Anti cd5 ly 1 microbeads

Bone marrow chimeras were generated essentially as previously described (24 (link)). Briefly, suspensions of bone marrow, obtained from the tibias and femurs of CD45.1 IL-10R^+/+ and CD45.2 IL-10R^−/− mice were depleted of T cells using anti-CD5 (Ly-1) microbeads (Miltenyi Biotec; Auburn, CA). RAG-1^−/− recipient mice were exposed to two doses of radiation (~500 rads each) from a ¹³⁷Cs source, given 3–4 hours apart. These irradiated recipients were then reconstituted by i.v. injection of 5×10⁶ CD45.1 IL-10R^+/+ T cell depleted bone marrow cells and an equal number of CD45.2 IL-10R^−/− cells. Mice were provided chlorinated acidified water containing neomycin for 6 weeks following reconstitution.

Bone marrow chimeras were generated essentially as previously described (29 (link)). Briefly, suspensions of bone marrow, obtained from the tibias and femurs of CD45.1 IL-21R^+/+, CD45.2 B6, and CD45.2 IL-21R^−/− mice were depleted of T cells using anti-CD5 (Ly-1) microbeads (Miltenyi Biotec; Auburn, CA). RAG-1^−/− recipient mice were exposed to two doses of radiation (~500 rads each) from a ¹³⁷Cs source, given 3~4 hours apart. Mice were then reconstituted by i.v. injection of 7×10⁶ CD45.1 IL-21R^+/+ T cell depleted bone marrow and equal numbers of CD45.2 IL-21R^+/+ or CD45.2 IL-21R^−/− cells. Mice were provided chlorinated acidified water containing sulfamethoxazole, trimethoprim, and neomycin for 6 weeks.