipment model Q600 (TA Instruments -Delaware, USA). About 5.0 mg of the samples were supported in an alumina crucible and analyzed using a flow rate of 10 mL/min, under inert nitrogen and heating flow of 10 • C/min, from 35 • C to 500 • C. Differential scanning calorimetry (DSC) was performed through the DSC-50 cell (Shimadzu -Kyoto, Japan), with a flow rate of 50 mL/min, under inert nitrogen, and heating rate of 10 • C/min from 35 to 500 • C. Samples (2.0 mg) were analyzed in closed aluminum capsules and an empty aluminum capsule was used as reference.
9
Nanoparticle Characterization Protocol
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For the preparation of nanoparticles: Magnetic stirrer (C-MAG HS 7, IKA -Staufen, Germany), pHmeter (D-22, Digimed -São Paulo, Brazil). Determination of Particle size, polydispersity index, and zeta potential: Malvern Zetasizer Nano S (Malvern Instruments, Worcestershire, UK). Enzymatic activity: UV/Vis Spectrophotometer (Libra S22, Biochrom -Cambridge, UK), centrifuge (Rotina 380 R Benchtop, Andreas Hettich GmbH & Co. KG -Tuttlingen, Germany). For Fourier transform infrared spectroscopy (FTIR), Thermogravimetric and Differential scanning calorimetry (DSC) analyzes were used: Cary 630 Spectrophotometer (Agilent Technologies -California, USA), SDT equipment model Q600 (TA Instruments -Delaware, USA), DSC-50 cell (Shimadzu -Kyoto, Japan), respectively. For Scanning electron microscopy (SEM) was used an Inspect F50 microscope (FEI Company -Tokyo, Japan).
10
Thermal Analysis of Inclusion Complexes
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The EOHM, b-CD, PM and of inclusion complexes samples were subjected to DSC test. The DSC curves were obtained using a DSC-50 cell, from Shimadzu, using a heating rate of 10 C/min. The DSC curves were obtained between 25 and 500 C under a dynamic atmosphere of N 2 (50 mL/min), employing aluminum capsules (Al) containing $2 mg of the samples. The DSC cell was calibrated with indium (melting point 156.6 C; DH fus = 28.54 J/g) and zinc (melting point 419.6 C).
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