Hplc ms grade

Manufactured by Merck Group

Sourced in United States, Germany, Italy

HPLC MS grade is a type of laboratory equipment used for high-performance liquid chromatography (HPLC) and mass spectrometry (MS) analysis. It is designed to provide a high-quality, consistent, and pure sample environment for accurate and reliable analytical results.

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11 protocols using hplc ms grade

Quantification of Patulin in Samples by HPLC-MS

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HPLC/MS (ACQUITY ^® TQD, Waters, Milford, MA, USA) was used to detect and quantify patulin in the samples. The chromatographic separation was achieved on a C₁₈ BEH (2.1 × 100 mm, 1.7 μm) (Waters, Milford, MA, USA) column at 30 °C with a flow rate of 0.3 mL/min. The injection was made with a volume of 5 µL. Eluent A was HPLC-grade water and eluent B was acetonitrile (HPLC MS grade, Sigma-Aldrich, St. Louis, MO, USA).
Elution conditions were as follows: a 1 min isocratic passage of solvent B from 100 to 2%, a 3 min gradient increase of solvent B from 2 to 90%, 1 min isocratic passage of solvent B at 90%, a 1 min gradient decrease of solvent B from 90 to 2 and re-equilibration at 2% solvent B for 4 min.
Electrospray ionization was the ionization source. The parameters were as follows: Source temperature: 120 °C, desolvation temperature: 300 °C, Cone gas: 60 L/h, desolvatation gas flow: 800 L/h. Spectra were acquired in negative ionization selected reaction monitoring (SIR) mode with interchannel delay of 0.050 s.

Settier-Ramírez L., López-Carballo G., Hernández-Muñoz P., Fontana A., Strub C, & Schorr-Galindo S. (2021). New Isolated Metschnikowia pulcherrima Strains from Apples for Postharvest Biocontrol of Penicillium expansum and Patulin Accumulation. Toxins, 13(6), 397.

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HPLC-MS Analysis of Carbohydrates and Organic Acids

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The chemicals for the sample extractions and for the mobile phases were HPLC-MS grade, methanol and phosphoric acid were purchased from Sigma-Aldrich (Burlington, MA, USA), while sodium hydroxide was purchased from J.T. Baker (Deventer, The Netherlands). HPLC grade carbohydrate standards (glucose, fructose, and sucrose), as well as citric and malic acid were procured from Fluka (Buchs, Switzerland), while shikimic and fumaric acid were purchased from Sigma-Aldrich (Burlington, MA, USA). All utilized solvents and chemicals for MS analysis were purchased from Merck Chemicals, Carl Roth or VWR Chemicals as HPLC (purity ≥ 99.9%) or analytical grade chemicals (purity ≥ 99%). Water for the mobile phase was double distilled and purified with the Milli-Q system (Millipore, Bedford, MA, USA).

Milosavljević D., Maksimović V., Milivojević J., Djekić I., Wolf B., Zuber J., Vogt C, & Dragišić Maksimović J. (2023). Sugars and Organic Acids in 25 Strawberry Cultivars: Qualitative and Quantitative Evaluation. Plants, 12(12), 2238.

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HPLC-MS/MS Quantification of Patulin

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HPLC/MS-MS (LCMS 8040 Shimadzu) was used to detect and quantify patulin in the samples. The data were analyzed using Labsolution software (Shimadzu, Kyoto, Japan). The chromatographic separation was carried out on Kinetex 2.6 μm C18 100A 50 × 2.1 mm ID column (Phenomenex, Hong Kong, China). Column temperature was maintained at 50 °C and injection was made with a volume of 50 μL. The mobile phase A consisted of 90% water 10% mobile phase B consisted acetonitrile (HPLC MS grade, Sigma, St. Quentin Fallavier, France).
Electrospray ionization was the ionization source. The parameters were as follows: positive and negative ionization ES+ and ES-, MRM (Multiple Reaction Monitoring) mode, Desolvation line: 250 °C, Heater block: 400 °C NEB gas: 3 L/min, Drying gas: 15 L/min.

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Quantitative Analysis of Fatty Acids and Endocannabinoids

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The acetonitrile, m ethanol, chloroform, n-hexane, ethanol, acetic acid, FA standards, all HPLC/MS grade, and deferoxamine mesylate were purchased from Sigma Chemicals Co. (St. Louis, MO, USA). Ascorbic acid, potassium hydroxide, and hydrochloric acid were purchased from Carlo Erba (Milano, Italy). FA standards including lin oleic acid (18:2-n6), 20:4-n6, docosatetraenoic acid (22:4-n6), α-linolenic acid (18:3-n3), eicosapentaenoic acid (20:5-n3), docosahexaenoic acid (22:6-n3), palmitic acid (16:0), palmit oleic acid (16:1), stearic acid (18:0), and oleic acid (18:1-n9) were obtained from Sigma (Milan, Italy). Internal deuterated standards for the AEA, 2-AG, and OEA quantification by isotope dilution ([2H]⁸ AEA, [2H]⁵ 2-AG, [2H]² OEA, N-palmitoylethanolamine [2H]⁴ PEA) were purchased from Cayman Chemicals (Ann Arbor, MI, USA).

Manca C., Pintus S., Murru E., Fantola G., Vincis M., Batetta B., Moroni E., Carta G, & Banni S. (2021). Fatty Acid Metabolism and Derived-Mediators Distinctive of PPAR-α Activation in Obese Subjects Post Bariatric Surgery. Nutrients, 13(12), 4340.

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Formulation and Characterization of Eugenol

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Eugenol was procured from Santa Cruz Biotechnology (USA). Tween 80, Polyethylene Glycol (PEG), and other surfactants samples were used as a gift samples by Sun Pharma (Gurgaon, Haryana, India). Deionized water (DI) was more purified from Milli-Q water purification system (Millipore, Bedford, MA, USA) was used for purification of water to deionized water (DI). M Ethanol, Ethanol, Acetonitrile and other chemicals HPLC/MS grade (99.9% purity) were procured from Sigma Aldrich (Steinheim, Germany). Carbopol® 934 grades was used and purchased from Loba Chemie Pvt. Ltd., Mumbai, India.

Ahmad N., Ahmad F.J., Bedi S., Sharma S., Umar S, & Ansari M.A. (2019). A novel Nanoformulation Development of Eugenol and their treatment in inflammation and periodontitis. Saudi Pharmaceutical Journal : SPJ, 27(6), 778-790.

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Synthesis and Analysis of MAPS

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MAPS was synthesized in Bielefeld as previously described [10] . The synthetic procedure is easy, not expensive and very little time consuming. One needs only to be careful and work under inert atmosphere. MAPS samples should be stored under inert atmosphere to prevent slow degradation to PS. A solution of MAPS was prepared 10 -3 M in acetonitrile. Sample solutions for ESI analysis were further diluted to 5×10 -7 M in the selected solvent or solvent mixture. Solvents (HPLC-MS grade) and other chemicals (e.g. ammonia and N,N,N',N'-tetramethylethylenediamine) were purchased from Sigma-Aldrich (Milan, Italy). Any other additive was used at 10 -6 M concentration in the solutions for ESI analysis.

Corinti D., Crestoni M.E., Fornarini S., Pieper M., Niehaus K, & Giampà M. (2019). An integrated approach to study novel properties of a MALDI matrix (4-maleicanhydridoproton sponge) for MS imaging analyses. Analytical and bioanalytical chemistry, 411(4).

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Fatty Acid Quantification by GC/MS

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The derivatization agent used for the GC/MS analyses was N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) containing 1% trimethylchlorosilane (Sigma-Aldrich, USA).
The fatty acids stock solution in acetone contained lauric (5.41 mg g -1 of Lau), suberic (5.68 mg g -1 of Su), azelaic (4.04 mg g -1 of A), myristic (3.99 mg g -1 of My), sebacic (4.28 mg g -1 of Se), palmitic (4.70 mg g -1 of P), oleic (6.51 mg g -1 of O) and stearic acids (6.43 mg g -1 of S). Tridecanoic acid solution in iso-octane, 140.6 µg g -1 , was used as acidic and neutral fraction derivatization internal standard. hexadecane solution in iso-octane, 212.9 µg g -1 , was used as injection internal standard. All acids and hexadecane, purity 99%, were purchased from Sigma-Aldrich (USA). Standard solutions were used to derive calibration curves. Solvents: bi-distilled water (pesticides-grade, Carlo Erba); iso-octane, diethyl ether and acetone (HPLC-MS grade, Sigma Aldrich); n-hexane, iso-propanol and methanol (HPLC-MS grade, Fluka).

Degano I., La Nasa J., Ghelardi E., Modugno F, & Colombini M.P. (2016). Model study of modern oil-based paint media by triacylglycerol profiling in positive and negative ionization modes. Talanta, 161.

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Metabolic Flux Analysis of Palmitate Tracing

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For metabolic flux experiments, ¹³C-palmitate tracing was conducted using LC/MS at LipidALL Technologies. Cells (1 × 10⁷) were incubated for 8 h at 37 °C in RPMI-1640 medium with 10% FBS. For ¹³C-palmitate incorporation in metabolites, cells were incubated in growth medium with 50 μM [U-¹³C] palmitate for 24 h. Then, cells were quickly washed with 1×PBS and fixed with pre-cooled methanol (HPLC-MS grade, Millipore) for 30 min at −80 °C. The extraction method was referenced to a previous paper, but with modification [52 (link)]. Samples were incubated for 30 min at 1500 rpm and 4 °C and then centrifuged for 10 min at 12,000 rpm and 4 °C. The supernatant fractions were placed into clean 1.5-ml centrifuge tubes and dried using a SpeedVac. The dried extracts were dissolved with 50% acetonitrile in water and the upper layer liquids were collected for LC–MS analysis. The InfinityLab Poroshell 120 HILIC-Z column (2.1 mm × 50 mm, 2.7 μm, Agilent Technologies, Germany) was used in this study. Ultra-performance Liquid Chromatography (Agilent 1290 II, Agilent Technologies, Germany) coupled to the Quadrupole-TOF MS 5600 Triple TOF Plus, AB SCIEX, Singapore) was used to acquire metabolome data.

Tang M., Dong X., Xiao L., Tan Z., Luo X., Yang L., Li W., Shi F., Li Y., Zhao L., Liu N., Du Q., Xie L., Hu J., Weng X., Fan J., Zhou J., Gao Q., Wu W., Zhang X., Liao W., Bode A.M, & Cao Y. (2022). CPT1A-mediated fatty acid oxidation promotes cell proliferation via nucleoside metabolism in nasopharyngeal carcinoma. Cell Death & Disease, 13(4), 331.

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Metabolic Tracing of Cellular Palmitate

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Cells (2 ×10⁶) were incubated for 8 h at 37°C in RPMI 1640 medium with 10% fetal bovine serum (FBS). U-¹³C-palmitate (Sigma-Aldrich) was dissolved in ethanol to a final concentration of 20 mM, then mixed with a 10% free fatty acid-free bovine serum albumin (Sigma-Aldrich) solution at a 1:5 ratio and incubated 1 h at 37°C. Then the U-¹³C-palmitate solution was diluted in serum-containing RPMI 1640 medium to a working concentration of 50 μM. When tracing palmitate carbons, the cells were incubated with the medium indicated above. After 24 h, cells were quickly washed with 1×PBS and fixed with a pre-cooling methanol (HPLC-MS grade, Millipore) for 30 min at -80°C. Then cells were harvested, frozen on dry ice and processed for UPLC-MS/MS analysis as described below.

Tan Z., Xiao L., Tang M., Bai F., Li J., Li L., Shi F., Li N., Li Y., Du Q., Lu J., Weng X., Yi W., Zhang H., Fan J., Zhou J., Gao Q., Onuchic J.N., Bode A.M., Luo X, & Cao Y. (2018). Targeting CPT1A-mediated fatty acid oxidation sensitizes nasopharyngeal carcinoma to radiation therapy. Theranostics, 8(9), 2329-2347.

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NMR and Mass Spectrometry Analysis

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NMR spectra were recorded in CD₃OD on a 600 MHz Bruker Avance III spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany) equipped with a CryoProbeTM at 600.15 MHz. The chemical shift values were reported in ppm (δ) and referenced to solvent residual protons (CD₃OD ¹H δ 3.34, ¹³C 49.0 ppm). High-resolution mass spectra were acquired on a QExactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific, Milan, Italy) on-line with the UHPLC apparatus Infinity 1290 (Agilent Technologies, Santa Clara, CA, USA). HPLC analyses were performed on a Shimadzu System LC 6A with a UV–VIS detector SPD 10A VP, a CR 3A recorder and a system controller SCL 10A VP (polyphenols) or on a LC-4000 (Jasco) instrument connected to a UV/Vis detector model UV 4070 (Jasco) and interfaced to a PC running the ChromNav software package (Chromatography Data System, Jasco) and Chemstation integration software Class–VP 5.0 (glutamic and aspartic acids, AMP and GMP). The chemical standards and solvents (HPLC/MS-grade) were purchased from Merck (Milan, Italy).

Morelli C.F., Cutignano A., Speranza G., Abbamondi G.R., Rabuffetti M., Iodice C., De Prisco R, & Tommonaro G. (2023). Taste Compounds and Polyphenolic Profile of Tomato Varieties Cultivated with Beneficial Microorganisms: A Chemical Investigation on Nutritional Properties and Sensory Qualities. Biomolecules, 13(1), 117.

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