Trimethylsilyldiazomethane tmsd

Manufactured by Merck Group

Sourced in United States

Trimethylsilyldiazomethane (TMSD) is a chemical reagent used in organic synthesis. It is a source of the trimethylsilyl (TMS) group and a diazo functionality. TMSD is commonly utilized in various organic reactions, including methylation, silylation, and cyclopropanation.

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2 protocols using trimethylsilyldiazomethane tmsd

Comprehensive Lipid Standards for HPLC Analysis

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HPLC-grade acetonitrile, methanol, water, and 2-propanol were purchased from J.T. Baker (Avantor Performance Material, Inc., Center Valley, PA, USA). Fluka Analytical HPLC-grade formic acid, chloroform, ammonium formate, and trimethylsilyldiazomethane (TMSD) were purchased from Sigma-Aldrich. All lipid standards (Avanti Polar Lipids, Inc., Alabaster, AL, USA) used in this study were as follows: phosphatidylcholine (PC (10:0–10:0), PE (10:0–10:0), PG (10:0–10:0), PI (8:0–8:0), PS (10:0–10:0), PA (10:0–10:0), LPC (C13:0), LPE (C14:0), LPG (C14:0), LPI (C13:0), LPS (C17:1), lysophosphatidic LPA (C17:0), SM (d18:1–12:0), Cer (d18:1–12:0), dCer (d18:0–12:0), SO (C17:1), SA (C17:0), Cer1P (d18:1–12:0), dCer1P (d18:0–16:0), SO1P (C17:1), and SA1P (C17:0). TG (11:1–11:1–11:1), DG (8:0–8:0), and ChE (10:0) were purchased from Larodan Fine Chemicals AB (Malmö, Sweden).

Mok H.J., Shin H., Lee J.W., Lee G.K., Suh C.S., Kim K.P, & Lim H.J. (2016). Age-Associated Lipidome Changes in Metaphase II Mouse Oocytes. PLoS ONE, 11(2), e0148577.

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Immunofluorescence Staining of CRB3 Protein

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2,4-Dichlorophenoxyacetic acid (2,4-D) (CAS no. 94-75-7), para-aminohippuric acid (PAH) (CAS no. 61-78-9), triclopyr (CAS no. 55335-06-3), trimethylsilyldiazomethane (TMSD) (CAS no. 18107-18-1), N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) (CAS no. 77377-52-7), ethyl acetate, toluene, lucifer yellow, pilocarpine, and other general laboratory chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-CRB3 rat primary monoclonal antibody was purchased from MyBioSource (Cat # MBS588104) and goat anti-rat IgG secondary antibody-Alexa Fluor 488 conjugate was purchased from Invitrogen (Cat # A11006).

Carver Z.A., Han A.A., Timchalk C., Weber T.J., Tyrrell K.J., Sontag R.L., Luders T., Chrisler W.B., Weitz K.K, & Smith J.N. (2018). Evaluation of non-invasive biomonitoring of 2,4-Dichlorophenoxyacetic acid (2,4-D) in saliva. Toxicology, 410, 171-181.

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