Alexa fluor 488 direct labeled goat anti rabbit a11034

Antibodies raised against human macroH2A1 and macroH2A2 were described previously (Chadwick and Willard 2001 (link); Chadwick and Willard 2002 (link)). Mouse monoclonals to human HP1 isoforms HP1-alpha (Cat. No. MAB3584), HP1-beta (Cat. No. MAB3448) and HP1-gamma (Cat. No. MAB3450) were all originally obtained from Chemicon International, but are now available from Millipore Sigma. Mouse monoclonal anti-histone H1 (Cat. No. Sc-8030) and rabbit polyclonal to HMG-I/Y (Cat. No. Sc-1564) were obtained from Santa Cruz Biotechnology. Rabbit polyclonal antibodies to H3K4me2 (Cat. No. 07–030), H3K27me3 (Cat. No. 07–449) and H3K9me3 (Cat. No. 07–523) were obtained from Millipore Sigma. Mouse monoclonal antibodies to H3K27me2/3 (Cat. No. 39535) were obtained from Active Motif. Images generated using the mouse H3K27me3 or rabbit H3K27me3 are indicated in the captions. Rabbit monoclonal antibodies to H2AK119Ub1 (Cat. No. 8240S) was obtained from Cell Signaling Technology. Rabbit polyclonal antibodies to SMCHD1 (Cat. No. A302–872A) were obtained from Bethyl Laboratories Inc. Alexa Fluor 555 direct-labeled goat anti-rabbit (A21429) and goat anti-mouse (A21424), and Alexa Fluor 488 direct-labeled goat anti-rabbit (A11034) and goat anti-mouse (A11029) were obtained from Thermo Fisher Scientific.

Antibodies raised against human macroH2A1 were described previously (Chadwick and Willard 2001 (link)). Mouse monoclonal to HP1-gamma (HP1γ) (Cat. No. sc-398562), ATRX (Cat. No. sc-55584) and CTCF (Cat. No. sc-271474) were obtained from Santa Cruz Biotechnology. Rabbit polyclonal antibodies to EZH2 (Cat. No. 07-400), H3K4me2 (Cat. No. 07-030) and H3K9me3 (Cat. No. 07-523) were obtained from Millipore Sigma. Mouse monoclonal antibodies to H3K27me2/3 (Cat. No. 39535) were obtained from Active Motif. Rabbit monoclonal antibodies to H2AK119Ub1 (Cat. No. 8240S) was obtained from Cell Signaling Technology. Rabbit polyclonal antibodies to SMCHD1 (Cat. No. A302-872A) were obtained from Bethyl Laboratories Inc. Alexa Fluor 555 direct-labeled goat anti-rabbit (A21429) and goat anti-mouse (A21424), and Alexa Fluor 488 direct-labeled goat anti-rabbit (A11034) and goat anti-mouse (A11029) were obtained from Thermo Fisher Scientific.